##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545386_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1360443
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322752956206177	31.0	31.0	33.0	30.0	34.0
2	31.702769612545325	31.0	31.0	34.0	30.0	34.0
3	31.875392794846974	31.0	31.0	34.0	30.0	34.0
4	35.63498139944121	37.0	35.0	37.0	33.0	37.0
5	35.55324552370074	37.0	35.0	37.0	33.0	37.0
6	35.620719868454614	37.0	35.0	37.0	33.0	37.0
7	35.968327228704176	37.0	35.0	37.0	35.0	37.0
8	36.02806218268608	37.0	35.0	37.0	35.0	37.0
9	37.9765826278646	39.0	38.0	39.0	35.0	39.0
10	37.2820353370189	39.0	37.0	39.0	34.0	39.0
11	36.91157365652218	39.0	37.0	39.0	33.0	39.0
12	35.92195409877518	37.0	35.0	39.0	32.0	39.0
13	35.46676928030061	37.0	35.0	39.0	31.0	39.0
14	36.392448636216294	38.0	35.0	41.0	31.0	41.0
15	36.751831572509836	38.0	35.0	41.0	32.0	41.0
16	36.92316914416848	38.0	35.0	41.0	32.0	41.0
17	36.90135933662785	38.0	35.0	41.0	32.0	41.0
18	36.860411645324355	38.0	35.0	40.0	32.0	41.0
19	36.830131067600774	38.0	35.0	40.0	32.0	41.0
20	36.692547942104156	38.0	35.0	40.0	32.0	41.0
21	36.564333088560126	38.0	35.0	40.0	32.0	41.0
22	36.45608526046295	38.0	35.0	40.0	31.0	41.0
23	36.39152393742332	37.0	35.0	40.0	31.0	41.0
24	36.31047019242997	37.0	35.0	40.0	31.0	41.0
25	36.22754426315546	37.0	35.0	40.0	31.0	41.0
26	36.090109618705085	37.0	35.0	40.0	31.0	41.0
27	35.97792630782767	37.0	35.0	40.0	31.0	41.0
28	35.968341929797866	37.0	35.0	40.0	31.0	41.0
29	36.116392969054935	37.0	35.0	40.0	31.0	41.0
30	36.130288442808705	37.0	35.0	40.0	31.0	41.0
31	35.99289643153002	37.0	35.0	40.0	31.0	41.0
32	35.86451986595543	37.0	35.0	40.0	30.0	41.0
33	35.71318019204039	37.0	34.0	40.0	30.0	41.0
34	35.60926698141708	37.0	34.0	40.0	30.0	41.0
35	35.50704586667725	36.0	34.0	40.0	30.0	41.0
36	35.32555792488182	36.0	34.0	40.0	29.0	41.0
37	35.210813683483984	36.0	34.0	40.0	28.0	41.0
38	35.20402471841893	36.0	34.0	40.0	29.0	41.0
39	35.190823871341905	36.0	34.0	40.0	29.0	41.0
40	35.05996502609812	36.0	34.0	40.0	28.0	41.0
41	35.06280380728924	36.0	34.0	40.0	28.0	41.0
42	35.036294795151285	36.0	34.0	40.0	28.0	41.0
43	34.91912781351369	36.0	34.0	40.0	28.0	41.0
44	34.809474560859954	35.0	34.0	40.0	27.0	41.0
45	34.786478375058714	35.0	34.0	40.0	27.0	41.0
46	34.82627864599987	35.0	34.0	40.0	27.0	41.0
47	34.81810189769068	35.0	34.0	40.0	28.0	41.0
48	34.80581472358636	35.0	34.0	40.0	28.0	41.0
49	34.81677365387598	36.0	34.0	40.0	28.0	41.0
50	34.68637274770057	35.0	34.0	40.0	27.0	41.0
51	34.45473202478898	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	7.0
15	12.0
16	70.0
17	203.0
18	430.0
19	828.0
20	1524.0
21	2409.0
22	3827.0
23	5826.0
24	8738.0
25	12737.0
26	17246.0
27	20548.0
28	23289.0
29	27042.0
30	33802.0
31	42641.0
32	55339.0
33	75111.0
34	140773.0
35	223859.0
36	92194.0
37	112463.0
38	165135.0
39	294243.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.557073688497056	24.764359844550636	30.79945282529294	12.879113641659371
2	29.082659104424074	25.91053061392502	31.117363976293017	13.889446305357886
3	28.405600234629457	25.249863463592376	32.2315598668963	14.112976434881874
4	25.13857618437524	27.740081723379813	31.907253740142	15.214088352102955
5	22.182259749214044	32.719121639054336	31.02783431573392	14.0707842959977
6	20.606229000406483	41.35167735803705	27.381889575674982	10.660204065881482
7	90.31139121594951	3.4022006067141364	4.913473037826649	1.3729351395097038
8	92.30765272782469	2.133275705046077	4.108735169352924	1.4503363977763126
9	88.8524546783658	3.431676299558305	5.680796622864758	2.0350723992111392
10	53.97197824532156	18.984845377571865	14.957848289123469	12.085328087983104
