##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545388_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3214258
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351285739974823	31.0	31.0	33.0	30.0	34.0
2	31.708256462300163	31.0	31.0	34.0	30.0	34.0
3	31.8103198312021	31.0	31.0	34.0	30.0	34.0
4	35.51979772625595	37.0	35.0	37.0	33.0	37.0
5	35.39025803155814	37.0	35.0	37.0	33.0	37.0
6	35.45215940973002	37.0	35.0	37.0	33.0	37.0
7	35.84347833932435	37.0	35.0	37.0	35.0	37.0
8	35.864942079945045	37.0	35.0	37.0	35.0	37.0
9	37.52622564834559	39.0	37.0	39.0	35.0	39.0
10	36.974911161456234	39.0	37.0	39.0	33.0	39.0
11	36.62276705852486	39.0	35.0	39.0	32.0	39.0
12	35.82879501272144	37.0	35.0	39.0	31.0	39.0
13	35.480173340161244	37.0	35.0	39.0	30.0	39.0
14	36.47768660760897	38.0	35.0	41.0	31.0	41.0
15	36.70108155599208	38.0	35.0	41.0	31.0	41.0
16	36.85402851917923	38.0	35.0	41.0	32.0	41.0
17	36.803976220950524	38.0	35.0	41.0	32.0	41.0
18	36.74798787153987	38.0	35.0	40.0	32.0	41.0
19	36.66991946508339	38.0	35.0	40.0	31.0	41.0
20	36.50611307493051	38.0	35.0	40.0	31.0	41.0
21	36.31885492701581	38.0	35.0	40.0	31.0	41.0
22	36.238128053192995	38.0	35.0	40.0	31.0	41.0
23	36.187700862842995	37.0	35.0	40.0	31.0	41.0
24	36.105578332542066	37.0	35.0	40.0	31.0	41.0
25	35.9829674531416	37.0	35.0	40.0	30.0	41.0
26	35.87254196769519	37.0	34.0	40.0	30.0	41.0
27	35.82250086956305	37.0	34.0	40.0	30.0	41.0
28	35.79815839301014	37.0	34.0	40.0	30.0	41.0
29	35.80061432529685	37.0	35.0	40.0	30.0	41.0
30	35.71418162449934	36.0	34.0	40.0	30.0	41.0
31	35.535010257421774	36.0	34.0	40.0	29.0	41.0
32	35.32995235603365	36.0	34.0	40.0	29.0	41.0
33	35.14844172434198	36.0	34.0	40.0	27.0	41.0
34	34.99277158211942	36.0	34.0	40.0	27.0	41.0
35	34.833314873914915	36.0	34.0	40.0	26.0	41.0
36	34.64865763731474	36.0	33.0	40.0	25.0	41.0
37	34.559821582461645	36.0	33.0	40.0	25.0	41.0
38	34.531505560536836	36.0	33.0	40.0	25.0	41.0
39	34.46705989376086	36.0	33.0	40.0	24.0	41.0
40	34.26892582984938	36.0	33.0	40.0	23.0	41.0
41	34.234438243600856	35.0	33.0	40.0	23.0	41.0
42	34.148859239052996	35.0	33.0	40.0	23.0	41.0
43	34.06024500833474	35.0	33.0	40.0	23.0	41.0
44	34.01643676394365	35.0	33.0	40.0	23.0	41.0
45	33.98305083163828	35.0	33.0	40.0	23.0	41.0
46	33.96021321250503	35.0	33.0	40.0	23.0	41.0
47	33.90891988135365	35.0	33.0	40.0	23.0	41.0
48	33.85519426256386	35.0	33.0	40.0	23.0	41.0
49	33.79008467895234	35.0	33.0	39.0	24.0	41.0
50	33.647457360299015	35.0	33.0	39.0	23.0	41.0
51	33.484227775119486	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	10.0
13	18.0
14	44.0
15	125.0
16	329.0
17	924.0
18	2032.0
19	3952.0
20	6644.0
21	10307.0
22	15211.0
23	22068.0
24	30522.0
25	41571.0
26	54094.0
27	62161.0
28	69400.0
29	78404.0
30	92719.0
31	111696.0
32	138970.0
33	178585.0
34	303971.0
35	472846.0
36	228853.0
37	272021.0
38	379919.0
39	636415.0
40	442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.254020056884045	23.61661696105291	28.98043654243063	14.148926439632412
