##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545391_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2851143
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320488660161907	31.0	31.0	33.0	30.0	34.0
2	31.67108489472468	31.0	31.0	34.0	30.0	34.0
3	31.77050642496711	31.0	31.0	34.0	30.0	34.0
4	35.476058899886816	37.0	35.0	37.0	33.0	37.0
5	35.340550438894155	37.0	35.0	37.0	33.0	37.0
6	35.41030702423554	37.0	35.0	37.0	33.0	37.0
7	35.87087494383832	37.0	35.0	37.0	35.0	37.0
8	35.90786887925299	37.0	35.0	37.0	35.0	37.0
9	37.589410632858474	39.0	37.0	39.0	35.0	39.0
10	36.88547785923049	39.0	37.0	39.0	32.0	39.0
11	36.54700167616987	39.0	35.0	39.0	32.0	39.0
12	35.68996714650931	37.0	35.0	39.0	31.0	39.0
13	35.31648395047179	37.0	35.0	39.0	30.0	39.0
14	36.296023384305876	38.0	35.0	40.0	31.0	41.0
15	36.55198809740514	38.0	35.0	40.0	31.0	41.0
16	36.73949956210544	38.0	35.0	41.0	32.0	41.0
17	36.702310266444016	38.0	35.0	40.0	32.0	41.0
18	36.62620184255928	38.0	35.0	40.0	31.0	41.0
19	36.57089244559112	37.0	35.0	40.0	31.0	41.0
20	36.417560957131926	37.0	35.0	40.0	31.0	41.0
21	36.21751066151364	37.0	35.0	40.0	31.0	41.0
22	36.14047103214395	37.0	35.0	40.0	30.0	41.0
23	36.089912361463455	37.0	34.0	40.0	31.0	41.0
24	36.02684607541607	37.0	34.0	40.0	31.0	41.0
25	35.90985334653506	37.0	34.0	40.0	30.0	41.0
26	35.82136006506864	37.0	34.0	40.0	30.0	41.0
27	35.78489293592079	37.0	34.0	40.0	30.0	41.0
28	35.796900400997075	37.0	34.0	40.0	30.0	41.0
29	35.81104490374562	36.0	34.0	40.0	30.0	41.0
30	35.725948154827734	36.0	34.0	40.0	30.0	41.0
31	35.5475309375924	36.0	34.0	40.0	30.0	41.0
32	35.35392297054199	36.0	34.0	40.0	29.0	41.0
33	35.18916588890841	36.0	34.0	40.0	28.0	41.0
34	35.02249027846025	36.0	34.0	40.0	27.0	41.0
35	34.87332343554848	36.0	34.0	40.0	27.0	41.0
36	34.70723460731363	36.0	34.0	40.0	25.0	41.0
37	34.63615293936502	36.0	33.0	40.0	25.0	41.0
38	34.62343523281715	36.0	33.0	40.0	26.0	41.0
39	34.55309432041816	36.0	33.0	40.0	25.0	41.0
40	34.41066091739348	36.0	33.0	40.0	24.0	41.0
41	34.392085910808404	36.0	33.0	40.0	24.0	41.0
42	34.32279299915858	35.0	33.0	40.0	24.0	41.0
43	34.23903606378214	35.0	33.0	40.0	24.0	41.0
44	34.20741751641359	35.0	33.0	40.0	24.0	41.0
45	34.15396421715782	35.0	33.0	40.0	24.0	41.0
46	34.14289251714137	35.0	33.0	40.0	24.0	41.0
47	34.08364540116017	35.0	33.0	40.0	24.0	41.0
48	34.053337205464615	35.0	33.0	40.0	24.0	41.0
49	34.01110256483102	35.0	33.0	39.0	24.0	41.0
50	33.86290375473977	35.0	33.0	39.0	24.0	41.0
51	33.681869692260264	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	7.0
14	28.0
15	97.0
16	228.0
17	623.0
18	1432.0
19	2844.0
20	5283.0
21	8233.0
22	12409.0
23	17905.0
24	25207.0
25	35612.0
26	46872.0
27	54600.0
28	60559.0
29	68849.0
30	83042.0
31	100781.0
32	125787.0
33	163934.0
34	277147.0
35	431121.0
36	198097.0
37	239053.0
38	338384.0
39	552701.0
40	300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.686044158430498	24.6858891328846	29.647723737462485	14.980342971222418
2	29.367064366817097	26.636510339888247	30.593554935687195	13.402870357607458
3	28.303034958260596	25.89673685255352	32.775767472904725	13.024460716281155
4	25.490478730810768	28.58997251277821	31.632857418936894	14.286691337474128
5	22.68290296207521	32.721508531841444	30.768011285298563	13.827577220784788
6	20.570206404940052	41.40504352114222	27.47129835297633	10.553451720941391
7	86.76927113091135	4.659324348164929	6.9479503483339835	1.6234541725897298
8	88.49265014066289	3.3695959830846784	6.444362839745323	1.693391036507113
9	83.91055797622217	5.165752822639902	8.233224359493718	2.6904648416442107
10	52.000162741749534	18.506086857095557	17.350760729994953	12.142989671159953
11	46.33166417819099	22.177421476229007	17.683223885999404	13.807690459580597
12	38.61658289324667	21.523753806806603	25.153666441844553	14.705996858102171
