##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545393_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	434923
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209933712404265	31.0	31.0	33.0	30.0	34.0
2	31.586430241674964	31.0	31.0	34.0	30.0	34.0
3	31.551313680812466	31.0	31.0	34.0	30.0	34.0
4	35.37441340191252	37.0	35.0	37.0	33.0	37.0
5	35.27889764395077	37.0	35.0	37.0	33.0	37.0
6	35.34822945670843	37.0	35.0	37.0	32.0	37.0
7	35.79036978959494	37.0	35.0	37.0	35.0	37.0
8	35.805287372707355	37.0	35.0	37.0	35.0	37.0
9	37.449753174699886	39.0	37.0	39.0	35.0	39.0
10	36.88795258011188	39.0	37.0	39.0	32.0	39.0
11	36.588281144018595	39.0	35.0	39.0	32.0	39.0
12	36.238478995132475	38.0	35.0	39.0	32.0	39.0
13	36.09007571455177	38.0	35.0	39.0	31.0	39.0
14	37.197630385148635	39.0	35.0	41.0	31.0	41.0
15	37.28429170220936	39.0	35.0	41.0	32.0	41.0
16	37.32274908432067	39.0	35.0	41.0	32.0	41.0
17	37.23192841031631	39.0	35.0	41.0	32.0	41.0
18	36.98049079952084	39.0	35.0	40.0	31.0	41.0
19	36.76571944918986	38.0	35.0	40.0	31.0	41.0
20	36.495956755563626	38.0	35.0	40.0	31.0	41.0
21	36.31991180047962	38.0	35.0	40.0	30.0	41.0
22	36.26940400944535	38.0	35.0	40.0	30.0	41.0
23	36.17654159471906	38.0	35.0	40.0	30.0	41.0
24	36.060654414689495	37.0	35.0	40.0	30.0	41.0
25	35.91611158756837	37.0	35.0	40.0	30.0	41.0
26	35.79375429673758	37.0	34.0	40.0	30.0	41.0
27	35.71868813560101	37.0	34.0	40.0	30.0	41.0
28	35.62132147529563	37.0	34.0	40.0	29.0	41.0
29	35.50915219475631	37.0	34.0	40.0	29.0	41.0
30	35.301221135695286	37.0	34.0	40.0	27.0	41.0
31	35.02543208797879	37.0	34.0	40.0	26.0	41.0
32	34.63410994589847	37.0	33.0	40.0	24.0	41.0
33	34.15234420805522	37.0	33.0	40.0	22.0	41.0
34	33.67949729032495	36.0	33.0	40.0	18.0	41.0
35	33.34760175939189	36.0	32.0	40.0	18.0	41.0
36	33.14706281341755	36.0	32.0	40.0	16.0	41.0
37	33.053345074875324	36.0	32.0	40.0	15.0	41.0
38	32.95292040200219	36.0	32.0	40.0	15.0	41.0
39	32.80904435957629	36.0	31.0	40.0	15.0	41.0
40	32.66988869294105	35.0	31.0	40.0	15.0	41.0
41	32.52157048489043	35.0	31.0	40.0	13.0	41.0
42	32.38054092333586	35.0	31.0	40.0	12.0	41.0
43	32.260057527424394	35.0	31.0	40.0	12.0	41.0
44	32.15023808812135	35.0	30.0	40.0	10.0	41.0
45	32.10116043529544	35.0	30.0	40.0	10.0	41.0
46	32.046624344999	35.0	30.0	39.0	10.0	41.0
47	31.98376264304256	35.0	30.0	39.0	10.0	41.0
48	31.900644481896794	35.0	30.0	39.0	10.0	41.0
49	31.820825755363593	35.0	30.0	39.0	10.0	41.0
50	31.690184239509062	35.0	30.0	39.0	10.0	41.0
51	31.536899635107822	35.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	5.0
15	33.0
16	62.0
17	191.0
18	440.0
19	875.0
20	1519.0
21	2272.0
22	3343.0
23	4572.0
24	6441.0
25	9497.0
26	12520.0
27	14082.0
28	13822.0
29	13394.0
30	13865.0
31	15415.0
32	18041.0
33	21874.0
34	31968.0
35	42789.0
36	35183.0
37	40852.0
38	54424.0
39	77415.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.43711185658151	21.387004136364368	24.623439091517348	23.552444915536775
2	40.301616607997275	22.26922926587005	25.44151493482754	11.987639191305128
3	26.286951943217534	23.00614131696875	37.679773201233324	13.027133538580394
4	23.56026239127386	25.21158917785447	37.55055492581445	13.677593505057217
5	21.561747711663905	28.453542351174804	36.11144961291999	13.873260324241302
6	19.865815328230514	36.78191312025347	33.753331049404146	9.598940502111867
7	76.8149764441062	4.315936384141561	16.835623777082382	2.033463394669861
8	77.3316196200247	3.6726041161309015	16.868503160329528	2.127273103514875
9	72.7016046518579	5.439353632711998	18.422341425953558	3.436700289476528
10	38.28884653145499	24.240382780400207	24.46938883434539	13.001381853799407
11	29.994044922894396	23.050516988064555	31.259556289274194	15.695881799766855
