##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545383_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5634292
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238863019524015	31.0	31.0	33.0	30.0	34.0
2	31.60696800946774	31.0	31.0	34.0	30.0	34.0
3	31.7952819981641	31.0	31.0	34.0	30.0	34.0
4	35.58387389222994	37.0	35.0	37.0	33.0	37.0
5	35.497505809070596	37.0	35.0	37.0	33.0	37.0
6	35.54488922476861	37.0	35.0	37.0	33.0	37.0
7	35.86719271915619	37.0	35.0	37.0	35.0	37.0
8	35.90033352903967	37.0	35.0	37.0	35.0	37.0
9	37.794880528023754	39.0	37.0	39.0	35.0	39.0
10	37.19057088273025	39.0	37.0	39.0	33.0	39.0
11	36.81561410022768	39.0	35.0	39.0	32.0	39.0
12	36.05351018371075	37.0	35.0	39.0	32.0	39.0
13	35.71854831095016	37.0	35.0	39.0	31.0	39.0
14	36.700087961362314	38.0	35.0	41.0	31.0	41.0
15	36.958274260545956	38.0	35.0	41.0	32.0	41.0
16	37.058848387694496	38.0	35.0	41.0	32.0	41.0
17	36.98778267083069	38.0	35.0	41.0	32.0	41.0
18	36.943078030034656	38.0	35.0	41.0	32.0	41.0
19	36.90327054401866	38.0	35.0	41.0	32.0	41.0
20	36.77936127556044	38.0	35.0	41.0	31.0	41.0
21	36.665504556739336	38.0	35.0	41.0	31.0	41.0
22	36.530411806842814	38.0	35.0	40.0	31.0	41.0
23	36.44849113251497	38.0	35.0	40.0	31.0	41.0
24	36.33169331656932	38.0	35.0	40.0	31.0	41.0
25	36.18938670555236	38.0	35.0	40.0	30.0	41.0
26	36.01228814552032	38.0	35.0	40.0	30.0	41.0
27	35.8864663031309	37.0	34.0	40.0	30.0	41.0
28	35.810803202957885	37.0	34.0	40.0	30.0	41.0
29	35.947624475266814	37.0	35.0	40.0	30.0	41.0
30	35.955072083590984	37.0	35.0	40.0	30.0	41.0
31	35.83645647048467	37.0	35.0	40.0	30.0	41.0
32	35.720757816598784	37.0	34.0	40.0	30.0	41.0
33	35.54264954673986	37.0	34.0	40.0	29.0	41.0
34	35.4106961442538	37.0	34.0	40.0	29.0	41.0
35	35.28702506011403	37.0	34.0	40.0	28.0	41.0
36	35.134858825208205	37.0	34.0	40.0	27.0	41.0
37	35.0349804376486	37.0	34.0	40.0	27.0	41.0
38	34.9865855372778	36.0	34.0	40.0	27.0	41.0
39	34.917488834444505	36.0	34.0	40.0	27.0	41.0
40	34.729645357393615	36.0	33.0	40.0	26.0	41.0
41	34.6625052446696	36.0	34.0	40.0	26.0	41.0
42	34.59701999825355	36.0	33.0	40.0	26.0	41.0
43	34.46793794144855	35.0	33.0	40.0	26.0	41.0
44	34.353456121904934	35.0	33.0	40.0	25.0	41.0
45	34.314889608135324	35.0	33.0	40.0	25.0	41.0
46	34.31155538264613	35.0	33.0	40.0	25.0	41.0
47	34.27669581200264	35.0	33.0	40.0	25.0	41.0
48	34.195957362522215	35.0	33.0	40.0	25.0	41.0
49	34.157505858766285	35.0	33.0	39.0	25.0	41.0
50	34.03244649017126	35.0	33.0	39.0	24.0	41.0
51	33.76364732250299	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	14.0
12	14.0
13	21.0
14	69.0
15	180.0
16	463.0
17	1174.0
18	2607.0
19	5144.0
20	8766.0
21	13798.0
22	20539.0
23	29360.0
24	42069.0
25	58974.0
26	77501.0
27	94520.0
28	109833.0
29	128124.0
30	154961.0
31	191331.0
32	239055.0
33	312174.0
34	537991.0
35	777073.0
36	440868.0
37	541552.0
38	733920.0
39	1111503.0
40	686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75687664040131	24.011393090737933	29.134290519554185	13.097439749306567
