##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545392_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	101000
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27412871287129	31.0	31.0	33.0	30.0	34.0
2	31.62957425742574	31.0	31.0	34.0	30.0	34.0
3	31.743039603960398	31.0	31.0	34.0	30.0	34.0
4	35.47017821782178	37.0	35.0	37.0	33.0	37.0
5	35.33132673267327	37.0	35.0	37.0	33.0	37.0
6	35.400415841584156	37.0	35.0	37.0	33.0	37.0
7	35.80243564356436	37.0	35.0	37.0	35.0	37.0
8	35.805554455445545	37.0	35.0	37.0	35.0	37.0
9	37.464564356435645	39.0	37.0	39.0	35.0	39.0
10	36.88932673267327	39.0	37.0	39.0	32.0	39.0
11	36.55039603960396	39.0	35.0	39.0	32.0	39.0
12	35.965455445544556	37.0	35.0	39.0	31.0	39.0
13	35.7329801980198	37.0	35.0	39.0	30.0	39.0
14	36.7789801980198	39.0	35.0	41.0	31.0	41.0
15	36.92878217821782	38.0	35.0	41.0	31.0	41.0
16	37.04422772277228	38.0	35.0	41.0	32.0	41.0
17	36.99106930693069	38.0	35.0	41.0	32.0	41.0
18	36.90814851485148	38.0	35.0	40.0	32.0	41.0
19	36.84371287128713	38.0	35.0	40.0	31.0	41.0
20	36.676742574257425	38.0	35.0	40.0	31.0	41.0
21	36.50437623762376	38.0	35.0	40.0	31.0	41.0
22	36.443633663366334	38.0	35.0	40.0	31.0	41.0
23	36.33760396039604	38.0	35.0	40.0	30.0	41.0
24	36.243752475247526	38.0	35.0	40.0	30.0	41.0
25	36.124465346534656	38.0	35.0	40.0	30.0	41.0
26	36.05040594059406	38.0	35.0	40.0	30.0	41.0
27	35.989930693069304	38.0	34.0	40.0	30.0	41.0
28	35.92824752475248	38.0	35.0	40.0	30.0	41.0
29	35.9239900990099	38.0	35.0	40.0	30.0	41.0
30	35.82618811881188	37.0	35.0	40.0	30.0	41.0
31	35.722900990099006	37.0	34.0	40.0	29.0	41.0
32	35.55970297029703	37.0	34.0	40.0	29.0	41.0
33	35.345257425742574	37.0	34.0	40.0	27.0	41.0
34	35.14177227722772	37.0	34.0	40.0	27.0	41.0
35	34.98677227722772	37.0	34.0	40.0	26.0	41.0
36	34.80158415841584	37.0	34.0	40.0	25.0	41.0
37	34.72972277227723	37.0	33.0	40.0	25.0	41.0
38	34.67546534653465	37.0	33.0	40.0	25.0	41.0
39	34.573613861386136	37.0	33.0	40.0	24.0	41.0
40	34.39863366336634	36.0	33.0	40.0	23.0	41.0
41	34.35720792079208	36.0	33.0	40.0	23.0	41.0
42	34.219683168316834	36.0	33.0	40.0	23.0	41.0
43	34.16830693069307	36.0	33.0	40.0	23.0	41.0
44	34.145455445544556	36.0	33.0	40.0	23.0	41.0
45	34.1130198019802	36.0	33.0	40.0	23.0	41.0
46	34.05269306930693	35.0	33.0	40.0	23.0	41.0
47	33.98457425742574	35.0	33.0	40.0	23.0	41.0
48	33.93152475247525	35.0	33.0	40.0	23.0	41.0
49	33.83461386138614	36.0	33.0	40.0	23.0	41.0
50	33.72119801980198	35.0	33.0	39.0	23.0	41.0
51	33.53921782178218	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	6.0
17	35.0
18	66.0
19	108.0
20	187.0
21	320.0
22	442.0
23	681.0
24	921.0
25	1274.0
26	1749.0
27	2042.0
28	2195.0
29	2516.0
30	2905.0
31	3466.0
32	4362.0
33	5407.0
34	8717.0
35	12567.0
36	7795.0
37	9526.0
38	13085.0
39	20612.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45247524752475	23.292079207920793	28.879207920792076	15.376237623762377
2	30.694059405940592	25.87821782178218	29.906930693069306	13.520792079207922
3	27.475247524752476	25.725742574257428	33.05841584158416	13.740594059405941
4	25.684158415841583	27.73663366336634	31.716831683168316	14.862376237623762
5	22.904950495049505	32.30990099009901	30.10990099009901	14.675247524752475
6	21.898019801980197	40.63267326732673	27.384158415841586	10.085148514851486
7	85.01287128712872	5.068316831683169	7.931683168316832	1.9871287128712871
8	85.43861386138613	4.395049504950495	7.903960396039604	2.262376237623762
9	80.22178217821782	6.364356435643565	9.744554455445545	3.669306930693069
10	46.2	24.38217821782178	16.246534653465346	13.171287128712873
