FastQCFastQC Report
Thu 26 May 2016
SRR1545384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545384_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3716193
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195980.5273676582459522No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50380.13556884693556012No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.035322E-445.00000424
CGTTTTT171600.042.3645131
CGTAAGG5100.040.147062
TATCGTG1500.039.01
GCGATAT3350.038.2835859
GGGCGAT84200.037.571267
TAATACG900.037.51
GTCGATA301.14041264E-437.49999615
CGCATCG601.5643309E-1037.49999621
GGCGATA18300.037.131158
ATAGGGA40950.037.0329674
ACGTTAG1950.036.923081
CATACGA15800.036.8829118
ATACTAT26400.036.8181845
CGAATAT4500.036.514
TATGGGC16100.036.3354034
AGGGATT31700.036.269726
TAAGGGC17950.036.2256284
TAAGGGA34800.036.142244
TAGGGTA17150.036.0787165
Produced by FastQC (version 0.11.5)