##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545385_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3854120
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20875504654759	31.0	31.0	33.0	30.0	34.0
2	31.556366174379626	31.0	31.0	34.0	30.0	34.0
3	31.707797889012276	31.0	31.0	34.0	30.0	34.0
4	35.48010025634905	37.0	35.0	37.0	33.0	37.0
5	35.37972481396532	37.0	35.0	37.0	33.0	37.0
6	35.43795911907257	37.0	35.0	37.0	33.0	37.0
7	35.905280063931585	37.0	35.0	37.0	35.0	37.0
8	35.980632933069025	37.0	35.0	37.0	35.0	37.0
9	37.87729494670638	39.0	38.0	39.0	35.0	39.0
10	37.152158209915626	39.0	37.0	39.0	33.0	39.0
11	36.74513896816913	39.0	35.0	39.0	32.0	39.0
12	35.6429784230901	37.0	35.0	39.0	31.0	39.0
13	35.11765565161438	37.0	35.0	39.0	30.0	39.0
14	35.99244548690752	38.0	35.0	40.0	30.0	41.0
15	36.39654214191567	38.0	35.0	40.0	31.0	41.0
16	36.60091849760776	38.0	35.0	40.0	32.0	41.0
17	36.55326637468475	38.0	35.0	40.0	32.0	41.0
18	36.520205390595	37.0	35.0	40.0	31.0	41.0
19	36.46852355401492	37.0	35.0	40.0	31.0	41.0
20	36.3134292653057	37.0	35.0	40.0	31.0	41.0
21	36.176486461241474	37.0	35.0	40.0	31.0	41.0
22	36.05426945710045	37.0	35.0	40.0	31.0	41.0
23	36.03982880657582	36.0	34.0	40.0	31.0	41.0
24	35.962403350181106	36.0	34.0	40.0	31.0	41.0
25	35.872844644172986	36.0	34.0	40.0	31.0	41.0
26	35.718773675962346	36.0	34.0	40.0	30.0	41.0
27	35.59136923603832	36.0	34.0	40.0	30.0	41.0
28	35.59982434381908	36.0	34.0	40.0	30.0	41.0
29	35.751875395680464	36.0	34.0	40.0	30.0	41.0
30	35.74118994738098	36.0	35.0	40.0	30.0	41.0
31	35.600899816300476	36.0	34.0	40.0	30.0	41.0
32	35.428535437402054	36.0	34.0	40.0	30.0	41.0
33	35.25539370855085	36.0	34.0	40.0	29.0	41.0
34	35.163433676169916	36.0	34.0	40.0	29.0	41.0
35	35.01785128641558	36.0	34.0	40.0	28.0	41.0
36	34.8438598175459	36.0	34.0	40.0	27.0	41.0
37	34.685209593889134	35.0	33.0	40.0	26.0	41.0
38	34.69244678422052	35.0	33.0	40.0	27.0	41.0
39	34.689697518499685	35.0	34.0	40.0	27.0	41.0
40	34.53517923676481	35.0	33.0	40.0	25.0	41.0
41	34.531235664691295	35.0	34.0	40.0	26.0	41.0
42	34.49507721607007	35.0	33.0	40.0	26.0	41.0
43	34.365492252446735	35.0	33.0	40.0	26.0	41.0
44	34.24477130966343	35.0	33.0	40.0	25.0	41.0
45	34.22045966394404	35.0	33.0	40.0	25.0	41.0
46	34.25828983010389	35.0	33.0	40.0	26.0	41.0
47	34.24603100059157	35.0	33.0	40.0	26.0	41.0
48	34.21886682303613	35.0	33.0	40.0	26.0	41.0
49	34.217346891119114	35.0	33.0	39.0	26.0	41.0
50	34.06028120556703	35.0	33.0	39.0	24.0	41.0
51	33.82476726204685	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	4.0
13	8.0
14	26.0
15	83.0
16	269.0
17	674.0
18	1588.0
19	3168.0
20	5676.0
21	9321.0
22	14638.0
23	21506.0
24	31167.0
25	44184.0
26	57727.0
27	68444.0
28	77670.0
29	90122.0
30	109811.0
31	136457.0
32	171936.0
33	227847.0
34	413613.0
35	650421.0
36	253929.0
37	305119.0
38	430648.0
39	727615.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.650171764241904	24.319663113758782	29.08217699500794	12.947988126991374
