##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545389_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4949604
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25654092731459	31.0	31.0	33.0	30.0	34.0
2	31.629888168831283	31.0	31.0	34.0	30.0	34.0
3	31.60056865155273	31.0	31.0	34.0	30.0	34.0
4	35.39741401534345	37.0	35.0	37.0	33.0	37.0
5	35.30323577401344	37.0	35.0	37.0	33.0	37.0
6	35.37178246987032	37.0	35.0	37.0	33.0	37.0
7	35.821633609476635	37.0	35.0	37.0	35.0	37.0
8	35.846222243234	37.0	35.0	37.0	35.0	37.0
9	37.50029295272915	39.0	37.0	39.0	35.0	39.0
10	36.93388723623142	39.0	37.0	39.0	32.0	39.0
11	36.60910771851647	39.0	35.0	39.0	32.0	39.0
12	36.1778160434653	38.0	35.0	39.0	32.0	39.0
13	35.98747960442896	38.0	35.0	39.0	31.0	39.0
14	37.0243914462652	39.0	35.0	41.0	31.0	41.0
15	37.14910485768154	39.0	35.0	41.0	31.0	41.0
16	37.22882012379172	39.0	35.0	41.0	32.0	41.0
17	37.14919516793667	39.0	35.0	41.0	32.0	41.0
18	36.926817579749816	39.0	35.0	40.0	31.0	41.0
19	36.730167100236706	38.0	35.0	40.0	31.0	41.0
20	36.465080438758335	38.0	35.0	40.0	31.0	41.0
21	36.29142654644695	38.0	35.0	40.0	31.0	41.0
22	36.23859221869063	38.0	35.0	40.0	30.0	41.0
23	36.14881998640699	37.0	35.0	40.0	30.0	41.0
24	36.037674125041114	37.0	35.0	40.0	30.0	41.0
25	35.89774939570923	37.0	35.0	40.0	30.0	41.0
26	35.795931553312144	37.0	34.0	40.0	30.0	41.0
27	35.740363875574694	37.0	34.0	40.0	30.0	41.0
28	35.644768955253795	37.0	34.0	40.0	29.0	41.0
29	35.557323979857784	37.0	34.0	40.0	29.0	41.0
30	35.37166104601499	37.0	34.0	40.0	28.0	41.0
31	35.08908328827923	37.0	34.0	40.0	27.0	41.0
32	34.70199999838371	37.0	33.0	40.0	24.0	41.0
33	34.25685792237116	36.0	33.0	40.0	23.0	41.0
34	33.78864693013825	36.0	33.0	40.0	20.0	41.0
35	33.45614032961021	36.0	33.0	40.0	18.0	41.0
36	33.24592775502848	36.0	32.0	40.0	18.0	41.0
37	33.14462470128923	36.0	32.0	40.0	17.0	41.0
38	33.05697082029189	36.0	32.0	40.0	16.0	41.0
39	32.9250594593022	36.0	32.0	40.0	15.0	41.0
40	32.78478722742264	35.0	31.0	40.0	15.0	41.0
41	32.65840438952288	35.0	31.0	40.0	15.0	41.0
42	32.53718438889253	35.0	31.0	40.0	15.0	41.0
43	32.40302113057933	35.0	31.0	40.0	13.0	41.0
44	32.28955932636227	35.0	31.0	40.0	12.0	41.0
45	32.252759412672205	35.0	31.0	40.0	12.0	41.0
46	32.20763337834703	35.0	31.0	39.0	12.0	41.0
47	32.14167234388852	35.0	31.0	39.0	12.0	41.0
48	32.05890552052245	35.0	31.0	39.0	12.0	41.0
49	31.978902958701344	35.0	30.0	39.0	12.0	41.0
50	31.862794276067337	35.0	30.0	39.0	11.0	41.0
51	31.7003069336456	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	14.0
13	21.0
14	84.0
15	269.0
16	813.0
17	2175.0
18	5021.0
19	9543.0
20	15902.0
21	24381.0
22	35702.0
23	51085.0
24	72612.0
25	105762.0
26	139818.0
27	153826.0
28	149691.0
29	147060.0
30	156591.0
31	177408.0
32	206793.0
33	255969.0
34	383454.0
35	508593.0
36	385141.0
37	459306.0
38	615265.0
39	886959.0
40	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.18611185864566	21.343768107509206	25.392475842511846	23.077644191333285
2	39.824115222147064	22.12047670884378	25.904375380333455	12.1510326886757
3	26.957005045252103	22.59423178096672	37.66317467013523	12.785588503645947
4	24.001738320883852	25.460400468401108	37.05350973532428	13.484351475390758
5	21.690078640634685	28.34295430503127	36.07522945269965	13.891737601634393
6	20.39183336687137	36.692349529376486	33.474556752418984	9.441260351333156
7	77.79238500696218	4.334185118647875	15.846095970505925	2.027333903884028
8	78.19964991138684	3.726277900211815	15.797910297470263	2.2761618909310726
9	73.44120863002374	5.391320194504449	17.744328637200066	3.4231425382717484
10	40.516776695671005	23.19567383572504	23.89471965838075	12.392829810223201
11	32.91297647246123	22.991314052598955	29.94760792984651	14.148101545093306
12	28.745289522151673	21.015297385406996	34.630346185270575	15.609066907170755