2	32.02076497904026	25.702666058542906	29.334484039551274	12.942084922865558
3	29.12049997231087	25.336765125885975	31.55611652829362	13.986618373509533
4	26.110722910233093	27.669838575497053	31.197340101510207	15.022098412759648
5	23.808978619637877	32.26190928046224	29.40958690932713	14.519525190572754
6	22.411735461185756	40.230217984990624	26.847222593830367	10.510823959993255
7	84.84872714013622	5.66485328806835	7.616967897412093	1.8694516743833256
8	86.2265567978675	4.438940495753608	7.111314648668526	2.223188057710364
9	80.89655528585446	6.520602888753796	9.145656633661641	3.4371851917300975
10	51.187521350184085	22.174044522872773	14.9089463260261	11.72948780091704
11	44.76109260675403	21.928855742133955	20.08199092916623	13.22806072194578
12	39.95046446178247	21.65544894031531	24.17702623747067	14.217060360431551
13	22.03976780955356	38.1376043864556	24.212586544079535	15.61004125991131
14	16.907354667858023	40.25650710055011	27.113940449086538	15.722197782505326
15	15.742513513227625	23.819992047931436	45.51451688072333	14.922977558117612
16	17.27095335844229	18.387696320581608	44.912418355962714	19.428931965013387
17	17.13561885822482	19.557764186944546	30.037850104129788	33.26876685070085
18	21.602559595402735	23.33319229508023	33.87089648684082	21.19335162267621
19	29.644975605567442	23.366574805133876	26.52036644227066	20.468083147028025
20	32.06148977462295	21.340975117741014	26.037735614253744	20.5597994933823
21	23.320218849886974	27.34914247705069	27.328702300810946	22.00193637225139
22	23.2728984418799	25.043633709552875	25.16107916663815	26.522388681929083
23	21.68338695898089	29.582286176156362	25.09820306895091	23.63612379591184
24	20.44655407251067	23.17085311757799	38.13184878127394	18.250744028637403
25	18.42854556168173	25.31601383585263	34.286233401301324	21.969207201164313
26	18.11388507083128	33.82849167677268	28.000272535683195	20.057350716712847
27	19.548430773136445	34.02972007847534	27.92884080867186	18.49300833971635
28	16.558409436952477	29.2113140886637	36.23925024064652	17.991026233737305
29	17.285264592948046	24.7734624911877	35.727623607065766	22.213649308798487
30	19.48810580855675	30.669255548247836	30.009289857876997	19.833348785318417
31	28.22187889086688	27.127473899108285	24.85173872165831	19.79890848836652
32	29.52429456502869	26.41079216416355	26.561869022337348	17.50304424847041
33	28.25230581988129	27.86876473512705	25.23142199537187	18.647507449619788
34	20.552363873715176	28.380764705260127	27.51216610489886	23.554705316125837
35	22.022874330560896	25.373600999048612	30.51183196868453	22.091692701705963
36	29.529801279175473	26.896907466668825	25.15566578662945	18.417625467526253
37	21.849583947523815	33.54410255803983	27.52759734906159	17.078716145374763
38	21.17984928403383	32.87421233765305	24.243666812060514	21.702271566252616
39	22.187702418411963	31.99083583209562	26.47519271943945	19.34626903005297
40	24.83132965679793	26.583740322027666	26.306693488823857	22.278236532350544
