##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545387_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7265536
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188833969028575	31.0	31.0	33.0	30.0	34.0
2	31.558029854920544	31.0	31.0	34.0	30.0	34.0
3	31.709064410388994	31.0	31.0	34.0	30.0	34.0
4	35.501555700776926	37.0	35.0	37.0	33.0	37.0
5	35.412182253312075	37.0	35.0	37.0	33.0	37.0
6	35.468155549707554	37.0	35.0	37.0	33.0	37.0
7	35.8776135717998	37.0	35.0	37.0	35.0	37.0
8	35.9352947944928	37.0	35.0	37.0	35.0	37.0
9	37.81567127325499	39.0	37.0	39.0	35.0	39.0
10	37.090905061925234	39.0	37.0	39.0	33.0	39.0
11	36.7801524897819	39.0	37.0	39.0	32.0	39.0
12	36.28793622383813	38.0	35.0	39.0	32.0	39.0
13	36.079640786309504	38.0	35.0	39.0	31.0	39.0
14	37.11129048152814	39.0	35.0	41.0	31.0	41.0
15	37.28219652342236	39.0	35.0	41.0	32.0	41.0
16	37.32308270167542	39.0	35.0	41.0	32.0	41.0
17	37.24237165709454	39.0	35.0	41.0	32.0	41.0
18	37.16658853524365	39.0	35.0	41.0	32.0	41.0
19	37.14504862407949	39.0	35.0	41.0	32.0	41.0
20	37.03230085158204	39.0	35.0	41.0	32.0	41.0
21	36.92302618829498	39.0	35.0	41.0	31.0	41.0
22	36.8168345735263	38.0	35.0	41.0	31.0	41.0
23	36.72793844803742	38.0	35.0	41.0	31.0	41.0
24	36.64580686132448	38.0	35.0	41.0	31.0	41.0
25	36.53392192950389	38.0	35.0	40.0	31.0	41.0
26	36.39859289115077	38.0	35.0	40.0	30.0	41.0
27	36.27779437057362	38.0	35.0	40.0	30.0	41.0
28	36.20301888257109	38.0	35.0	40.0	30.0	41.0
29	36.31806256276206	38.0	35.0	40.0	30.0	41.0
30	36.2934427687097	38.0	35.0	40.0	30.0	41.0
31	36.190062371172615	38.0	35.0	40.0	30.0	41.0
32	36.06477595045981	38.0	35.0	40.0	30.0	41.0
33	35.868937818214654	38.0	35.0	40.0	29.0	41.0
34	35.72185121648286	38.0	35.0	40.0	29.0	41.0
35	35.590275927336954	38.0	34.0	40.0	28.0	41.0
36	35.465016070390405	38.0	34.0	40.0	27.0	41.0
37	35.38034523536873	38.0	34.0	40.0	27.0	41.0
38	35.32111643242838	38.0	34.0	40.0	27.0	41.0
39	35.26764095587717	38.0	34.0	40.0	27.0	41.0
40	35.155350961030265	38.0	34.0	40.0	26.0	41.0
41	35.091699222190904	38.0	34.0	40.0	26.0	41.0
42	35.03547446465065	37.0	34.0	40.0	26.0	41.0
43	34.924073598974665	37.0	34.0	40.0	26.0	41.0
44	34.81130380470209	37.0	34.0	40.0	25.0	41.0
45	34.76272032786019	37.0	34.0	40.0	25.0	41.0
46	34.762828922738805	37.0	34.0	40.0	25.0	41.0
47	34.74418982990381	37.0	34.0	40.0	25.0	41.0
48	34.67823447574962	37.0	34.0	40.0	25.0	41.0
49	34.63774056036609	37.0	34.0	40.0	25.0	41.0
50	34.529426321746946	36.0	33.0	40.0	24.0	41.0
51	34.30547890754378	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	6.0
11	7.0
12	17.0
13	30.0
14	79.0
15	174.0
16	521.0
17	1317.0
18	3170.0
19	6068.0
20	10677.0
21	16885.0
22	25780.0
23	38363.0
24	55886.0
25	79566.0
26	106120.0
27	125000.0
28	140214.0
29	160453.0
30	190532.0
31	231348.0
32	284157.0
33	363052.0
34	574509.0
35	788443.0
36	552526.0
37	703137.0
38	1029562.0
39	1777134.0
40	801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.32054235227793	24.39603905341602	28.506788762728586	13.776629831577464
2	31.31394572953737	25.905631738663192	29.271095759486983	13.509326772312463
3	29.444379602551003	25.878366028328813	30.75442747789014	13.922826891230047
4	25.628804261653926	28.122426205031537	30.863228810647968	15.38554072266657
5	23.428663762728586	32.480273994926186	29.740076988125857	14.350985254219372
6	22.059638820866073	41.29649897818963	26.303262415876823	10.340599785067475
7	89.61469601141609	3.208476291356894	5.643024822944928	1.5338028742820902
8	90.90352040097248	2.2158172500968956	5.339303253056622	1.5413590958740002
9	85.82022028381664	4.67804440030302	6.96379455005109	2.537940765829252
10	41.27128404566435	32.55945604013248	13.686602062119025	12.48265785208414
11	33.79044574275043	24.14913366336634	25.407967147915862	16.65245344596737
