FastQCFastQC Report
Thu 26 May 2016
SRR1545390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545390_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3450595
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206690.5989981437983883No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC75970.2201649280776214No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG53330.1545530553426293No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39170.1135166543741007No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT35070.10163464561908889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA351.2128658E-745.00000417
CAGTACG1400.041.7857171
CTATGCG1550.040.645161
TATTACG1450.040.3448261
CGTTTTT168700.039.6784251
GTAGGGC16000.039.3754
GCGATCG1000.038.259
ACATACG10950.037.8082217
TATAGCG2100.037.5000041
CGTCGAA301.14039576E-437.49999614
CGTAAGG4150.037.4096372
CATACGA11100.037.297318
GGCGATA17150.037.2594768
TTACGAG1450.037.241381
CGCATCG552.752131E-936.8181821
CGAATAT4400.036.8181814
AACGTAG2700.036.6666641
GCTACGA4300.036.62790710
TTACGGG12350.036.437253
TAGGGCG15700.036.401275
Produced by FastQC (version 0.11.5)