2	30.57716923439538	25.722947976427207	30.488480185265516	13.211402603911903
3	29.92134592953294	25.654208195102417	30.07258054783103	14.351865327533611
4	26.66908282353843	28.005825754149765	29.52465012462968	15.800441297682122
5	24.163053672049656	32.79306432822438	27.971784209977045	15.072097789748916
6	22.911041884233192	41.55175131143363	24.7027488103208	10.834457994012379
7	88.41313158778424	4.3087578705541	5.2957851669739515	1.982325374687716
8	89.55524491808376	3.334704697591108	5.000202332431475	2.109848051893654
9	84.87350318371855	5.023736788934617	6.878929952512223	3.223830074834602
10	52.08508895172632	21.779648623110056	12.664146622148797	13.471115803014824
11	43.371270072619595	22.137848020656367	18.320598222456344	16.170283684267694
12	38.017962150346484	21.106751300784556	24.17375599276715	16.70153055610181
13	23.315759992559844	33.85190188935895	25.11232999638641	17.720008121694793
14	19.213984649712863	35.89389758287288	27.097743602922957	17.7943741644913
15	17.651552315712426	22.654807383074925	42.10646164593528	17.587178655277363
16	19.76278474740038	17.63728255475577	40.986711373851406	21.613221323992438
17	20.163917667028972	18.634231239701457	27.50893989874859	33.69291119452098
18	23.934400276024032	22.701077615430652	30.82314512630868	22.54137698223663
19	30.821263789665142	23.30883809358833	23.626943722476575	22.242954394269944
20	32.5042614049822	22.078159953371248	23.136429563820972	22.28114907782557
21	26.5240956627736	25.556431935015084	24.987984293323812	22.931488108887503
22	25.928155658244194	23.880817678600966	23.232768198737304	26.958258464417533
23	24.099673925313063	28.370077376181424	23.08231451263087	24.44793418587464
24	23.57849042967599	23.12125463146035	32.404319832908904	20.89593510595475
25	22.226679057457442	23.588553805873037	30.83780535336117	23.346961783308355
26	20.920853942252194	30.03314347215231	25.055339694854297	23.990662890741195
27	22.10629126072983	29.271770082203762	26.13618534502649	22.485753312039915
28	19.66925036899046	26.891683995078708	32.631642094516934	20.8074235414139
29	20.24641250400228	24.04891333285531	32.16972070315134	23.53495345999107
30	22.155472240345368	26.634544322516472	28.78303076943829	22.426952667699865
31	28.770393866700555	25.265871914341677	23.457907399900467	22.5058268190573
32	30.321644671593166	24.459204457276975	24.230284834367833	20.988866036762026
33	27.84591213944893	25.470298663967007	23.946203001193407	22.737586195390655
34	22.09061937151997	25.120884753576846	27.130187785794558	25.658308089108623
35	23.295757479378064	23.23970784616772	29.31835978681971	24.146174887634505
36	29.65126763043165	24.526098398876027	24.32184913383971	21.500784836852617
37	23.43930701497189	28.674676427845768	26.529207218937177	21.35680933824516
38	23.313843158998505	29.392299866602585	23.26173368366425	24.032123290734667
39	23.496918512565554	28.19635546045537	24.737553538226276	23.569172488752802