13	20.803867080676067	39.517695184001646	25.34306416759875	14.335373567723542
14	16.136335497728453	41.361236528648334	27.66150277274763	14.84092520087558
15	15.374886492890747	23.997393326115176	46.737396195139986	13.890323985854094
16	16.08109449438348	19.20836660946154	46.08621875507472	18.62432014108026
17	16.872391177853935	19.611468102441723	29.864093102310196	33.652047617394146
18	21.73114431650745	22.50027445133408	35.47422910741411	20.294352124744357
19	29.611141917469592	23.201537067765454	26.814649423055947	20.37267159170901
20	31.22428443610159	22.60840652327856	25.838619809669318	20.328689230950534
21	22.024886159691043	28.460445512554088	28.21391280619737	21.300755521557495
22	22.163321867756196	24.363772704490795	26.492743436579648	26.98016199117336
23	19.284686878209897	30.725396797003867	25.586650687110396	24.403265637675837
24	20.06532117119345	24.22512655450814	38.31754492847254	17.392007345825867
25	17.163642791680388	24.795880108433703	37.594045616091506	20.446431483794395
26	16.669455022073603	34.08548782014792	29.670732053776327	19.57432510400215
27	17.575652992501603	34.249772810413226	30.042968732189163	18.13160546489601
28	16.61270585165318	28.399697945701075	37.868181287294256	17.11941491535149
29	16.84282408844453	24.957534574730207	36.37471007241657	21.824931264408693
30	18.206277271957248	31.136214493625893	30.855625270286335	19.801882964130527
31	28.40390678405117	26.531184160177162	26.296751864076967	18.768157191694698
32	28.61077118895825	25.451757417989906	28.603335574539756	17.334135818512085
33	28.503691326601295	27.198495480584455	25.183128310295206	19.114684882519047
34	20.267485706609595	27.29859568601084	28.020025652869744	24.413892954509823
35	21.026058671908075	25.67826306853076	31.47432450775005	21.821353751811117
36	30.278207722306455	24.69346504191477	26.77687509886386	18.251452136914914
37	20.914173719101427	32.41605208858342	29.336094331290997	17.333679861024155
38	21.645915339918062	33.37415906532924	24.286295005196163	20.693630589556538
39	21.529611106843817	31.54727770581833	27.477436242236884	19.445674945100965
40	24.492878820879906	26.17704548666973	25.62793237659423	23.702143315856134
41	18.029786650476666	24.459699145220004	27.879906409464557	29.63060779483877
42	21.393770849094558	26.07031636084195	25.75584598878415	26.780066801279347
43	21.766989589789077	26.17820291721601	26.671338477235267	25.383469015759648
44	19.19612590459335	30.702423554342946	28.84979813359063	21.251652407473074
45	18.245314247654363	37.38911026209489	23.63932640348099	20.726249086769762
46	22.07939061632475	32.97915958617298	26.047728928363117	18.89372086913915
47	22.13428088314055	27.56469949069549	26.783819682141512	23.517199944022448
48	23.157870369883234	24.87335780772834	31.27640388433691	20.69236793805151
49	22.53689134497989	24.040077961715706	31.986049103815557	21.436981589488845
50	20.65838156837451	32.49286338847262	26.441711271584765	20.407043771568105
51	19.643350052943678	33.82103247715039	24.539140969078016	21.996476500827914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1522.0
1	2834.5
2	4147.0
3	11756.0
4	19365.0
5	14596.0
6	9827.0
7	10634.5
8	11442.0
9	12204.5
10	12967.0
11	13424.0
12	13881.0
13	13924.5
14	13968.0
15	13958.0
16	13948.0
17	13071.5
18	12195.0
19	12633.5
20	13072.0
21	12935.5
22	12799.0
23	12882.5
24	12966.0
25	14368.0
26	20911.0
27	26052.0
28	29780.5
29	33509.0
30	42157.0
31	50805.0
32	56974.0
33	63143.0
34	67878.5
35	72614.0
36	78143.0
37	83672.0
38	102671.5
39	121671.0
40	161726.0
41	201781.0
42	246066.5
43	290352.0
44	292122.0
45	293892.0
46	274772.0
47	255652.0
48	244441.0
49	233230.0
50	225165.0
51	217100.0
52	197190.5
53	177281.0
54	160134.0
55	142987.0
56	124798.0
57	106609.0
58	96230.5
59	85852.0
60	76347.5
61	66843.0
62	59928.5
63	53014.0
64	42993.5
65	32973.0
66	29073.0
67	25173.0
68	22258.0