11	48.0736054358764	21.71572054102965	16.548139098808257	13.662534924285694
12	41.13226353474567	21.572825910383603	22.93348563666394	14.361424918206788
13	21.16736974647229	42.072251465147744	23.033673590146737	13.72670519823322
14	16.798939757123232	43.12734895912582	25.828204489272977	14.245506794477974
15	14.196699163434264	24.260406352930627	46.80637115998245	14.736523323652664
16	15.059726868380373	18.399594837857965	46.86355841442824	19.677119879333425
17	16.392454516653768	19.583106385199528	27.85107498072319	36.17336411742352
18	21.712265784013002	23.642445879761226	32.842022782284886	21.803265553940886
19	28.963065707273294	25.373793683381074	23.922795736388807	21.740344872956825
20	30.848701489147285	24.010928793047558	23.85002532263388	21.290344395171278
21	22.42321067475815	28.291225725737867	26.94056274316528	22.345000856338707
22	23.474926917188004	25.67082928134439	24.37529540010129	26.47894840136632
23	20.07346136515826	31.74620325879144	23.64817930629949	24.532156069750812
24	20.36498405298862	25.61614121282553	36.5965350992287	17.422339634957144
25	17.19719238512749	26.93990119394932	34.529561326714905	21.333345094208283
26	17.71408283919282	34.58520496632347	26.89351924336411	20.8071929511196
27	18.680238716359305	33.10289368977605	27.71207613990443	20.504791453960216
28	15.952891815386606	28.695873329496347	37.3088765938742	18.042358261242843
29	15.878651292262887	25.626431978407034	36.55228480722823	21.942631922101842
30	18.774546232366955	29.4408512521289	31.60117696956065	20.18342554594349
31	27.31073628222572	27.31294144627889	24.554942764967	20.821379506528388
32	28.64427249065194	27.42474326377511	25.183708541996978	18.747275703575966
33	26.42646549690064	27.621664413724055	24.819047913069493	21.132822176305808
34	18.891199410780164	27.09124895346589	29.454670280195494	24.562881355558446
35	20.351606057732667	25.254347297167172	30.947713355135054	23.446333289965107
36	29.353453250154544	24.555604314182954	25.658847889988778	20.432094545673728
37	21.083647017919898	30.501829183582114	28.72490798952988	19.689615808968107
38	20.965303213732586	32.46214652139046	23.576952507381787	22.99559775749517
39	20.642173174473317	32.291613834611226	26.046515730537774	21.019697260377686
40	22.710911078229664	26.281806735012054	24.50929586906618	26.497986317692106
41	17.20197024057605	23.99247892046929	26.87837711686561	31.92717372208905
42	21.12392801462465	27.78624315755971	23.662733389050477	27.427095438765164
43	21.9825453914644	28.735051744174505	25.130196560973157	24.152206303387942
44	20.586676545801623	31.796922032014574	26.47240641467522	21.143995007508583
45	17.67321379874056	35.32878628505568	22.886001104052138	24.11199881215163
46	21.540851031612497	32.00854427565139	26.050632036770377	20.39997265596574
47	21.306956631038567	27.112712550250173	28.412142221320558	23.168188597390703
48	23.75821699255316	23.233020420554187	29.609178774854954	23.399583812037697
49	20.3716730506166	23.381574972270062	31.15940910424031	25.087342872873027
50	18.66406751330265	32.44663686754976	26.473214974828053	22.416080644319532
51	17.69247223147166	33.54054524886379	23.42112091429042	25.345861605374132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	234.0
1	599.0
2	964.0
3	2835.5
4	4707.0
5	3767.0
6	2827.0
7	3010.0
8	3193.0
9	3575.0
10	3957.0
11	4064.5
12	4172.0
13	3968.5
14	3765.0
15	3765.0
16	3765.0
17	3759.5
18	3754.0
19	3517.5
20	3281.0
21	3457.5
22	3634.0
23	3819.5
24	4005.0
25	4967.0
26	7361.0
27	8793.0
28	10704.5
29	12616.0
30	14984.5
31	17353.0
32	21032.0
33	24711.0
34	30467.5
35	36224.0
