FastQCFastQC Report
Thu 26 May 2016
SRR1545382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545382_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4121223
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109040.2645816545234267No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC64320.1560701762559318No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT44720.10851147826749485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1500.040.51
AAATGCG2000.039.3751
CATACGA14400.039.2187518
TCGATAG1900.039.0789451
TATAACG750.039.01
CGTTTTT83150.038.749251
CGTAAGG8150.038.6503032
CTATGCG2450.038.571431
GTATCGA356.2522213E-638.571438
GGCGATA19950.038.007528
AGGGCGA52500.037.5857166
CTATACG1200.037.4999961
ATACTAT32450.037.30354745
GGGCGAT98750.037.2987337
ACATACG15450.036.9902917
ATAGGGC22450.036.9821854
TAGTAGG20350.036.7076152
GTATGCG1850.036.4864851
ACGGGTA3950.036.4556965
CATATGC12600.036.24999633
Produced by FastQC (version 0.11.5)