12	31.110203018744937	23.41970365297206	27.971866081181073	17.498227247101937
13	22.46033878298862	31.427481743948416	28.643227423276134	17.46895204978683
14	19.542907777210104	33.34864764278919	29.02099721204327	18.087447367957438
15	18.96658415841584	25.27546487967302	38.435801570593	17.322149391318135
16	20.866994534195413	22.617477912159544	37.255517005214756	19.260010548430287
17	20.837856972974876	22.86468610161728	29.116516661675064	27.18094026373278
18	23.308879069624044	24.25121835470914	31.582790313061555	20.857112262605266
19	27.067252849617702	25.64872020453825	26.813878563123218	20.470148382720836
20	29.155921875550543	24.594978264507947	26.587990204714423	19.661109655227087
21	24.860946253655612	26.748694108734718	28.210141688101192	20.180217949508474
22	24.3542114442761	24.336800478312956	27.201062660758957	24.107925416651984
23	22.034919378281245	28.61139219460202	27.27607433229978	22.077614094816955
24	21.47458907367605	25.33141395211585	33.65497053486487	19.539026439343225
25	20.822978511151828	26.994773682216977	31.332210039286846	20.85003776734435
26	21.07989004527677	30.04010991067968	27.484854524153484	21.395145519890068
27	20.857993133610513	29.23045182076037	28.83862938673761	21.07292565889151
28	19.13668860769529	27.60524481607413	33.25022407156196	20.007842504668616
29	19.824882844156303	25.488924698742117	32.63397222173285	22.052220235368733
30	21.72571989182904	27.488102735985343	29.939608034424438	20.846569337761178
31	25.245611610760722	26.781189990662767	26.485162278460944	21.48803612011557
32	25.961869846904616	26.711931507874986	27.35381119851309	19.972387446707305
33	24.281126678059266	27.032637371833268	27.46204822328318	21.224187726824283
34	20.638559908037067	27.561614174095343	29.141869230294915	22.65795668757267
35	21.30010779658927	26.981312872168	29.933070319932348	21.785509011310385
36	25.756282812444947	27.534995353405446	26.393414057820376	20.315307776329234
37	21.886919285789787	30.403221455375075	27.77662928103308	19.93322997780205
38	21.209144101687748	29.789598454952255	27.01580447746732	21.985452965892673
39	21.512163727493746	29.93909878087453	27.168663124097108	21.38007436753462
40	23.433536080476376	27.00782158398224	27.08251669250555	22.476125643035832
41	19.66360086853881	26.676971939854127	27.99852619269934	25.66090099890772
42	22.26116559053592	27.551938356647053	26.151559912441424	24.035336140375605
43	21.896856611817768	27.296774800923153	27.398708092561925	23.407660494697158
44	21.635967394559742	28.73578769687467	27.874873925337372	21.753370983228216
45	20.340385072759943	30.13586334167225	26.21828589108911	23.3054656944787
46	22.058606550156796	29.575271528487367	27.1364425143582	21.22967940699764
47	21.84050839470068	27.076075323279657	28.54249156566013	22.540924716359537
48	22.70553748546563	25.45751614196117	29.28754327278813	22.549403099785067
49	20.874674628272434	25.967265181811776	30.03838945949755	23.11967073041824
50	20.302989345865193	29.336968394348332	28.484092570733942	21.875949689052536
51	20.222265776399702	29.643332577252384	27.088242354039675	23.046159292308236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1437.0
1	4334.0
2	7231.0
3	28084.0
4	48937.0
5	36757.0
6	24577.0
7	24478.5
8	24380.0
9	24744.0
10	25108.0
11	25427.0
12	25746.0
13	25336.0
14	24926.0
15	24583.5
16	24241.0
17	23646.5
18	23052.0
19	23282.0
20	23512.0
21	27514.0
22	31516.0
23	33146.0
24	34776.0
25	41388.5
26	58340.5
27	68680.0
28	84338.5
29	99997.0
30	119112.0
31	138227.0
32	162315.5
33	186404.0
34	208573.5
35	230743.0
36	246117.5
37	261492.0
38	297704.0
39	333916.0
40	397016.0
41	460116.0
42	518438.0
43	576760.0
44	598357.0
45	619954.0
46	610931.5
47	601909.0
48	574439.0
49	546969.0
50	520309.5
51	493650.0
52	468914.0
53	444178.0
54	416420.0
55	388662.0
56	358715.0
57	328768.0
58	310349.0
59	291930.0
60	268845.0