41	18.309015642179315	25.251177721265684	28.243096851590632	28.19670978496437
42	22.30433897963387	26.512806377086097	24.689243987259267	26.493610656020767
43	23.125990508540383	26.65881208042416	26.239088461473848	23.97610894956161
44	20.731005414002237	30.494814044174422	27.611691407472577	21.16248913435076
45	18.576946841230544	36.22739680511023	23.05281654428487	22.142839809374358
46	21.02351460274813	33.57608505602226	25.265146730598474	20.135253610631132
47	22.647653050875192	27.39876512713043	26.943045642260206	23.010536179734174
48	23.276974032576103	24.100958914934644	30.937840086265634	21.68422696622362
49	21.466011751390212	26.14734100374021	30.894097486885	21.49254975798458
50	19.66519177987579	33.16995710985242	26.630905173137936	20.533945937133858
51	19.961372111386204	32.66414830421204	24.51894029664078	22.85553928776097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2426.0
1	4377.5
2	6329.0
3	16672.0
4	27015.0
5	20322.5
6	13630.0
7	13824.5
8	14019.0
9	14850.0
10	15681.0
11	16060.5
12	16440.0
13	16501.0
14	16562.0
15	16401.5
16	16241.0
17	15561.5
18	14882.0
19	15224.0
20	15566.0
21	15437.5
22	15309.0
23	15869.5
24	16430.0
25	17528.0
26	23629.0
27	28632.0
28	35369.5
29	42107.0
30	48276.5
31	54446.0
32	58481.5
33	62517.0
34	72600.0
35	82683.0
36	87632.0
37	92581.0
38	111962.0
39	131343.0
40	169386.5
41	207430.0
42	256151.0
43	304872.0
44	311573.0
45	318274.0
46	297916.5
47	277559.0
48	259309.0
49	241059.0
50	222251.0
51	203443.0
52	186129.5
53	168816.0
54	155640.0
55	142464.0
56	138164.0
57	133864.0
58	130840.0
59	127816.0
60	118334.5
61	108853.0
62	94631.5
63	80410.0
64	69653.5
65	58897.0
66	48303.0
67	37709.0
68	36960.5
69	36212.0
70	30114.0
71	24016.0
72	20301.0
73	16586.0
74	13219.0
75	8134.0
76	6416.0
77	4624.0
78	2832.0
79	2247.0
80	1662.0
81	1184.0
82	706.0
83	587.5
84	469.0
85	432.0
86	395.0
87	258.5
88	122.0
89	79.0
90	36.0
91	26.0
92	16.0
93	11.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3214258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.170099047604847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.04604512843692	12.294765320150402
2	7.309608714335862	2.217749763914211
3	2.545461039370606	1.1584468826721381
4	1.3001412448519856	0.7889308584112353
5	0.8032993259737778	0.6093065169948211
6	0.5558721099022527	0.5059580979010956
7	0.38113641347575256	0.4047314000153229
8	0.2931045082852593	0.3557139537589519
9	0.2251603736126991	0.3074134652370322
>10	2.418117729405585	8.892441084361312
>50	1.187832957060391	13.183680347116034
>100	1.9089770998547535	55.54188300771901
>500	0.021932751442835782	2.1398419798753525
>1k	0.0028967784924500096	0.8298275306865676
>5k	0.0	0.0
>10k+	4.1382549892142993E-4	0.7693097911864842
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14456	0.4497461000330403	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10053	0.3127626967094739	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3557	0.11066317638472083	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3532	0.10988539190071239	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3267	0.10164087637022291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.11113793603376E-5	0.0	0.0	0.02908913970191565	0.0
