##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545382_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4121223
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16582529021118	31.0	31.0	33.0	30.0	33.0
2	31.521102352384233	31.0	31.0	34.0	30.0	34.0
3	31.710997439352347	31.0	31.0	34.0	30.0	34.0
4	35.49734556950692	37.0	35.0	37.0	33.0	37.0
5	35.40594187696225	37.0	35.0	37.0	33.0	37.0
6	35.4600226680284	37.0	35.0	37.0	33.0	37.0
7	35.809411914861194	37.0	35.0	37.0	35.0	37.0
8	35.84485673306201	37.0	35.0	37.0	35.0	37.0
9	37.74539087062263	39.0	37.0	39.0	35.0	39.0
10	37.09087714981693	39.0	37.0	39.0	33.0	39.0
11	36.73899349780393	39.0	35.0	39.0	32.0	39.0
12	36.06755810107825	38.0	35.0	39.0	32.0	39.0
13	35.76940340282484	38.0	35.0	39.0	31.0	39.0
14	36.78016137442696	39.0	35.0	41.0	31.0	41.0
15	37.0190967584137	39.0	35.0	41.0	32.0	41.0
16	37.104113269289236	39.0	35.0	41.0	32.0	41.0
17	37.029115629025654	38.0	35.0	41.0	32.0	41.0
18	36.964651755073675	38.0	35.0	41.0	32.0	41.0
19	36.89165158012561	38.0	35.0	41.0	31.0	41.0
20	36.75292188750767	38.0	35.0	41.0	31.0	41.0
21	36.65038339347325	38.0	35.0	41.0	31.0	41.0
22	36.527926540252736	38.0	35.0	40.0	31.0	41.0
23	36.44687778360938	38.0	35.0	40.0	31.0	41.0
24	36.340399439680894	38.0	35.0	40.0	30.0	41.0
25	36.17396001138497	38.0	35.0	40.0	30.0	41.0
26	35.98490763542764	38.0	35.0	40.0	30.0	41.0
27	35.88391115938157	38.0	34.0	40.0	30.0	41.0
28	35.81365895512084	38.0	34.0	40.0	29.0	41.0
29	35.94875453233178	38.0	35.0	40.0	30.0	41.0
30	35.932699832064415	38.0	35.0	40.0	30.0	41.0
31	35.83160969450088	38.0	34.0	40.0	30.0	41.0
32	35.70835235074637	38.0	34.0	40.0	29.0	41.0
33	35.499000660726196	37.0	34.0	40.0	29.0	41.0
34	35.3521804085826	37.0	34.0	40.0	27.0	41.0
35	35.241277649862674	37.0	34.0	40.0	27.0	41.0
36	35.080247295523684	37.0	34.0	40.0	26.0	41.0
37	34.986936644777536	37.0	34.0	40.0	26.0	41.0
38	34.92976502363497	37.0	33.0	40.0	26.0	41.0
39	34.855546763667	37.0	33.0	40.0	26.0	41.0
40	34.708110189620896	36.0	33.0	40.0	25.0	41.0
41	34.644712018738126	36.0	33.0	40.0	25.0	41.0
42	34.55179688165382	36.0	33.0	40.0	25.0	41.0
43	34.42364341847068	36.0	33.0	40.0	25.0	41.0
44	34.295853682268586	35.0	33.0	40.0	24.0	41.0
45	34.24672724577146	35.0	33.0	40.0	24.0	41.0
46	34.25155178450669	35.0	33.0	40.0	24.0	41.0
47	34.218846687014995	35.0	33.0	40.0	24.0	41.0
48	34.12858464586847	35.0	33.0	40.0	24.0	41.0
49	34.07044219640626	35.0	33.0	39.0	24.0	41.0
50	33.93092511616091	35.0	33.0	39.0	24.0	41.0
51	33.68512041207185	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	9.0
12	12.0
13	20.0
14	53.0
15	135.0
16	334.0
17	987.0
18	2015.0
19	4050.0
20	7017.0
21	11134.0
22	16592.0
23	23814.0
24	33374.0
25	46088.0
26	60240.0
27	72696.0
28	83904.0
29	98289.0
30	117486.0
31	142105.0
32	175019.0
33	223962.0
34	371616.0
35	524659.0
36	323029.0
37	408064.0
38	551933.0
39	822148.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.924098987121056	23.406644095696834	28.721401389830152	12.947855527351953
2	30.193464415781428	25.47452540180427	30.60919052426913	13.722819658145166
3	30.061319176370706	25.31037509981867	30.56626637287038	14.062039350940243
4	26.337618711727078	28.155695530186065	29.915561472892875	15.591124285193983
5	23.65843828397541	33.06460242505683	28.39181475984192	14.885144531125832