2	29.199194628086307	26.47802870694218	31.130270982740548	13.192505682230962
3	28.64550143742281	25.968080910817516	31.542920303467458	13.843497348292216
4	24.567631521592478	28.341099913858415	31.410023559204177	15.681245005344929
5	22.920796446400217	32.55420173736157	30.442617251149418	14.082384565088788
6	20.38434195095119	41.7368167052401	27.024586676076513	10.854254667732192
7	88.96736479403859	4.099509096758793	5.398819964090376	1.5343061451122435
8	91.1377953981713	2.3627702302990046	4.557668157711747	1.9417662138179403
9	86.89869542204187	3.665843305345967	6.9240968106857075	2.511364461926458
10	55.599125092109226	16.848489408736626	14.671286830716221	12.881098668437932
11	50.15632621713906	20.760692453789712	15.88964536651687	13.193335962554356
12	41.96607785953733	21.503663611926978	22.253484582732245	14.276773945803454
13	20.376428341618837	44.11710066111071	22.839143565846417	12.667327431424036
14	15.495132481604102	45.23009143462061	26.40921922514089	12.865556858634397
15	13.294007451765902	23.717139061575665	50.19119799072161	12.797655495936816
16	15.322486066858323	18.008832106940105	47.62378960696605	19.044892219235518
17	15.64024991437734	19.577205691571617	27.400651770053862	37.381892623997174
18	21.800436935020187	23.790385353855097	33.50173321017508	20.90744450094963
19	30.237797473872114	24.61801915871846	24.448979274127428	20.695204093281994
20	31.225960790011726	24.249115232530382	23.428616649196186	21.096307328261705
21	21.98644567372059	29.501416665801795	27.681312465621204	20.830825194856413
22	22.284360632258466	26.101807935404192	25.086945917615434	26.52688551472191
23	19.362085249032205	33.59661868338298	22.721555115045717	24.3197409525391
24	19.993098294811787	25.774703434246987	36.98763920168547	17.244559069255757
25	16.18112565254844	26.389967100142186	35.568534451444165	21.860372795865203
26	16.68432742104553	35.709682106421184	27.474313202494994	20.1316772700383
27	19.188608553963032	33.15737444604734	27.45077994457879	20.203237055410835
28	15.35748757174141	28.72409265928409	38.914797671063695	17.003622097910807
29	15.39020580573516	25.76902120328376	37.66387657882993	21.17689641215115
30	18.287961973161188	30.011442300706776	31.5178821624651	20.182713563666933
31	28.318345043745396	26.425046443805588	24.163362842879827	21.09324566956919
32	29.571134266706796	27.276187560325056	25.681608252986415	17.471069919981733
33	26.992127904683823	27.12826793146036	24.828131973057406	21.051472190798417
34	18.640441916702127	26.579763992818073	30.33206023683746	24.447733853642337
35	19.399421917324837	25.997400184737373	32.084211181800256	22.518966716137538
36	31.555374508318373	22.39123327763536	26.687181509657197	19.36621070438907
37	20.83863501914834	30.526709080153186	29.53883117287474	19.095824727823732
38	20.951345573049103	32.723916224715374	24.271480908741815	22.053257293493715