12	26.985006541387786	20.55444297036487	36.51312991035195	15.947420577895397
13	21.45736141799813	25.098465705423717	36.413801983339575	17.030370893238572
14	17.82913297296303	26.983397061088976	37.89107497189158	17.29639499405642
15	17.54563451461523	22.326480779356345	43.63186127199527	16.496023434033148
16	20.146324751737662	20.260367927196306	41.210513125311834	18.38279419575419
17	20.204725894008824	20.587782205125965	36.472433051367716	22.7350588494975
18	21.553010532898927	21.8486950563663	37.91199821577613	18.686296194958647
19	25.12697649928838	23.137428924200375	33.3677455549603	18.367849021550942
20	26.75646033895655	22.150587575271942	33.36015800498019	17.73279408079131
21	23.6802836364138	23.728568045378147	34.18421191797169	18.406936400236365
22	22.573421042345426	22.28923280672671	34.01360700629767	21.1237391446302
23	21.01061567219945	24.673332980780504	33.959114601895045	20.356936745124997
24	21.053841714510384	22.263021270431775	38.25481751942298	18.42831949563486
25	20.82506558632218	23.79593629217126	35.79461191981109	19.584386201695473
26	19.801206190521082	25.94298301078582	34.20329575580045	20.05251504289265
27	18.953239998804385	25.453011222676196	35.09402813831414	20.499720640205275
28	19.221793282948934	24.686438748927973	36.97390112732599	19.117866840797106
29	20.379239543551385	22.943141659558126	36.78858096720569	19.889037829684796
30	20.721828921441265	24.92119294679748	35.52996737353509	18.827010758226166
31	23.38459911294643	25.035925899527044	32.76855903228847	18.810915955238052
32	25.064666619148678	24.603665476417667	31.858282960432078	18.47338494400158
33	23.931592488785373	25.915161994192076	30.96961990973115	19.183625607291404
34	21.273190886662697	26.419159253477055	31.615711286825483	20.69193857303477
35	21.734651880907656	27.014437038280338	31.604674850490778	19.64623623032123
36	23.742823442310478	28.113712082368604	28.813146235080694	19.330318240240228
37	22.355681350491928	29.32840985645735	29.646167252594136	18.669741540456585
38	22.16162401160665	28.857981757690442	28.474235669302384	20.50615856140052
39	21.828001738238722	29.12124674942461	28.33191162573605	20.71883988660062
40	22.97992978067382	26.51319888807904	29.49901476813137	21.00785656311577
41	20.33624342699742	26.774624473757424	29.97473115930866	22.914400939936495
42	21.701772497660503	27.493142464298277	28.091409283942216	22.713675754099004
43	21.512543599671666	26.9328133945549	29.568912198251184	21.985730807522252
44	21.751896312680636	27.422555256907543	29.722272678152223	21.103275752259595
45	20.274393398371664	29.44291288342994	28.52964777673289	21.7530459414655
46	21.08120287959018	28.95156154077848	29.042152289025875	20.925083290605464
47	21.235712988276088	28.252817165337312	29.48314989090023	21.028319955486374
48	21.795352280748546	26.23935731152411	30.652322365108308	21.312968042619037
49	20.984404135904516	26.622873474155195	30.885467082679003	21.507255307261286
50	20.111835888191703	28.81061705175399	30.257769766142513	20.819777293911795
51	20.11091618516381	28.767161083686076	29.662721907096202	21.459200824053912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	382.0
1	664.5
2	947.0
3	7626.5
4	14306.0
5	10121.5
6	5937.0
7	5982.0
8	6027.0
9	6347.5
10	6668.0
11	6636.0
12	6604.0
13	6548.5
14	6493.0
15	6051.5
16	5610.0
17	5211.5
18	4813.0
19	4489.0
20	4165.0
21	4051.0
22	3937.0
23	3845.5
24	3754.0
25	3788.5
26	3923.0
27	4023.0
28	4676.0
29	5329.0
30	5943.5
31	6558.0
32	7306.0
33	8054.0
34	9521.5
35	10989.0
36	11897.0
37	12805.0
38	13639.0
39	14473.0
40	17042.5
41	19612.0
42	21118.0
43	22624.0
44	23547.0
45	24470.0
46	25295.0
47	26120.0
