FastQCFastQC Report
Thu 26 May 2016
SRR1545385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545385_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3854120
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC118900.3085010326611522No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79190.20546843377995494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCG1700.045.0000041
CCCGTAG207.035356E-445.01
TACGCAT351.2129203E-745.040
TACGACA207.035356E-445.019
CCGCGTA207.035356E-445.040
CAGTCGA351.2129203E-745.039
TCGACAC406.8230293E-945.014
TATCCGG1850.045.02
CACGCGA302.1668384E-644.99999628
ATATCGT302.1668384E-644.99999610
CTCGATT302.1668384E-644.99999637
ACGTTAG1150.043.043481
CGTTTTT85300.041.966591
CGGTCGA650.041.5384611
GTACCGA501.0822987E-940.59
GGCGTAT501.0822987E-940.58
CTTATCG451.9303116E-840.013
CTACGAT451.9303116E-840.020
CGACGAA403.4610457E-739.37531
CGTTCGA403.4610457E-739.37514