40	25.194611851852905	24.518289076959448	25.502600859167394	24.78449821202025
41	20.372586298331715	23.59572418326917	26.54960019821479	29.48208932018433
42	23.80396685155828	25.880483297635266	23.335212303515686	26.980337547290766
43	23.902896761474203	25.416201361235803	24.896437742310834	25.784464134979157
44	22.596539192501915	27.512720320494573	26.194879498613133	23.695860988390375
45	20.921989843621876	30.317846501388285	23.605432590288185	25.15473106470165
46	23.547111154338467	29.092563892677198	25.211526133185856	22.148798819798476
47	23.967927114888614	25.03432551951514	26.911384784459162	24.08636258113708
48	25.0635217344078	22.222295188108816	28.37259765734541	24.34158542013797
49	22.587735956886863	23.270785397703918	29.19003842896321	24.951440216446006
50	21.418219006043703	28.860786767884942	26.482972483499257	23.238021742572094
51	20.957770736766925	29.54232758969539	24.35390285061548	25.145998822922206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1564.0
1	4315.0
2	7066.0
3	16507.5
4	25949.0
5	19745.5
6	13542.0
7	13676.5
8	13811.0
9	14752.0
10	15693.0
11	15824.5
12	15956.0
13	15494.5
14	15033.0
15	15343.5
16	15654.0
17	14985.0
18	14316.0
19	14805.0
20	15294.0
21	14480.5
22	13667.0
23	15205.0
24	16743.0
25	19719.0
26	24237.0
27	25779.0
28	34544.0
29	43309.0
30	52909.0
31	62509.0
32	73148.0
33	83787.0
34	91092.0
35	98397.0
36	107204.0
37	116011.0
38	141493.5
39	166976.0
40	232867.5
41	298759.0
42	366233.0
43	433707.0
44	444016.0
45	454325.0
46	450173.5
47	446022.0
48	432288.0
49	418554.0
50	414898.0
51	411242.0
52	390666.0
53	370090.0
54	345423.5
55	320757.0
56	314251.0
57	307745.0
58	301340.0
59	294935.0
60	287486.5
61	280038.0
62	257562.5
63	235087.0
64	202152.5
65	169218.0
66	145753.5
67	122289.0
68	107225.0
69	92161.0
70	78833.5
71	65506.0
72	55121.0
73	44736.0
74	37424.5
75	23615.0
76	17117.0
77	13621.0
78	10125.0
79	7244.5
80	4364.0
81	3074.5
82	1785.0
83	1398.5
84	1012.0
85	749.0
86	486.0
87	333.5
88	181.0
89	136.0
90	91.0
91	79.5
92	68.0
93	44.5
94	21.0
95	11.5
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5634292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.131401296027827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94149885274891	11.94495498050864
2	9.223084403405563	2.7911638259013
3	3.131745362241972	1.4216308749917193
4	1.4598267593585048	0.8835689807413354
5	0.7992884546318108	0.604717717915793
6	0.5094378830015478	0.4625105425857176
7	0.39435077085965087	0.41769558356927056
8	0.27576852672216756	0.33382113941179925
9	0.20483371736766667	0.2789479058802578
>10	1.9828156932092957	6.928803659246919
>50	0.9362071567726514	10.53376132727083
>100	2.1165043296394996	59.843906373077346
>500	0.01930773402228874	1.8894238157455303
>1k	0.005093451306493334	1.2468304462180482
>5k	0.0	0.0
>10k+	2.369047119299225E-4	0.4182628269355467
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12834	0.22778372153945872	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10502	0.18639431538159543	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04321749742469861	0.0
