##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545384_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3716193
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255639306139376	31.0	31.0	33.0	30.0	34.0
2	31.62059209518989	31.0	31.0	34.0	30.0	34.0
3	31.736101704082646	31.0	31.0	34.0	30.0	34.0
4	35.57560331231451	37.0	35.0	37.0	33.0	37.0
5	35.51687170176576	37.0	35.0	37.0	33.0	37.0
6	35.58042545153064	37.0	35.0	37.0	33.0	37.0
7	35.891474958378105	37.0	35.0	37.0	35.0	37.0
8	35.93445980873437	37.0	35.0	37.0	35.0	37.0
9	37.84336335599362	39.0	38.0	39.0	35.0	39.0
10	37.22776669564794	39.0	37.0	39.0	34.0	39.0
11	36.909564438660745	39.0	37.0	39.0	33.0	39.0
12	36.38559515073625	38.0	35.0	39.0	32.0	39.0
13	36.19396732085766	38.0	35.0	39.0	32.0	39.0
14	37.23628051610883	39.0	35.0	41.0	32.0	41.0
15	37.39998729882974	39.0	35.0	41.0	32.0	41.0
16	37.442272239358935	39.0	35.0	41.0	32.0	41.0
17	37.355680665670484	39.0	35.0	41.0	32.0	41.0
18	37.25262681459225	39.0	35.0	41.0	32.0	41.0
19	37.196679505074144	39.0	35.0	41.0	32.0	41.0
20	37.041540361332146	39.0	35.0	41.0	32.0	41.0
21	36.94347171957969	38.0	35.0	41.0	32.0	41.0
22	36.832328676147874	38.0	35.0	41.0	31.0	41.0
23	36.72178005824778	38.0	35.0	41.0	31.0	41.0
24	36.62885915774557	38.0	35.0	40.0	31.0	41.0
25	36.524259907921895	38.0	35.0	40.0	31.0	41.0
26	36.376966158646766	38.0	35.0	40.0	31.0	41.0
27	36.25249603559342	38.0	35.0	40.0	30.0	41.0
28	36.154699446449634	38.0	35.0	40.0	30.0	41.0
29	36.2554356030486	38.0	35.0	40.0	30.0	41.0
30	36.20618896811872	38.0	35.0	40.0	30.0	41.0
31	36.06736275537896	38.0	35.0	40.0	30.0	41.0
32	35.878652427363164	38.0	35.0	40.0	30.0	41.0
33	35.62595780143819	38.0	34.0	40.0	29.0	41.0
34	35.38079857531619	38.0	34.0	40.0	27.0	41.0
35	35.1999078088786	38.0	34.0	40.0	26.0	41.0
36	35.04903324450587	38.0	34.0	40.0	25.0	41.0
37	34.94444287473767	38.0	34.0	40.0	25.0	41.0
38	34.86677817863604	37.0	34.0	40.0	25.0	41.0
39	34.78955075799346	37.0	34.0	40.0	24.0	41.0
40	34.64516105595162	37.0	34.0	40.0	24.0	41.0
41	34.564927601984074	37.0	33.0	40.0	24.0	41.0
42	34.47963709096917	37.0	33.0	40.0	24.0	41.0
43	34.355105345712666	37.0	33.0	40.0	23.0	41.0
44	34.223858933053265	36.0	33.0	40.0	23.0	41.0
45	34.204173195525634	36.0	33.0	40.0	23.0	41.0
46	34.206450795208966	36.0	33.0	40.0	23.0	41.0
47	34.1848717222168	36.0	33.0	40.0	23.0	41.0
48	34.0924330356362	36.0	33.0	40.0	23.0	41.0
49	34.03014133011929	36.0	33.0	40.0	23.0	41.0
50	33.92127238816714	36.0	33.0	40.0	23.0	41.0
51	33.69439046895573	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	8.0
12	12.0
13	22.0
14	54.0
15	104.0
16	283.0
17	751.0
18	1702.0
19	3222.0
20	5786.0
21	9315.0
22	14035.0
23	21168.0
24	31684.0
25	46752.0
26	62937.0
27	71149.0
28	75272.0
29	82883.0
30	97104.0
31	116872.0
32	144814.0
33	187872.0
34	306021.0
35	417700.0
36	295619.0
37	373727.0
38	520631.0
39	828284.0
40	402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.43787580462048	23.18194991487256	27.990069407051788	15.390104873455174
2	32.99497631043382	24.786064663487608	28.741079917001084	13.47787910907749
3	28.82638226808995	24.88638238110884	32.35714614391664	13.930089206884572
4	25.501205131165143	27.92731701502048	31.781018908329035	14.79045894548534
5	22.950261194722664	32.25833534480044	30.37928869679266	14.412114763684233
6	21.17465373838226	40.83644202548145	27.561162727554784	10.427741508581498
7	87.22305326983825	3.376170182764996	7.707511423653185	1.693265123743573
8	88.19170586672975	2.5886438083275007	7.426524940981268	1.7931253839614896
9	83.29295599017597	4.572394383176547	9.253179261679897	2.881470364967589
10	44.01652443777812	26.197105478644406	16.396430432972668	13.389939650604799