11	37.66237623762376	23.281188118811883	23.15148514851485	15.904950495049505
12	35.05148514851485	21.704950495049506	27.545544554455446	15.698019801980198
13	21.93069306930693	33.004950495049506	27.10693069306931	17.957425742574255
14	17.57920792079208	35.050495049504946	28.639603960396038	18.73069306930693
15	17.01881188118812	23.75148514851485	41.52970297029703	17.7
16	18.04059405940594	19.893069306930695	41.44158415841584	20.624752475247522
17	18.706930693069307	20.91980198019802	29.93069306930693	30.442574257425743
18	21.642574257425743	23.877227722772275	32.71386138613862	21.766336633663368
19	28.0950495049505	24.478217821782177	26.211881188118813	21.214851485148515
20	30.41980198019802	22.036633663366338	27.11782178217822	20.425742574257423
21	24.222772277227723	26.892079207920794	26.736633663366337	22.14851485148515
22	23.401980198019803	25.243564356435645	25.587128712871287	25.76732673267327
23	22.304950495049507	28.39207920792079	25.594059405940595	23.708910891089108
24	20.805940594059404	23.447524752475246	35.61188118811881	20.134653465346535
25	20.262376237623762	25.56831683168317	32.298019801980196	21.871287128712872
26	19.440594059405942	31.153465346534652	28.16138613861386	21.244554455445545
27	19.67920792079208	31.194059405940592	28.36237623762376	20.764356435643563
28	18.23861386138614	28.276237623762373	33.90396039603961	19.58118811881188
29	19.550495049504953	24.142574257425743	33.056435643564356	23.250495049504952
30	19.645544554455448	30.16336633663366	29.493069306930693	20.698019801980198
31	25.402970297029704	27.225742574257428	26.430693069306933	20.94059405940594
32	28.110891089108915	26.4970297029703	25.994059405940597	19.398019801980197
33	26.914851485148517	27.104950495049508	24.886138613861387	21.094059405940595
34	21.046534653465347	26.901980198019803	27.763366336633666	24.288118811881187
35	22.285148514851485	25.425742574257427	29.546534653465343	22.742574257425744
36	26.244554455445545	27.30792079207921	25.532673267326732	20.914851485148514
37	21.750495049504952	31.61881188118812	27.63960396039604	18.99108910891089
38	21.956435643564358	30.53861386138614	25.79009900990099	21.714851485148515
39	22.395049504950496	30.557425742574257	26.550495049504953	20.497029702970295
40	24.871287128712872	26.864356435643565	26.662376237623764	21.601980198019803
41	19.442574257425743	25.34851485148515	28.299009900990097	26.90990099009901
42	21.56930693069307	26.966336633663367	25.233663366336636	26.23069306930693
43	21.76831683168317	26.484158415841584	27.685148514851488	24.062376237623763
44	20.924752475247523	28.63069306930693	28.1	22.344554455445547
45	19.57128712871287	32.757425742574256	24.73168316831683	22.93960396039604
46	20.82079207920792	31.99108910891089	26.320792079207923	20.867326732673266
47	22.3	27.401980198019803	27.212871287128714	23.085148514851486
48	22.184158415841583	24.7019801980198	29.895049504950492	23.21881188118812
49	21.006930693069307	26.83960396039604	29.724752475247524	22.42871287128713
50	19.93861386138614	31.426732673267328	27.70891089108911	20.925742574257423
51	20.417821782178216	30.76930693069307	25.833663366336634	22.97920792079208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	132.5
2	178.0
3	589.0
4	1000.0
5	727.5
6	455.0
7	462.0
8	469.0
9	496.0
10	523.0
11	551.0
12	579.0
13	561.0
14	543.0
15	514.0
16	485.0
17	464.0
18	443.0
19	434.5
20	426.0
21	502.5
22	579.0
23	559.5
24	540.0
25	631.5
26	840.5
27	958.0
28	1156.0
29	1354.0
30	1595.0
31	1836.0
32	2046.0
33	2256.0
34	2656.5
35	3057.0
36	3125.5
37	3194.0
38	3615.0
39	4036.0
40	5192.0
41	6348.0
42	6934.0
43	7520.0
44	7915.5
45	8311.0
46	7803.5
47	7296.0
48	7194.5
49	7093.0