2	0.0	0.0	0.0	0.15797903268059235	0.0
3	0.0	0.0	0.0	0.24033188198268746	0.0
4	1.77484589013136E-5	0.0	0.0	0.3935188307599251	0.0
5	1.77484589013136E-5	0.0	0.0	0.66489276736101	0.0
6	1.77484589013136E-5	0.0	0.0	1.0194892277503544	0.0
7	1.77484589013136E-5	0.0	0.0	1.2101254248093638	0.0
8	1.77484589013136E-5	0.0	0.0	1.6793059358655889	0.0
9	1.77484589013136E-5	0.0	0.0	1.8841054031278464	0.0
10	1.77484589013136E-5	0.0	0.0	2.1952358876678737	0.0
11	1.77484589013136E-5	0.0	0.0	2.538295849771364	0.0
12	1.77484589013136E-5	0.0	0.0	2.8159704892824156	0.0
13	1.77484589013136E-5	0.0	0.0	2.93641153138673	0.0
14	3.54969178026272E-5	0.0	0.0	2.984012898160053	0.0
15	3.54969178026272E-5	0.0	0.0	3.0572430395868726	0.0
16	5.3245376703940796E-5	0.0	0.0	3.2216647628486417	0.0
17	5.3245376703940796E-5	0.0	0.0	3.4054678032306454	0.0
18	5.3245376703940796E-5	0.0	0.0	3.636641480420255	0.0
19	5.3245376703940796E-5	0.0	0.0	3.750409101977675	0.0
20	5.3245376703940796E-5	0.0	0.0	3.881357231751567	0.0
21	5.3245376703940796E-5	0.0	0.0	4.048565463060842	0.0
22	5.3245376703940796E-5	0.0	0.0	4.225908064402768	0.0
23	5.3245376703940796E-5	0.0	0.0	4.43116899159646	0.0
24	1.597361301118224E-4	0.0	0.0	4.582403609894553	0.0
25	1.597361301118224E-4	0.0	0.0	4.7139019418943855	0.0
26	1.597361301118224E-4	0.0	0.0	4.836348559854548	0.0
27	1.597361301118224E-4	0.0	0.0	4.9548905168564215	1.77484589013136E-5
28	1.597361301118224E-4	0.0	0.0	5.086158118890537	1.77484589013136E-5
29	1.597361301118224E-4	0.0	0.0	5.22321171852648	1.77484589013136E-5
30	1.597361301118224E-4	0.0	0.0	5.397253106512761	1.77484589013136E-5
31	1.597361301118224E-4	0.0	0.0	5.561852314363544	1.77484589013136E-5
32	1.597361301118224E-4	0.0	0.0	5.715926686085847	1.77484589013136E-5
33	1.597361301118224E-4	0.0	0.0	5.864694268596658	1.77484589013136E-5
34	1.597361301118224E-4	0.0	0.0	6.022300583640322	1.77484589013136E-5
35	1.597361301118224E-4	0.0	0.0	6.217675619225982	1.77484589013136E-5
36	1.597361301118224E-4	0.0	0.0	6.390385872794665	1.77484589013136E-5
37	1.597361301118224E-4	0.0	0.0	6.562900893315433	1.77484589013136E-5
38	1.597361301118224E-4	0.0	0.0	6.729931640035696	1.77484589013136E-5
39	1.597361301118224E-4	0.0	0.0	6.8963589391533135	1.77484589013136E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.0356345E-4	45.0	10
GTCGTTA	105	0.0	40.714287	9
TATAGCG	360	0.0	40.0	1
GTATTCG	35	6.2526306E-6	38.57143	1
CGCGAAA	35	6.2526306E-6	38.57143	26
TAGCATA	2140	0.0	38.271027	30
CGAATAT	500	0.0	38.25	14
GTTGATC	2250	0.0	37.8	16
GGGCGAT	14135	0.0	37.629997	7
TATCGGC	30	1.1404875E-4	37.499996	10
TGGGCGA	3835	0.0	37.49022	6
AGGGATC	3015	0.0	37.46269	6
CGTTTTT	11185	0.0	37.456417	1
GGCACCG	3170	0.0	37.40536	8
ATAGGGC	2390	0.0	37.374477	4
TAGCCGT	500	0.0	37.35	44
TAGGGCG	3085	0.0	37.19611	5
GGACTAA	3350	0.0	37.074627	8
GGTACCT	2395	0.0	37.014614	8
GGCGATT	3995	0.0	36.946182	8
>>END_MODULE