FastQCFastQC Report
Thu 26 May 2016
SRR1545387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545387_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7265536
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238980.32892273880412953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT225350.041.0960731
CGTTAGG11800.039.8516962
TACGCTA356.2528798E-638.5714320
TACGGGA23700.037.879754
AGGGCGA79350.037.8544436
GGGCGAT160700.037.6073467
CGTAAGG13300.037.3872152
TCGAACG1150.037.1739121
TAGGGAT113350.037.159245
AATACGG3700.037.0945932
TAAGGGA93700.037.0037354
ACGTAGG10000.036.8999982
TAGGGCG30950.036.7851375
CGAGGGA45600.036.6611864
AACGGGA28050.036.4171144
ACGGGAT30900.036.4077645
GCGAGAC27850.036.35547621
CACGACC27750.036.16216327
ACACGAC27600.036.1141326
GCGTTAG4550.036.09891
Produced by FastQC (version 0.11.5)