FastQCFastQC Report
Thu 26 May 2016
SRR1545388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545388_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3214258
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144560.4497461000330403No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC100530.3127626967094739No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG35570.11066317638472083No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG35320.10988539190071239No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32670.10164087637022291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT502.1827873E-1145.020
CACTACG253.892376E-545.01
CGGTCTA1350.045.031
CAATCGT351.2128476E-745.018
TATGTCG302.1667256E-644.9999961
CATACGA12150.042.0370418
ACATACG12200.041.8647517
TTACCGG1650.040.9090922
TACGATA451.9301297E-840.00000433
TGTAACG850.039.7058831
AATACGG1650.039.5454562
ATCGTAC403.4608092E-739.37529
TACGAAC403.4608092E-739.37516
TATAGCG800.039.3751
TACCGTA403.4608092E-739.37545
CGTTTTT116500.039.2639471
GCGATAT2650.039.0566029
TACGGGA7850.038.9808924
ACTTACG1450.038.7931061
GACCGAT20050.038.7157149
Produced by FastQC (version 0.11.5)