13	21.925895485780277	26.36823067057486	35.55294524572067	16.1529285979242
14	18.777360774720563	27.99512849916882	36.55359095394298	16.67391977216763
15	18.489903434699016	21.756467790150484	43.187798458220094	16.565830316930406
16	20.762085209240983	19.796007923058088	41.316133573514165	18.125773294186768
17	20.51459066220247	20.686240757846484	35.481242539807226	23.317926040143817
18	22.556006500722077	21.645125549437893	37.271486769446604	18.52738118039342
19	25.92193234044582	23.289358098142802	32.64384383073878	18.144865730672596
20	27.251533658046178	22.058673784811873	32.917643512491104	17.772149044650845
21	24.179287878383807	23.262083188877334	34.18180525149083	18.376823681248037
22	22.87841613187641	22.385669641450104	33.65942002632938	21.076494200344108
23	20.990749967068073	24.44516773463089	33.599051560488476	20.965030737812558
24	20.909935421096314	22.53669182423483	38.61856423261336	17.9348085220555
25	20.600395506387986	23.27931689080581	36.1698026751231	19.950484927683103
26	19.47115365188811	26.204621622255033	33.900227169688726	20.423997556168132
27	19.534896125023334	25.65225420053806	35.27195306937686	19.54089660506174
28	19.33302138918588	24.686762819813463	36.84064422123467	19.139571569765984
29	19.818595588657193	22.891811142871227	37.132849415832055	20.156743852639526
30	21.05679565476349	24.840047809885395	35.35127254624815	18.751883989102964
31	24.417872621728932	24.279336286296843	32.91855267613328	18.384238415840944
32	25.37223179874592	23.42460124082654	32.587031204920635	18.616135755506903
33	25.15876017556152	25.037437338421416	30.872853666677173	18.93094881933989
34	21.239638565024595	25.53497209069655	32.62780214336339	20.59758720091547
35	21.6133452292345	26.442438627413424	31.99761031387561	19.94660582947646
36	24.907043068495984	26.552245391752553	29.109177219026005	19.431534320725454
37	21.684522640599127	28.976944418179716	30.565334115618136	18.773198825603018
38	22.062795326656435	28.181830304000076	29.236419721658542	20.518954647684946
39	21.594939716389433	28.570386641032293	29.142654644694808	20.692018997883466
40	23.64639676224603	25.85366425273618	29.30996095849284	21.18997802652495
41	20.503276625766425	25.365423173247798	30.16184729121764	23.969452909768137
42	21.686704633340366	26.11291731621358	28.72732848930945	23.47304956113661
43	21.95438261323532	25.5408311452795	30.195951029617724	22.308835211867454
44	21.317260936430472	27.524929267068636	29.926293093346455	21.231516703154433
45	20.14639959075514	29.247228667182263	28.464984269448628	22.14138747261397
46	21.68666422606738	28.34620709050664	29.36628869703516	20.60083998639083
47	21.318190303709144	27.039132827595903	30.366631350710076	21.276045517984873
48	21.814331005066265	25.784810259568243	31.346871386074522	21.053987349290974
49	21.74113323005234	25.713410608202192	31.03318972588514	21.512266435860322
50	20.55645259701584	28.260503264503583	30.331699263213785	20.85134487526679
51	20.240952609542095	28.623259557734315	29.45284107577091	21.682946756952678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3900.0
1	6920.5
2	9941.0
3	76466.5
4	142992.0
5	100943.5
6	58895.0
7	59071.0
8	59247.0
9	60712.5
10	62178.0
11	62375.0
12	62572.0
13	61572.5
14	60573.0
15	58130.5
16	55688.0
17	52313.0
18	48938.0
19	47304.5
20	45671.0
21	43988.0
22	42305.0
23	40827.5
24	39350.0
25	41315.0
26	46176.5
27	49073.0
28	56169.5
29	63266.0
30	66262.5
31	69259.0
32	83426.5
33	97594.0
34	114727.0
35	131860.0
36	142005.5
37	152151.0
38	167417.5
39	182684.0
40	211523.5
41	240363.0
42	271459.5
43	302556.0
44	319326.0
45	336096.0
46	331394.5
47	326693.0
48	309970.5
49	293248.0
50	286680.5
51	280113.0
52	264350.0
53	248587.0
54	242586.5
55	236586.0
56	229400.5
57	222215.0
58	213872.0
59	205529.0
60	196638.0
61	187747.0
62	169997.5
63	152248.0
64	140923.0
65	129598.0
66	113697.5
67	97797.0
68	84624.0
69	71451.0
70	61248.5
71	51046.0
