FastQCFastQC Report
Thu 26 May 2016
SRR1545391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545391_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2851143
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111510.39110630368241794No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC73470.257686127984461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTG253.8922633E-545.00000433
TACCCGC207.0350466E-445.018
ACAATCG406.8212103E-945.01
GTCGTAC453.8562575E-1045.09
GTACGTC406.8212103E-945.09
GTCGATC302.1666383E-644.99999610
CGTTAGT302.1666383E-644.9999962
CACTACG302.1666383E-644.99999636
TGTTACG2150.043.9534871
ATATACG1050.040.7142831
ACGGGAT5050.040.544555
ATCGTTC451.9299478E-840.028
ATCGTAC451.9299478E-840.040
GTTACGG4350.039.8275872
TCGTAAG1300.039.8076931
CGTTTTT98150.039.6586841
TAATCCG403.4606273E-739.3751
CTAGCGA356.251543E-638.5714314
GGCACCG11850.038.5443048
GCGATAA2750.038.4545449
Produced by FastQC (version 0.11.5)