39	21.296612456280553	31.6209147613463	26.144774942139843	20.93769784023331
40	24.036304007140412	25.16525173061555	24.932358099903478	25.86608616234056
41	17.148557906863303	23.43180803918923	26.78152211140286	32.6381119425446
42	22.61496787852999	26.665153134827147	24.31356574263386	26.40631324400901
43	21.949835500710925	27.860860585555198	25.555172127489545	24.634131786244332
44	19.972912104449264	31.289632912312022	27.703237055410835	21.034217927827882
45	17.36155075607402	36.1677114360736	22.58580428217077	23.88493352568161
46	22.314510186501717	31.90479279316679	26.343653025852852	19.437043994478636
47	22.31567776820649	26.165064917542786	28.24426847114257	23.274988843108154
48	24.600635164447397	21.878457339159134	30.31478002760682	23.20612746878665
49	21.5324120681245	21.414252799601467	32.352287941216154	24.70104719105788
50	18.778917106888212	32.16420350170726	26.3549655952591	22.701913796145423
51	18.477188048114744	33.63351426525381	23.474437744543504	24.414859942087947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1255.0
1	1945.0
2	2635.0
3	8531.5
4	14428.0
5	11360.5
6	8293.0
7	9182.5
8	10072.0
9	11149.0
10	12226.0
11	12481.5
12	12737.0
13	12499.0
14	12261.0
15	12064.5
16	11868.0
17	11490.5
18	11113.0
19	10933.5
20	10754.0
21	11278.0
22	11802.0
23	12457.5
24	13113.0
25	15789.5
26	19654.5
27	20843.0
28	24915.0
29	28987.0
30	35002.0
31	41017.0
32	54208.5
33	67400.0
34	74543.5
35	81687.0
36	92667.0
37	103647.0
38	137762.5
39	171878.0
40	238074.0
41	304270.0
42	364473.0
43	424676.0
44	439923.5
45	455171.0
46	438614.0
47	422057.0
48	377411.5
49	332766.0
50	313526.5
51	294287.0
52	265569.0
53	236851.0
54	206693.0
55	176535.0
56	155801.0
57	135067.0
58	123644.5
59	112222.0
60	101062.0
61	89902.0
62	79554.0
63	69206.0
64	58024.0
65	46842.0
66	38975.5
67	31109.0
68	25533.0
69	19957.0
70	17231.5
71	14506.0
72	12286.5
73	10067.0
74	7906.5
75	4741.5
76	3737.0
77	2647.5
78	1558.0
79	1170.0
80	782.0
81	492.0
82	202.0
83	143.0
84	84.0
85	52.0
86	20.0
87	15.0
88	10.0
89	7.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3854120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.96454968631714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96492821004014	13.056753487230576
2	9.925253717083207	3.3675491966552387
3	3.7085489542428367	1.8874158899517621
4	1.8418347051092212	1.2498358547523465
5	1.0411611336655182	0.8831414891765482
6	0.6270894543304852	0.6382974123453014
7	0.4686846176093435	0.556571643785238
8	0.34965523780558927	0.47453949238671633
9	0.26060228949590797	0.3978900439669202
>10	2.332948231241391	8.860581412873032
>50	0.8005458871934868	9.918008581756194
>100	1.6526500270097648	55.00025662356573
>500	0.023009069472170702	2.406895317919396
>1k	0.0027796191308662593	0.7833229232999681
>5k	1.544232850481255E-4	0.2074557449454014
>10k+	1.544232850481255E-4	0.3114848853896732