11	35.30500165088304	23.865418184685243	23.763459002263875	17.066121162167843
12	32.07629420753981	21.589755968002738	28.663096884365263	17.670852940092185
13	23.188488864814072	29.752087687587807	28.84387328645202	18.215550161146098
14	19.596936972864434	31.283278344262527	30.190870065144626	18.928914617728413
15	18.491074064237246	23.8488420811298	39.23477601943709	18.42530783519586
16	21.28751117070615	20.77537415306471	37.52660316619723	20.410511510031906
17	20.97283429574298	21.429618967583224	29.914135245397645	27.683411491276154
18	23.344508748603747	22.954755040978764	31.581648208260443	22.119088002157046
19	27.271188552370667	24.907559967956455	26.32869175524522	21.49255972442766
20	29.276735627024753	23.275943956624427	26.394888532430905	21.052431883919915
21	25.571303750908527	25.631042305929753	27.456216617382356	21.341437325779367
22	25.01796865770965	23.737599204347028	26.783592778954162	24.46083935898916
23	23.331969033901093	27.086133578099954	26.519290036873755	23.062607351125198
24	22.674764200890536	23.83907402010606	33.19394337161714	20.29221840738627
25	22.29601638020415	24.70991146046505	30.65588897024455	22.33818318908625
26	21.8353029565472	28.25935036205063	27.515094076114995	22.390252605287188
27	21.774514940424247	27.204372862227554	28.55400674830398	22.467105449044222
28	20.133023231032404	26.054728589177152	32.446431065340256	21.36581711445019
29	21.151646321921387	23.400776009211576	32.447695800514126	22.999881868352908
30	22.37717470540416	25.789161111922876	30.070504949554554	21.763159233118408
31	26.109569659056998	25.306435914388732	26.83988694882101	21.74410747773326
32	27.1037322334981	24.914152736416003	26.9621626218014	21.0199524082845
33	25.80675976732102	25.615784756066223	26.097164490649437	22.48029098596332
34	21.943101448175593	25.89504904616095	29.232389168162147	22.929460337501308
35	22.528808379973807	25.902502910909092	28.927211261632536	22.64147744748456
36	26.104752901692674	27.269627815347587	25.783698532342104	20.841920750617636
37	23.315366021086632	29.236721558864136	26.902235701967044	20.545676718082188
38	23.214913757170308	29.38622940197132	25.72578980693414	21.673067033924234
39	23.383311900108524	29.02505332742406	25.529836582760908	22.061798189706508
40	24.266581418134095	26.037318298592133	26.454761633747225	23.241338649526543
41	21.23702940078731	25.26273527774257	27.115733763020383	26.384501558449735
42	23.384468998246323	26.515549649870174	25.27070041841207	24.82928093347143
43	23.121242626526666	26.133895629209785	26.51450018876845	24.2303615554951
44	22.321714722566885	27.806898080912372	27.09234961693324	22.779037579587495
45	21.112520259308383	29.170820783527656	25.33875393447003	24.377905022693923
46	22.62325987912899	28.55145036869721	26.43753970797534	22.387750044198455
47	23.32432680433982	26.29389808333421	27.460791191415517	22.92098392091046
48	23.759826252296367	23.797310850109238	28.648162245609953	23.79470065198444
49	22.62511661800127	24.625820026032017	29.321593361808713	23.427469994158002
50	21.12446797031263	28.074779754442247	27.975995864585073	22.82475641066005
51	20.709715561059397	28.667429275067253	26.29801519996405	24.3248399639093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1022.0
1	2743.0
2	4464.0
3	21227.5
4	37991.0
5	27978.0
6	17965.0
7	18062.5
8	18160.0
9	18290.0
10	18420.0
11	18563.0
12	18706.0
13	18821.5
14	18937.0
15	18422.0
16	17907.0
17	17047.0
18	16187.0
19	15339.5
20	14492.0
21	14653.5
22	14815.0
23	15842.5
24	16870.0
25	18360.0
26	23819.0
27	27788.0
28	32145.5
29	36503.0
30	43602.5
31	50702.0
32	58821.5
33	66941.0
34	79859.5
35	92778.0
36	96851.5
37	100925.0
38	117380.5
39	133836.0
40	162640.0
41	191444.0
42	222905.5