6	22.468985541427873	41.40664069864698	25.313990531451463	10.810383228473684
7	89.15989258528354	3.8478383722501794	5.221823715921221	1.770445326545057
8	90.02878514460392	2.9523275008413763	5.042144043163886	1.976743311390818
9	85.55382710423581	4.416989811034249	6.966621316051085	3.0625617686788607
10	50.69672279320968	22.137797445078803	12.969887822134352	14.19559193957716
11	39.69588639100577	22.04386901655164	20.09553960074473	18.164704991697853
12	35.03045091226561	20.247242141471112	26.85164088427149	17.87066606199179
13	22.186108346963994	31.979997199860332	27.49341639605525	18.340478057120425
14	18.573515677263764	33.59170323954807	28.64957805001088	19.185203033177288
15	17.50487658639195	21.972822145270953	42.03858903048925	18.483712237847843
16	19.56506600103901	17.772612644353387	40.40074997154971	22.261571383057895
17	20.253623742272623	18.95570805074125	28.152808037808196	32.637860169177934
18	24.109469446326976	21.6071782575221	30.724932865802213	23.55841943034871
19	29.79591252402503	23.556041495449286	24.333990177187694	22.31405580333799
20	32.5883360352012	21.517010848478716	23.595714184842702	22.29893893147738
21	25.487118750914473	25.1517571361705	25.013909705929528	24.347214406985497
22	25.370575676200975	23.094212567483	23.416544069563816	28.118667686752207
23	23.75343435674313	27.559852985388076	23.348239102810016	25.338473555058776
24	24.035704935161238	23.239970270960832	32.38528951236077	20.339035281517162
25	22.714034159277478	23.936535343998614	30.516936356028296	22.832494140695616
26	22.036977858271683	28.207840245480533	26.384522264386078	23.370659631861706
27	21.871978293822004	27.4855061228184	27.39121372466377	23.25130185869583
28	20.03601843433369	25.26269507862108	33.188764597305216	21.51252188974001
29	20.45057984001351	22.67576881911025	32.0489815765854	24.824669764290842
30	22.51972290749615	26.387361227480287	28.199420414765232	22.89349545025833
31	28.437432286483894	25.245637035414003	23.440444741767188	22.876485936334916
32	29.94970182394886	24.653118746546838	24.538565372463466	20.858614057040832
33	28.427095549063957	25.24296792481261	24.078119529081537	22.2518169970419
34	22.760039920188742	25.251266432318758	26.761012447033323	25.227681200459184
35	23.33203032206702	24.170276638755052	28.329575953545827	24.168117085632105
36	28.95196401650675	25.240614254555023	25.09886021697928	20.70856151195895
37	24.036214492639687	28.748723376531675	27.17234665534964	20.042715475479003
38	23.62502587217435	28.79048282512254	24.362112897069633	23.222378405633474
39	23.37801181833645	27.96526662109767	25.82015096004269	22.836570600523196
40	24.8438388313372	24.688205418634226	25.6128095955982	24.855146154430372
41	20.484404750725695	23.14963300942463	26.32313272055407	30.042829519295605
42	23.12779483177688	25.63772938275847	23.617940596759748	27.616535188704905
43	23.518746740955294	25.72505782870764	25.49862019114229	25.257575239194775
44	22.76765901772362	26.996306678866926	26.407864849827344	23.828169453582106
45	20.760754756537075	30.437663771166957	23.89137884555143	24.91020262674454
46	22.98960769655027	28.38834491606011	26.38937519275225	22.232672194637367
47	23.869395080052694	25.128560138580227	27.559610338969765	23.442434442397317
48	24.46111263573944	22.834847810953203	28.422169826772297	24.28186972653506