72	43096.5
73	35147.0
74	29864.0
75	18393.5
76	12206.0
77	9755.5
78	7305.0
79	5311.0
80	3317.0
81	2725.5
82	2134.0
83	1468.5
84	803.0
85	611.0
86	419.0
87	316.0
88	213.0
89	167.0
90	121.0
91	79.0
92	37.0
93	31.5
94	26.0
95	15.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4949604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.59385106325343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82801246838972	17.66981914044452
2	5.84357736799653	2.5237067872223107
3	2.057268203596701	1.3327302955690228
4	1.251152417232083	1.0806879582055644
5	0.8651145935885232	0.9340577843298793
6	0.6322656324003396	0.8191829939080006
7	0.5131372932314484	0.7756427199528628
8	0.40813549745426053	0.705057371652332
9	0.3633224719023921	0.7060978211573999
>10	3.830995088434509	20.050936695301957
>50	1.5656724607636494	24.358837154343586
>100	0.8367808830324808	26.107046713257702
>500	0.0027583966114963464	0.39016739613317697
>1k	0.0016169911168861073	0.6081320416721204
>5k	9.511712452271219E-5	0.11773212857554018
>10k+	9.511712452271219E-5	1.820164998274114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88618	1.7904058587313247	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5732	0.11580724437753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02163809468393835	0.0
2	0.0	0.0	0.0	0.0667730186091655	0.0
3	0.0	0.0	0.0	0.11536276437468533	0.0
4	0.0	0.0	0.0	0.1701954338165235	0.0
5	0.0	0.0	0.0	0.3033778055779816	0.0
6	0.0	0.0	0.0	0.6124934439199581	0.0
7	0.0	0.0	0.0	0.832086768961719	0.0
8	0.0	0.0	0.0	1.2536154407504116	0.0
9	0.0	0.0	0.0	1.5377189771141286	0.0
10	2.020363649293964E-5	0.0	0.0	1.833803269918159	0.0
11	2.020363649293964E-5	0.0	0.0	2.0489719985679664	0.0
12	4.040727298587928E-5	0.0	0.0	2.207449323218585	0.0
13	6.061090947881891E-5	0.0	0.0	2.29683021106335	0.0
14	6.061090947881891E-5	0.0	0.0	2.3501476077682173	0.0
15	6.061090947881891E-5	0.0	0.0	2.390857935301491	0.0
16	8.081454597175856E-5	0.0	0.0	2.448317077487411	0.0
17	8.081454597175856E-5	0.0	0.0	2.508544117872864	0.0
18	8.081454597175856E-5	0.0	0.0	2.592833689321408	0.0
19	1.2122181895763783E-4	0.0	0.0	2.6445549987433337	0.0
20	1.4142545545057746E-4	0.0	0.0	2.7001352027354106	0.0
21	1.4142545545057746E-4	0.0	0.0	2.766665777706661	0.0
22	1.4142545545057746E-4	0.0	0.0	2.840732309089778	0.0
23	1.4142545545057746E-4	0.0	0.0	2.9181930514037084	0.0
24	1.4142545545057746E-4	0.0	0.0	2.9871076554811253	0.0
25	1.4142545545057746E-4	0.0	0.0	3.0463649213149173	0.0
26	1.4142545545057746E-4	0.0	0.0	3.105500965329752	0.0
27	1.4142545545057746E-4	0.0	0.0	3.172375002121382	0.0
28	1.8183272843645673E-4	0.0	0.0	3.23779437708552	0.0
29	2.0203636492939638E-4	0.0	0.0	3.3163865230430556	0.0
30	2.0203636492939638E-4	0.0	0.0	3.414535789125756	0.0
31	2.0203636492939638E-4	0.0	0.0	3.4934107859941927	0.0
32	2.0203636492939638E-4	0.0	0.0	3.57531632833657	0.0
33	2.0203636492939638E-4	0.0	0.0	3.652595237922064	0.0
34	2.4244363791527565E-4	0.0	0.0	3.7399355584810423	0.0
35	2.626472744082153E-4	0.0	0.0	3.838468693657109	0.0
36	2.626472744082153E-4	0.0	0.0	3.923465392382906	0.0
37	2.626472744082153E-4	0.0	0.0	4.019048796631004	0.0
38	2.626472744082153E-4	0.0	0.0	4.139260433763994	0.0
39	2.828509109011549E-4	0.0	0.0	4.338427882311393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	60920	0.0	42.857845	1
TCTAGCG	155	0.0	37.741936	1
GTTTTTT	71730	0.0	37.57842	2
GGGCGAT	11575	0.0	37.47732	7
GCCCTAC	3545	0.0	37.32017	26
AGGGCGA	5995	0.0	37.155964	6
CGTTAGG	510	0.0	36.61765	2
CTAATCC	3730	0.0	36.012066	11
TACGCTA	25	0.0021077802	36.0	1
TACGAAT	835	0.0	35.838326	12
GCGTTAG	245	0.0	35.816326	1
GGCGATA	2320	0.0	35.78664	8
CGTAAGG	750	0.0	35.7	2
ACTAATC	3770	0.0	35.68966	10
ATACTAT	3535	0.0	35.579914	45
TACACAA	3660	0.0	35.532787	30
TATGGGC	1895	0.0	35.50132	4
CCCTACA	3715	0.0	35.430687	27
TAGGGCG	2470	0.0	35.34413	5
ATCCTAG	3810	0.0	35.25591	14
>>END_MODULE