50	6938.0
51	6783.0
52	6433.0
53	6083.0
54	5507.0
55	4931.0
56	4740.5
57	4550.0
58	4359.0
59	4168.0
60	4019.0
61	3870.0
62	3478.5
63	3087.0
64	2624.5
65	2162.0
66	1854.0
67	1546.0
68	1366.5
69	1187.0
70	1053.5
71	920.0
72	740.5
73	561.0
74	452.0
75	283.0
76	223.0
77	189.0
78	155.0
79	115.5
80	76.0
81	57.5
82	39.0
83	28.5
84	18.0
85	11.0
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	101000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.556435643564356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.69208032687125	29.015841584158412
2	11.881683039207164	10.825742574257426
3	7.35677649308876	10.054455445544555
4	5.0269494914370165	9.16039603960396
5	3.6273146135790664	8.262376237623762
6	2.6014952621055376	7.11089108910891
7	1.897331131009302	6.05049504950495
8	1.1823002694949145	4.308910891089108
9	0.9258454316265322	3.796039603960396
>10	1.7886638268277841	10.037623762376239
>50	0.010866730418151786	0.300990099009901
>100	0.006520038250891072	0.49207920792079207
>500	0.0021733460836303575	0.5841584158415841
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.5841584158415841	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	202	0.2	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	189	0.18712871287128713	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	106	0.10495049504950496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02277227722772277	0.0
2	0.0	0.0	0.0	0.07920792079207921	0.0
3	0.0	0.0	0.0	0.11485148514851486	0.0
4	0.0	0.0	0.0	0.1900990099009901	0.0
5	0.0	0.0	0.0	0.3207920792079208	0.0
6	0.0	0.0	0.0	0.5633663366336633	0.0
7	0.0	0.0	0.0	0.691089108910891	0.0
8	0.0	0.0	0.0	0.9742574257425742	0.0
9	0.0	0.0	0.0	1.1445544554455445	0.0
10	0.0	0.0	0.0	1.388118811881188	0.0
11	0.0	0.0	0.0	1.5653465346534654	0.0
12	0.0	0.0	0.0	1.7237623762376237	0.0
13	0.0	0.0	0.0	1.7900990099009901	0.0
14	0.0	0.0	0.0	1.8227722772277228	0.0
15	0.0	0.0	0.0	1.8584158415841585	0.0
16	0.0	0.0	0.0	1.9247524752475247	0.0
17	0.0	0.0	0.0	1.998019801980198	0.0
18	0.0	0.0	0.0	2.091089108910891	0.0
19	0.0	0.0	0.0	2.1405940594059407	0.0
20	0.0	0.0	0.0	2.199009900990099	0.0
21	0.0	0.0	0.0	2.271287128712871	0.0
22	0.0	0.0	0.0	2.3386138613861385	0.0
23	0.0	0.0	0.0	2.4326732673267326	0.0
24	0.0	0.0	0.0	2.493069306930693	0.0
25	0.0	0.0	0.0	2.5722772277227723	0.0
26	0.0	0.0	0.0	2.616831683168317	0.0
27	0.0	0.0	0.0	2.6861386138613863	0.0
28	0.0	0.0	0.0	2.758415841584158	0.0
29	0.0	0.0	0.0	2.8356435643564355	0.0
30	0.0	0.0	0.0	2.906930693069307	0.0
31	0.0	0.0	0.0	2.9673267326732673	0.0
32	0.0	0.0	0.0	3.0584158415841585	0.0
33	0.0	0.0	0.0	3.122772277227723	0.0
34	0.0	0.0	0.0	3.207920792079208	0.0
35	0.0	0.0	0.0	3.299009900990099	0.0
36	0.0	0.0	0.0	3.398019801980198	0.0
37	0.0	0.0	0.0	3.4742574257425742	0.0
38	0.0	0.0	0.0	3.5495049504950495	0.0
39	0.0	0.0	0.0	3.627722772277228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	20	7.0027326E-4	45.000004	8
ACGCCGG	20	7.0027326E-4	45.000004	27
CCCTCGT	20	7.0027326E-4	45.000004	14
CCTAGGG	20	7.0027326E-4	45.000004	3
TATGGGT	20	7.0027326E-4	45.000004	4
TGGGTCA	20	7.0027326E-4	45.000004	6
GGCAAGT	20	7.0027326E-4	45.000004	2
ATAGCGG	20	7.0027326E-4	45.000004	2
TACGGGA	40	6.708433E-9	45.000004	4
GCCGATT	20	7.0027326E-4	45.000004	9
ACGGCGA	30	2.1458236E-6	45.000004	16
CTTGCGG	20	7.0027326E-4	45.000004	2
GGTATGC	30	2.1458236E-6	45.000004	8
GCGATGT	20	7.0027326E-4	45.000004	9
GCAGGGC	30	2.1458236E-6	45.000004	4
TAGATGG	20	7.0027326E-4	45.000004	2
TCTCACT	40	6.708433E-9	45.000004	44
TAGTGGT	20	7.0027326E-4	45.000004	15
CGGTAGT	20	7.0027326E-4	45.000004	12
CGGTAGG	20	7.0027326E-4	45.000004	31
>>END_MODULE