FastQCFastQC Report
Thu 26 May 2016
SRR1545386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545386_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1360443
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27150.19956734681276617No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.1808969578291777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG302.165796E-645.0000041
ACGACCG302.165796E-645.00000419
CTTAACG253.891185E-545.01
TACCCGC207.033744E-445.042
ACTTCGC207.033744E-445.032
TAGTTCG207.033744E-445.01
GTTACGC253.891185E-545.030
ACGTCGC253.891185E-545.020
TATAGCG800.042.18751
CGTTTTT27300.041.3736231
AAATGCG603.6379788E-1241.2500041
ACGGGAT2450.040.4081655
TATTGCG900.040.0000041
AGGGCGA16050.039.532716
ATGACGG1600.039.3752
TCGTTAG750.039.0000041
TGGGCGA6950.038.8489236
GGGCGAT29450.038.6587457
CAACGCG356.2491363E-638.5714261
CCCGTTG356.2491363E-638.5714261
Produced by FastQC (version 0.11.5)