48	25877.0
49	25634.0
50	24967.0
51	24300.0
52	23565.5
53	22831.0
54	22103.5
55	21376.0
56	20936.5
57	20497.0
58	20392.5
59	20288.0
60	19509.0
61	18730.0
62	17022.5
63	15315.0
64	13363.5
65	11412.0
66	10081.0
67	8750.0
68	7287.5
69	5825.0
70	5069.0
71	4313.0
72	3638.5
73	2964.0
74	2413.0
75	1477.5
76	1093.0
77	834.0
78	575.0
79	465.0
80	355.0
81	257.0
82	159.0
83	106.5
84	54.0
85	44.0
86	34.0
87	23.0
88	12.0
89	13.0
90	14.0
91	9.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.28276991540403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.32459078652953	29.572775425774918
2	9.604450077953873	8.314144057761236
3	5.548301828257625	7.204376143610711
4	3.9309589315341777	6.805711639219853
5	2.9492820147335075	6.382654742967482
6	2.3325350074790063	6.057514562900343
7	1.7653333939374307	5.348596337964119
8	1.3427995190511435	4.649606610048467
9	0.9884518710046978	3.8504641414632257
>10	3.1897534851568135	18.586905655124042
>50	0.013911822577653958	0.41478334937571965
>100	0.009096191685386515	0.7885747094494768
>500	0.0	0.0
>1k	0.0	0.0
>5k	5.350700991403832E-4	2.0238926243403754
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8739	2.0093211901876886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017934209043899724	0.0
2	0.0	0.0	0.0	0.050583666534076144	0.0
3	0.0	0.0	0.0	0.10507607093669454	0.0
4	0.0	0.0	0.0	0.16899543137520895	0.0
5	0.0	0.0	0.0	0.29062615681396475	0.0
6	0.0	0.0	0.0	0.5840114227116064	0.0
7	0.0	0.0	0.0	0.7661126222342806	0.0
8	0.0	0.0	0.0	1.0413337533310494	0.0
9	0.0	0.0	0.0	1.243208567953408	0.0
10	0.0	0.0	0.0	1.5441813838311609	0.0
11	0.0	0.0	0.0	1.6913338682939278	0.0
12	0.0	0.0	0.0	1.8210119952267412	0.0
13	0.0	0.0	0.0	1.8883802420198519	0.0
14	0.0	0.0	0.0	1.9286172494901397	0.0
15	0.0	0.0	0.0	1.9635659645500467	0.0
16	0.0	0.0	0.0	2.0040328977773076	0.0
17	0.0	0.0	0.0	2.054156712797438	0.0
18	0.0	0.0	0.0	2.1252037717021173	0.0
19	0.0	0.0	0.0	2.1670502594712167	0.0
20	0.0	0.0	0.0	2.214874816921616	0.0
21	2.299257569730734E-4	0.0	0.0	2.2666081122405575	0.0
22	2.299257569730734E-4	0.0	0.0	2.324089551483826	0.0
23	2.299257569730734E-4	0.0	0.0	2.384330099810771	0.0
24	2.299257569730734E-4	0.0	0.0	2.4335342118030088	0.0
25	2.299257569730734E-4	0.0	0.0	2.4827383237952465	0.0
26	2.299257569730734E-4	0.0	0.0	2.531252658516565	0.0
27	2.299257569730734E-4	0.0	0.0	2.5921829841144293	0.0
28	2.299257569730734E-4	0.0	0.0	2.650354200628617	0.0
29	2.299257569730734E-4	0.0	0.0	2.703926902003343	0.0
30	2.299257569730734E-4	0.0	0.0	2.7811819563462956	0.0
31	2.299257569730734E-4	0.0	0.0	2.8446414652708643	0.0
32	2.299257569730734E-4	0.0	0.0	2.909250602980298	0.0
33	2.299257569730734E-4	0.0	0.0	2.9738597406897314	0.0
34	2.299257569730734E-4	0.0	0.0	3.041687838996788	0.0
35	2.299257569730734E-4	0.0	0.0	3.127450146347744	0.0
36	2.299257569730734E-4	0.0	0.0	3.207694235531347	0.0
37	2.299257569730734E-4	0.0	0.0	3.2867886959300843	0.0
38	2.299257569730734E-4	0.0	0.0	3.3727809290380137	0.0
39	2.299257569730734E-4	0.0	0.0	3.487283956010604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	35	1.2095916E-7	45.0	1
ACGGGTA	30	2.1623782E-6	44.999996	5
CGTTTTT	5810	0.0	43.141136	1
TTACGGG	110	0.0	40.909092	3
GTTACGG	45	1.9235813E-8	40.0	2
CGACAGG	40	3.4516052E-7	39.375004	2
CGAGGGA	190	0.0	39.078945	4
GTTGATC	265	0.0	39.056602	16
CAGTAGC	260	0.0	38.942307	27
GTAGGGC	220	0.0	38.863636	4
TCGTTGA	35	6.2393356E-6	38.571426	24
AATGGTC	70	0.0	38.571426	10
TTCGTAC	35	6.2393356E-6	38.571426	33
CCAGTAG	265	0.0	38.207546	26
AGCATAT	260	0.0	38.07692	31
CATACGA	130	0.0	38.07692	18
ATACGAA	130	0.0	38.07692	19
ACATACG	130	0.0	38.07692	17
GGTACCT	280	0.0	37.767857	8
AGTAGCA	275	0.0	37.636364	28
>>END_MODULE