2	3.11113793603376E-5	0.0	0.0	0.07242729115086592	0.0
3	3.11113793603376E-5	0.0	0.0	0.10829871155333518	0.0
4	3.11113793603376E-5	0.0	0.0	0.1779882013204914	0.0
5	6.22227587206752E-5	0.0	0.0	0.27981574596687636	0.0
6	6.22227587206752E-5	0.0	0.0	0.46701291557802765	0.0
7	6.22227587206752E-5	0.0	0.0	0.5818139054176734	0.0
8	6.22227587206752E-5	0.0	0.0	0.8783986848597717	0.0
9	6.22227587206752E-5	0.0	0.0	1.0053020012705887	0.0
10	6.22227587206752E-5	0.0	0.0	1.1844413236274125	0.0
11	6.22227587206752E-5	0.0	0.0	1.3662873359885859	0.0
12	6.22227587206752E-5	0.0	0.0	1.5337598910852832	0.0
13	1.244455174413504E-4	0.0	0.0	1.6126583491430992	0.0
14	1.244455174413504E-4	0.0	0.0	1.6456675226444175	0.0
15	1.244455174413504E-4	0.0	0.0	1.6856456451224513	0.0
16	1.244455174413504E-4	0.0	0.0	1.759068500412848	0.0
17	1.244455174413504E-4	0.0	0.0	1.834918043293351	0.0
18	1.244455174413504E-4	0.0	0.0	1.9473545683016111	0.0
19	1.244455174413504E-4	0.0	0.0	2.002235041493247	0.0
20	1.5555689680168798E-4	0.0	0.0	2.0603510981383573	0.0
21	1.5555689680168798E-4	0.0	0.0	2.135982861363338	0.0
22	1.5555689680168798E-4	0.0	0.0	2.213045748038894	0.0
23	1.5555689680168798E-4	0.0	0.0	2.3028331888728286	0.0
24	1.5555689680168798E-4	0.0	0.0	2.3742338045048035	0.0
25	1.5555689680168798E-4	0.0	0.0	2.432069858735671	0.0
26	1.5555689680168798E-4	0.0	0.0	2.4960037433211646	0.0
27	1.5555689680168798E-4	0.0	0.0	2.5587553954909654	0.0
28	1.5555689680168798E-4	0.0	0.0	2.620169258348272	0.0
29	1.5555689680168798E-4	0.0	0.0	2.687960953974448	0.0
30	1.5555689680168798E-4	0.0	0.0	2.777872840325823	0.0
31	1.5555689680168798E-4	0.0	0.0	2.8612824483908885	0.0
32	1.5555689680168798E-4	0.0	0.0	2.9404609088629474	0.0
33	1.8666827616202556E-4	0.0	0.0	3.0165593427783333	0.0
34	1.8666827616202556E-4	0.0	0.0	3.098724495668985	0.0
35	1.8666827616202556E-4	0.0	0.0	3.20425429445925	0.0
36	1.8666827616202556E-4	0.0	0.0	3.2989262218527573	0.0
37	1.8666827616202556E-4	0.0	0.0	3.3892736675151776	0.0
38	1.8666827616202556E-4	0.0	0.0	3.478781105934869	0.0
39	1.8666827616202556E-4	0.0	0.0	3.5711819026350717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGT	50	2.1827873E-11	45.0	20
CACTACG	25	3.892376E-5	45.0	1
CGGTCTA	135	0.0	45.0	31
CAATCGT	35	1.2128476E-7	45.0	18
TATGTCG	30	2.1667256E-6	44.999996	1
CATACGA	1215	0.0	42.03704	18
ACATACG	1220	0.0	41.86475	17
TTACCGG	165	0.0	40.909092	2
TACGATA	45	1.9301297E-8	40.000004	33
TGTAACG	85	0.0	39.705883	1
AATACGG	165	0.0	39.545456	2
ATCGTAC	40	3.4608092E-7	39.375	29
TACGAAC	40	3.4608092E-7	39.375	16
TATAGCG	80	0.0	39.375	1
TACCGTA	40	3.4608092E-7	39.375	45
CGTTTTT	11650	0.0	39.263947	1
GCGATAT	265	0.0	39.056602	9
TACGGGA	785	0.0	38.980892	4
ACTTACG	145	0.0	38.793106	1
GACCGAT	2005	0.0	38.715714	9
>>END_MODULE