FastQCFastQC Report
Thu 26 May 2016
SRR1545383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545383_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5634292
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128340.22778372153945872No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC105020.18639431538159543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.0356345E-445.010
GTCGTTA1050.040.7142879
TATAGCG3600.040.01
GTATTCG356.2526306E-638.571431
CGCGAAA356.2526306E-638.5714326
TAGCATA21400.038.27102730
CGAATAT5000.038.2514
GTTGATC22500.037.816
GGGCGAT141350.037.6299977
TATCGGC301.1404875E-437.49999610
TGGGCGA38350.037.490226
AGGGATC30150.037.462696
CGTTTTT111850.037.4564171
GGCACCG31700.037.405368
ATAGGGC23900.037.3744774
TAGCCGT5000.037.3544
TAGGGCG30850.037.196115
GGACTAA33500.037.0746278
GGTACCT23950.037.0146148
GGCGATT39950.036.9461828
Produced by FastQC (version 0.11.5)