69	19343.0
70	14836.5
71	10330.0
72	9248.0
73	8166.0
74	6508.0
75	4342.0
76	3834.0
77	2621.5
78	1409.0
79	1032.0
80	655.0
81	478.0
82	301.0
83	215.5
84	130.0
85	86.5
86	43.0
87	23.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2851143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.623647066184231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54667956312184	12.584328938472503
2	7.100437104629433	2.2186944667673862
3	2.5172825469878273	1.1798740224000943
4	1.3371826662014592	0.8356668015900335
5	0.8301831635637724	0.648524437390434
6	0.5867970760678137	0.550074624957143
7	0.43194783714350343	0.47240203909722056
8	0.29876609318342506	0.3734252796192436
9	0.24690236598171675	0.3471763883313775
>10	2.957037061812107	11.226794756722
>50	1.3001197856655056	14.906172490592542
>100	1.8255975350796618	51.82440080332024
>500	0.019481497293840667	1.948087219746994
>1k	0.0011326451915023644	0.2296947124277018
>5k	2.2652903830047287E-4	0.2600257399393413
>10k+	2.2652903830047287E-4	0.3946572786257786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11151	0.39110630368241794	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7347	0.257686127984461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031601361278617034	0.0
2	0.0	0.0	0.0	0.09992483716179791	0.0
3	0.0	0.0	0.0	0.1420132206627307	0.0
4	0.0	0.0	0.0	0.22362961100162285	0.0
5	0.0	0.0	0.0	0.3770768425154403	0.0
6	0.0	0.0	0.0	0.5674215568984088	0.0
7	0.0	0.0	0.0	0.6777632689766876	0.0
8	0.0	0.0	0.0	0.9192453693132895	0.0
9	0.0	0.0	0.0	1.0315161323020277	0.0
10	0.0	0.0	0.0	1.2149513370602596	0.0
11	0.0	0.0	0.0	1.389092023795369	0.0
12	0.0	0.0	0.0	1.5426444762679388	0.0
13	7.014730583488798E-5	0.0	0.0	1.6065135982306045	0.0
14	7.014730583488798E-5	0.0	0.0	1.631205449884485	0.0
15	7.014730583488798E-5	0.0	0.0	1.6663141764548464	0.0
16	7.014730583488798E-5	0.0	0.0	1.738565901464781	0.0
17	7.014730583488798E-5	0.0	0.0	1.8242157618891792	0.0
18	7.014730583488798E-5	0.0	0.0	1.9440273602551679	0.0
19	7.014730583488798E-5	0.0	0.0	2.0020041085277027	0.0
20	7.014730583488798E-5	0.0	0.0	2.061980055016532	0.0
21	7.014730583488798E-5	0.0	0.0	2.151347722650179	0.0
22	7.014730583488798E-5	0.0	0.0	2.238295308232523	0.0
23	7.014730583488798E-5	0.0	0.0	2.3468833376649294	0.0
24	7.014730583488798E-5	0.0	0.0	2.4287101699213265	0.0
25	7.014730583488798E-5	0.0	0.0	2.502926019494638	0.0
26	7.014730583488798E-5	0.0	0.0	2.5732136199411957	0.0
27	1.0522095875233196E-4	0.0	0.0	2.642028126965221	0.0
28	1.0522095875233196E-4	0.0	0.0	2.718383469366496	0.0
29	1.4029461166977596E-4	0.0	0.0	2.798491692629938	0.0
30	1.4029461166977596E-4	0.0	0.0	2.9055014076810597	0.0
31	1.4029461166977596E-4	0.0	0.0	3.0065135280832984	0.0
32	1.4029461166977596E-4	0.0	0.0	3.0983012777682495	0.0
33	1.4029461166977596E-4	0.0	0.0	3.1885808603777503	0.0
34	1.4029461166977596E-4	0.0	0.0	3.284331932842372	0.0
35	1.4029461166977596E-4	0.0	0.0	3.395901222772762	0.0
36	2.1044191750466392E-4	0.0	0.0	3.493476125189091	0.0
37	2.1044191750466392E-4	0.0	0.0	3.597679948006817	0.0
38	2.1044191750466392E-4	0.0	0.0	3.698727142061973	0.0
39	2.1044191750466392E-4	0.0	0.0	3.797880358859587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTG	25	3.8922633E-5	45.000004	33
TACCCGC	20	7.0350466E-4	45.0	18
ACAATCG	40	6.8212103E-9	45.0	1
GTCGTAC	45	3.8562575E-10	45.0	9
GTACGTC	40	6.8212103E-9	45.0	9
GTCGATC	30	2.1666383E-6	44.999996	10
CGTTAGT	30	2.1666383E-6	44.999996	2
CACTACG	30	2.1666383E-6	44.999996	36
TGTTACG	215	0.0	43.953487	1
ATATACG	105	0.0	40.714283	1
ACGGGAT	505	0.0	40.54455	5
ATCGTTC	45	1.9299478E-8	40.0	28
ATCGTAC	45	1.9299478E-8	40.0	40
GTTACGG	435	0.0	39.827587	2
TCGTAAG	130	0.0	39.807693	1
CGTTTTT	9815	0.0	39.658684	1
TAATCCG	40	3.4606273E-7	39.375	1
CTAGCGA	35	6.251543E-6	38.57143	14
GGCACCG	1185	0.0	38.544304	8
GCGATAA	275	0.0	38.454544	9
>>END_MODULE