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11890	0.3085010326611522	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7919	0.20546843377995494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0795771797453115	0.0
2	0.0	0.0	0.0	0.274226023061036	0.0
3	0.0	0.0	0.0	0.41630774340186605	0.0
4	0.0	0.0	0.0	0.7048042095212396	0.0
5	0.0	0.0	0.0	1.1464095565265222	0.0
6	0.0	0.0	0.0	1.760868888358432	0.0
7	0.0	0.0	0.0	2.057201125029838	0.0
8	0.0	0.0	0.0	2.8561383662158937	0.0
9	0.0	2.594626010606831E-5	0.0	3.1816601455066267	0.0
10	0.0	2.594626010606831E-5	0.0	3.7312278808132597	0.0
11	0.0	2.594626010606831E-5	0.0	4.302434797048353	0.0
12	0.0	2.594626010606831E-5	0.0	4.766691229126233	0.0
13	0.0	2.594626010606831E-5	0.0	4.968630971531764	0.0
14	0.0	2.594626010606831E-5	0.0	5.043978910879786	0.0
15	0.0	2.594626010606831E-5	0.0	5.1586354342885015	0.0
16	0.0	2.594626010606831E-5	0.0	5.4192656170539575	0.0
17	0.0	2.594626010606831E-5	0.0	5.733033740516642	0.0
18	0.0	2.594626010606831E-5	0.0	6.150483119363175	0.0
19	0.0	2.594626010606831E-5	0.0	6.346040081782612	0.0
20	0.0	2.594626010606831E-5	0.0	6.570345500399572	0.0
21	0.0	2.594626010606831E-5	0.0	6.847373719552063	0.0
22	0.0	2.594626010606831E-5	0.0	7.131355536412982	0.0
23	0.0	2.594626010606831E-5	0.0	7.470032069577491	0.0
24	0.0	2.594626010606831E-5	0.0	7.715250173839943	0.0
25	0.0	2.594626010606831E-5	0.0	7.920952123960852	0.0
26	0.0	2.594626010606831E-5	0.0	8.121776177181822	0.0
27	0.0	2.594626010606831E-5	0.0	8.310353595632726	0.0
28	0.0	2.594626010606831E-5	0.0	8.523190767282804	0.0
29	0.0	2.594626010606831E-5	0.0	8.75128433987525	0.0
30	0.0	2.594626010606831E-5	0.0	9.022163295382603	0.0
31	0.0	2.594626010606831E-5	0.0	9.28608865318153	0.0
32	2.594626010606831E-5	2.594626010606831E-5	0.0	9.516465496663312	0.0
33	2.594626010606831E-5	2.594626010606831E-5	0.0	9.751953753385987	0.0
34	2.594626010606831E-5	2.594626010606831E-5	0.0	9.998235654312788	0.0
35	2.594626010606831E-5	2.594626010606831E-5	0.0	10.296098720330452	0.0
36	2.594626010606831E-5	2.594626010606831E-5	0.0	10.558519195043226	0.0
37	2.594626010606831E-5	2.594626010606831E-5	0.0	10.81725530082094	0.0
38	2.594626010606831E-5	2.594626010606831E-5	0.0	11.06631345157909	0.0
39	2.594626010606831E-5	2.594626010606831E-5	0.0	11.312517513725572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCCG	170	0.0	45.000004	1
CCCGTAG	20	7.035356E-4	45.0	1
TACGCAT	35	1.2129203E-7	45.0	40
TACGACA	20	7.035356E-4	45.0	19
CCGCGTA	20	7.035356E-4	45.0	40
CAGTCGA	35	1.2129203E-7	45.0	39
TCGACAC	40	6.8230293E-9	45.0	14
TATCCGG	185	0.0	45.0	2
CACGCGA	30	2.1668384E-6	44.999996	28
ATATCGT	30	2.1668384E-6	44.999996	10
CTCGATT	30	2.1668384E-6	44.999996	37
ACGTTAG	115	0.0	43.04348	1
CGTTTTT	8530	0.0	41.96659	1
CGGTCGA	65	0.0	41.53846	11
GTACCGA	50	1.0822987E-9	40.5	9
GGCGTAT	50	1.0822987E-9	40.5	8
CTTATCG	45	1.9303116E-8	40.0	13
CTACGAT	45	1.9303116E-8	40.0	20
CGACGAA	40	3.4610457E-7	39.375	31
CGTTCGA	40	3.4610457E-7	39.375	14
>>END_MODULE