61	245760.0
62	214887.0
63	184014.0
64	157003.0
65	129992.0
66	111760.5
67	93529.0
68	78144.5
69	62760.0
70	52445.5
71	42131.0
72	35120.5
73	28110.0
74	22875.0
75	14703.5
76	11767.0
77	8843.0
78	5919.0
79	4057.5
80	2196.0
81	1626.0
82	1056.0
83	772.5
84	489.0
85	327.5
86	166.0
87	131.5
88	97.0
89	70.0
90	43.0
91	41.0
92	39.0
93	29.5
94	20.0
95	14.5
96	9.0
97	4.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7265536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.506400510237423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.90423698797454	13.024249376781974
2	8.781564523933389	2.8990404227707387
3	2.73318409529025	1.3534509403521537
4	1.3888053693216695	0.9169671062716671
5	0.7423898947128815	0.6127092468441907
6	0.5009842027533654	0.4961667539969424
7	0.3833964385271966	0.442994661796997
8	0.2819856071001557	0.3723653895134096
9	0.22821543276693068	0.3390313802281308
>10	2.729512240702264	10.986988728118765
>50	1.428598509352546	17.29103495696929
>100	1.8889968227536622	49.75929154780812
>500	0.006774895689034947	0.745481013779055
>1k	0.0011856067317522819	0.330692746669099
>5k	8.4686195125163E-5	0.09547412857716304
>10k+	8.4686195125163E-5	0.33406159952226094
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23898	0.32892273880412953	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0349045135830309	0.0
2	0.0	0.0	0.0	0.1161235729889715	0.0
3	0.0	0.0	0.0	0.18604270903068953	0.0
4	0.0	0.0	0.0	0.3037903879355907	0.0
5	0.0	0.0	0.0	0.5549349696980375	0.0
6	0.0	0.0	0.0	0.9137109774144674	0.0
7	0.0	0.0	0.0	1.1085899237165708	0.0
8	0.0	0.0	0.0	1.5448963435044571	0.0
9	0.0	0.0	0.0	1.7546675152390685	0.0
10	0.0	0.0	0.0	2.0396155218279834	0.0
11	0.0	0.0	0.0	2.3393594085832072	0.0
12	0.0	0.0	0.0	2.5757218737888024	0.0
13	0.0	0.0	0.0	2.687055710686727	0.0
14	0.0	0.0	0.0	2.7375956846129452	0.0
15	0.0	0.0	0.0	2.80511995260914	0.0
16	0.0	0.0	0.0	2.9442700442197243	0.0
17	0.0	0.0	0.0	3.119673483140129	0.0
18	1.3763609457031112E-5	0.0	0.0	3.3214755249991192	0.0
19	1.3763609457031112E-5	0.0	0.0	3.4405445104118955	0.0
20	1.3763609457031112E-5	0.0	0.0	3.5652841029209683	0.0
21	1.3763609457031112E-5	0.0	0.0	3.735539951904443	0.0
22	1.3763609457031112E-5	0.0	0.0	3.926702723653148	0.0
23	1.3763609457031112E-5	0.0	0.0	4.139267908107537	0.0
24	2.7527218914062224E-5	0.0	0.0	4.303385187273176	0.0
25	2.7527218914062224E-5	0.0	0.0	4.442039788943307	0.0
26	2.7527218914062224E-5	0.0	0.0	4.574115385292978	0.0
27	2.7527218914062224E-5	0.0	0.0	4.710044792290617	0.0
28	2.7527218914062224E-5	0.0	0.0	4.855195817624467	0.0
29	2.7527218914062224E-5	0.0	0.0	5.016973283182411	0.0
30	2.7527218914062224E-5	0.0	0.0	5.216394220605334	0.0
31	2.7527218914062224E-5	0.0	0.0	5.3951559802332545	0.0
32	2.7527218914062224E-5	0.0	0.0	5.568398532468906	0.0
33	2.7527218914062224E-5	0.0	0.0	5.740994195060075	0.0
34	2.7527218914062224E-5	0.0	0.0	5.919673373031253	0.0
35	2.7527218914062224E-5	0.0	0.0	6.130077670800888	0.0
36	2.7527218914062224E-5	0.0	0.0	6.318446429829816	0.0
37	4.129082837109334E-5	0.0	0.0	6.509760601282548	0.0
38	4.129082837109334E-5	0.0	0.0	6.696615363271203	0.0
39	5.505443782812445E-5	0.0	0.0	6.894205740636341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22535	0.0	41.096073	1
CGTTAGG	1180	0.0	39.851696	2
TACGCTA	35	6.2528798E-6	38.57143	20
TACGGGA	2370	0.0	37.87975	4
AGGGCGA	7935	0.0	37.854443	6
GGGCGAT	16070	0.0	37.607346	7
CGTAAGG	1330	0.0	37.387215	2
TCGAACG	115	0.0	37.173912	1
TAGGGAT	11335	0.0	37.15924	5
AATACGG	370	0.0	37.094593	2
TAAGGGA	9370	0.0	37.003735	4
ACGTAGG	1000	0.0	36.899998	2
TAGGGCG	3095	0.0	36.785137	5
CGAGGGA	4560	0.0	36.661186	4
AACGGGA	2805	0.0	36.417114	4
ACGGGAT	3090	0.0	36.407764	5
GCGAGAC	2785	0.0	36.355476	21
CACGACC	2775	0.0	36.162163	27
ACACGAC	2760	0.0	36.11413	26
GCGTTAG	455	0.0	36.0989	1
>>END_MODULE