FastQCFastQC Report
Thu 26 May 2016
SRR1545389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545389_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4949604
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT886181.7904058587313247No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT57320.11580724437753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT609200.042.8578451
TCTAGCG1550.037.7419361
GTTTTTT717300.037.578422
GGGCGAT115750.037.477327
GCCCTAC35450.037.3201726
AGGGCGA59950.037.1559646
CGTTAGG5100.036.617652
CTAATCC37300.036.01206611
TACGCTA250.002107780236.01
TACGAAT8350.035.83832612
GCGTTAG2450.035.8163261
GGCGATA23200.035.786648
CGTAAGG7500.035.72
ACTAATC37700.035.6896610
ATACTAT35350.035.57991445
TACACAA36600.035.53278730
TATGGGC18950.035.501324
CCCTACA37150.035.43068727
TAGGGCG24700.035.344135
ATCCTAG38100.035.2559114
Produced by FastQC (version 0.11.5)