FastQCFastQC Report
Thu 26 May 2016
SRR1545392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545392_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences101000
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.5841584158415841No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2020.2No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1890.18712871287128713No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1060.10495049504950496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC207.0027326E-445.0000048
ACGCCGG207.0027326E-445.00000427
CCCTCGT207.0027326E-445.00000414
CCTAGGG207.0027326E-445.0000043
TATGGGT207.0027326E-445.0000044
TGGGTCA207.0027326E-445.0000046
GGCAAGT207.0027326E-445.0000042
ATAGCGG207.0027326E-445.0000042
TACGGGA406.708433E-945.0000044
GCCGATT207.0027326E-445.0000049
ACGGCGA302.1458236E-645.00000416
CTTGCGG207.0027326E-445.0000042
GGTATGC302.1458236E-645.0000048
GCGATGT207.0027326E-445.0000049
GCAGGGC302.1458236E-645.0000044
TAGATGG207.0027326E-445.0000042
TCTCACT406.708433E-945.00000444
TAGTGGT207.0027326E-445.00000415
CGGTAGT207.0027326E-445.00000412
CGGTAGG207.0027326E-445.00000431