43	254367.0
44	272216.0
45	290065.0
46	288557.0
47	287049.0
48	281052.5
49	275056.0
50	259762.5
51	244469.0
52	234186.0
53	223903.0
54	216995.0
55	210087.0
56	201690.5
57	193294.0
58	184178.5
59	175063.0
60	168583.5
61	162104.0
62	150384.5
63	138665.0
64	117290.5
65	95916.0
66	85674.0
67	75432.0
68	63411.0
69	51390.0
70	44122.5
71	36855.0
72	30938.5
73	25022.0
74	21799.5
75	14847.5
76	11118.0
77	8030.5
78	4943.0
79	4128.0
80	3313.0
81	2504.0
82	1695.0
83	1089.5
84	484.0
85	438.5
86	393.0
87	243.0
88	93.0
89	95.5
90	98.0
91	58.5
92	19.0
93	15.0
94	11.0
95	9.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3716193.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.083532985518822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65577782973135	15.796859085450496
2	7.1695130272694	2.8797830274654377
3	2.3789321198162137	1.4333208509591744
4	1.2517547192689507	1.005586287768673
5	0.8415859111918542	0.8451009203784758
6	0.6073926431736287	0.7319154110603421
7	0.5026688780582443	0.7066756895293542
8	0.4025008411108634	0.6466911135319278
9	0.35309146353003534	0.6382191649239523
>10	4.984110050348281	24.856339317134413
>50	2.1493864528620423	30.911618001481944
>100	0.6990697489372738	18.164244515616492
>500	0.0028562131851139726	0.3782670336075107
>1k	0.0010880812133767473	0.33243138352133095
>5k	1.3601015167209342E-4	0.13761621283323464
>10k+	1.3601015167209342E-4	0.5353319847371442
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19598	0.5273676582459522	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5038	0.13556884693556012	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034390033025733596	0.0
2	0.0	0.0	0.0	0.10763703607428354	0.0
3	0.0	0.0	0.0	0.19326229827137612	0.0
4	0.0	0.0	0.0	0.3134928675663508	0.0
5	0.0	0.0	0.0	0.5344986118858736	0.0
6	0.0	0.0	0.0	0.9859552504404373	0.0
7	0.0	0.0	0.0	1.238068098185428	0.0
8	0.0	0.0	0.0	1.8035661764606952	0.0
9	0.0	0.0	0.0	2.1281725680017156	0.0
10	0.0	0.0	0.0	2.5021843591008324	0.0
11	0.0	0.0	0.0	2.827113661750076	0.0
12	0.0	0.0	0.0	3.096125524158729	0.0
13	0.0	0.0	0.0	3.238340958071876	0.0
14	0.0	0.0	0.0	3.310457772241646	0.0
15	0.0	0.0	0.0	3.384027686398419	0.0
16	0.0	0.0	0.0	3.515533235222175	0.0
17	0.0	0.0	0.0	3.6634265227882405	0.0
18	0.0	0.0	0.0	3.8538095303446296	0.0
19	0.0	0.0	0.0	3.9641105830617516	0.0
20	0.0	0.0	0.0	4.082026956081129	0.0
21	0.0	0.0	0.0	4.237185743582209	0.0
22	0.0	0.0	0.0	4.411477014245492	0.0
23	0.0	0.0	0.0	4.600783651441139	0.0
24	0.0	0.0	0.0	4.7557002556110515	0.0
25	0.0	0.0	0.0	4.88771708035616	0.0
26	0.0	0.0	0.0	5.0113382162874744	0.0
27	0.0	0.0	0.0	5.138941922553538	0.0
28	0.0	0.0	0.0	5.27919298055833	0.0
29	0.0	0.0	0.0	5.4246644348127235	0.0
30	0.0	0.0	0.0	5.624465683025613	0.0
31	0.0	0.0	0.0	5.784710320481202	0.0
32	0.0	0.0	0.0	5.9511440875110635	0.0
33	0.0	0.0	0.0	6.114510199012807	0.0
34	0.0	0.0	0.0	6.2846843530462495	0.0
35	0.0	0.0	0.0	6.476601188366697	0.0
36	0.0	0.0	0.0	6.657054679345233	0.0
37	0.0	0.0	0.0	6.842782385091409	0.0
38	0.0	0.0	0.0	7.033945761159337	0.0
39	0.0	0.0	0.0	7.232374637162279	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.035322E-4	45.000004	24
CGTTTTT	17160	0.0	42.364513	1
CGTAAGG	510	0.0	40.14706	2
TATCGTG	150	0.0	39.0	1
GCGATAT	335	0.0	38.283585	9
GGGCGAT	8420	0.0	37.57126	7
TAATACG	90	0.0	37.5	1
GTCGATA	30	1.14041264E-4	37.499996	15
CGCATCG	60	1.5643309E-10	37.499996	21
GGCGATA	1830	0.0	37.13115	8
ATAGGGA	4095	0.0	37.032967	4
ACGTTAG	195	0.0	36.92308	1
CATACGA	1580	0.0	36.88291	18
ATACTAT	2640	0.0	36.81818	45
CGAATAT	450	0.0	36.5	14
TATGGGC	1610	0.0	36.335403	4
AGGGATT	3170	0.0	36.26972	6
TAAGGGC	1795	0.0	36.225628	4
TAAGGGA	3480	0.0	36.14224	4
TAGGGTA	1715	0.0	36.078716	5
>>END_MODULE