36	39277.0
37	42330.0
38	52289.0
39	62248.0
40	81315.5
41	100383.0
42	120569.0
43	140755.0
44	146810.5
45	152866.0
46	143290.0
47	133714.0
48	123962.0
49	114210.0
50	106458.0
51	98706.0
52	92853.5
53	87001.0
54	77683.0
55	68365.0
56	61246.5
57	54128.0
58	47527.5
59	40927.0
60	38225.0
61	35523.0
62	31860.5
63	28198.0
64	24079.5
65	19961.0
66	16668.5
67	13376.0
68	10458.5
69	7541.0
70	6191.0
71	4841.0
72	4279.5
73	3718.0
74	2732.5
75	1361.5
76	976.0
77	757.0
78	538.0
79	430.5
80	323.0
81	214.0
82	105.0
83	72.0
84	39.0
85	27.5
86	16.0
87	17.0
88	18.0
89	11.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1360443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.07509052988902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76395543689956	15.611184453580144
2	7.216516960102112	2.897444625690588
3	2.393324431100345	1.4413861386520368
4	1.277505927880797	1.0258418861870748
5	0.7989318348208272	0.8019314455619222
6	0.6038959789146983	0.727395986840911
7	0.50449682981475	0.7089473671401182
8	0.39415421127462075	0.633014517926001
9	0.3670778353541849	0.6632208698625861
>10	5.736107988877172	29.046766123272366
>50	2.5834107289380497	36.52250667472123
>100	0.35840716763384634	9.33319588983496
>500	0.0011073341945432862	0.12130079529720722
>1k	0.0011073341945432862	0.4658632254328295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2715	0.19956734681276617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2461	0.1808969578291777	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04726401620648568	0.0
2	0.0	0.0	0.0	0.1790593211181946	0.0
3	0.0	0.0	0.0	0.2678539269928986	0.0
4	0.0	0.0	0.0	0.4343438130079687	0.0
5	0.0	0.0	0.0	0.7104303524660717	0.0
6	0.0	0.0	0.0	1.05994885489506	0.0
7	0.0	0.0	0.0	1.2385671432026186	0.0
8	0.0	0.0	0.0	1.715176600563199	0.0
9	0.0	0.0	0.0	1.8897520881065948	0.0
10	0.0	0.0	0.0	2.180392710315684	0.0
11	0.0	0.0	0.0	2.5546090501402854	0.0
12	0.0	0.0	0.0	2.8424564645486803	0.0
13	0.0	0.0	0.0	2.970355979633105	0.0
14	0.0	0.0	0.0	3.012548118517277	0.0
15	0.0	0.0	0.0	3.0839219283718613	0.0
16	0.0	0.0	0.0	3.259379481536529	0.0
17	0.0	0.0	0.0	3.472324823605252	0.0
18	0.0	0.0	0.0	3.7397377177875146	0.0
19	0.0	0.0	0.0	3.8832938976495157	0.0
20	0.0	0.0	0.0	4.021557683783885	0.0
21	0.0	0.0	0.0	4.23545859694232	0.0
22	0.0	0.0	0.0	4.453255299928038	0.0
23	0.0	0.0	0.0	4.716992920688335	0.0
24	0.0	0.0	0.0	4.906710534730231	0.0
25	0.0	0.0	0.0	5.059969436426223	0.0
26	0.0	0.0	0.0	5.219696819344875	0.0
27	0.0	0.0	0.0	5.364723108575663	0.0
28	0.0	0.0	0.0	5.530918972716975	0.0
29	0.0	0.0	0.0	5.7161527531840735	0.0
30	0.0	0.0	0.0	5.926966436668056	0.0
31	0.0	0.0	0.0	6.125945739733307	0.0
32	0.0	0.0	0.0	6.30956239989474	0.0
33	0.0	0.0	0.0	6.49457566395652	0.0
34	0.0	0.0	0.0	6.6966421966962235	0.0
35	0.0	0.0	0.0	6.942223966752007	0.0
36	0.0	0.0	0.0	7.158550560368939	0.0
37	0.0	0.0	0.0	7.365394948557198	0.0
38	0.0	0.0	0.0	7.566579415675629	0.0
39	0.0	0.0	0.0	7.772982771053252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACG	30	2.165796E-6	45.000004	1
ACGACCG	30	2.165796E-6	45.000004	19
CTTAACG	25	3.891185E-5	45.0	1
TACCCGC	20	7.033744E-4	45.0	42
ACTTCGC	20	7.033744E-4	45.0	32
TAGTTCG	20	7.033744E-4	45.0	1
GTTACGC	25	3.891185E-5	45.0	30
ACGTCGC	25	3.891185E-5	45.0	20
TATAGCG	80	0.0	42.1875	1
CGTTTTT	2730	0.0	41.373623	1
AAATGCG	60	3.6379788E-12	41.250004	1
ACGGGAT	245	0.0	40.408165	5
TATTGCG	90	0.0	40.000004	1
AGGGCGA	1605	0.0	39.53271	6
ATGACGG	160	0.0	39.375	2
TCGTTAG	75	0.0	39.000004	1
TGGGCGA	695	0.0	38.848923	6
GGGCGAT	2945	0.0	38.658745	7
CAACGCG	35	6.2491363E-6	38.571426	1
CCCGTTG	35	6.2491363E-6	38.571426	1
>>END_MODULE