49	21.982091238450334	23.793179840062038	29.601577007601872	24.623151913885756
50	21.60169444846833	28.56059960841721	27.21973550084526	22.6179704422692
51	21.918250965793405	28.99838227632914	24.40188264503037	24.681484112847084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1243.0
1	3099.0
2	4955.0
3	13062.5
4	21170.0
5	15777.0
6	10384.0
7	10524.0
8	10664.0
9	10842.0
10	11020.0
11	11041.5
12	11063.0
13	10841.0
14	10619.0
15	10752.5
16	10886.0
17	10245.0
18	9604.0
19	9788.5
20	9973.0
21	10003.0
22	10033.0
23	10880.0
24	11727.0
25	13718.5
26	19298.5
27	22887.0
28	29254.0
29	35621.0
30	40669.5
31	45718.0
32	54112.5
33	62507.0
34	75388.0
35	88269.0
36	93322.0
37	98375.0
38	117794.0
39	137213.0
40	177173.0
41	217133.0
42	259516.5
43	301900.0
44	311040.5
45	320181.0
46	315783.0
47	311385.0
48	303606.0
49	295827.0
50	284112.5
51	272398.0
52	273587.0
53	274776.0
54	263422.5
55	252069.0
56	239214.0
57	226359.0
58	220087.5
59	213816.0
60	208669.0
61	203522.0
62	185404.5
63	167287.0
64	149161.0
65	131035.0
66	114625.0
67	98215.0
68	84540.0
69	70865.0
70	60935.5
71	51006.0
72	41403.0
73	31800.0
74	25946.5
75	15507.5
76	10922.0
77	8622.5
78	6323.0
79	4370.5
80	2418.0
81	1913.0
82	1408.0
83	929.0
84	450.0
85	289.0
86	128.0
87	136.0
88	144.0
89	102.0
90	60.0
91	38.0
92	16.0
93	17.0
94	18.0
95	18.0
96	18.0
97	14.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4121223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.675949141383324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98030806877843	13.337475496671193
2	8.01342356552224	2.6726288765402297
3	2.5530069451382804	1.2772144192417312
4	1.126913343778277	0.7516939843037107
5	0.6682592011022985	0.5571928225421691
6	0.43967827434722784	0.4399231524951333
7	0.3332279597390792	0.38898247563670696
8	0.25346203965441627	0.3381376066038663
9	0.2087698592574466	0.313329199970784
>10	2.7617766994352135	11.658746985919914
>50	1.7720379375686064	21.947726537277216
>100	1.8820755123313715	44.85177071150415
>500	0.005001253620905926	0.5538784412215368
>1k	0.001765148336790327	0.4860540882553035
>5k	1.4709569473252726E-4	0.1577744080574369
>10k+	1.4709569473252726E-4	0.26747079375906274
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10904	0.2645816545234267	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6432	0.1560701762559318	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4472	0.10851147826749485	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04894178257279453	0.0
2	0.0	0.0	0.0	0.16189369029533224	0.0
3	0.0	0.0	0.0	0.2365317285669812	0.0
4	0.0	0.0	0.0	0.37202548855036477	0.0
5	0.0	0.0	0.0	0.6321909782605795	0.0
6	0.0	0.0	0.0	0.9464666192535566	0.0
7	0.0	0.0	0.0	1.118333077341362	0.0
8	0.0	0.0	0.0	1.5482297366582687	0.0
9	0.0	0.0	0.0	1.731136606779104	0.0
10	0.0	0.0	0.0	2.0056182351695115	0.0
11	0.0	0.0	0.0	2.2977402581709363	0.0
12	0.0	0.0	0.0	2.534466103872564	0.0
13	0.0	0.0	0.0	2.637275391309813	0.0
14	0.0	0.0	0.0	2.680951746605316	0.0
15	0.0	0.0	0.0	2.750008917255873	0.0
16	0.0	0.0	0.0	2.9037254232542136	0.0
17	0.0	0.0	0.0	3.084157299908304	0.0
18	0.0	0.0	0.0	3.3030728985060986	0.0
19	0.0	0.0	0.0	3.4189608278901678	0.0
20	0.0	0.0	0.0	3.540648006671806	0.0
21	0.0	0.0	0.0	3.704604191522759	0.0
22	0.0	0.0	0.0	3.8783147623897083	0.0
23	0.0	0.0	0.0	4.075804682250875	0.0
24	0.0	0.0	0.0	4.234325587331722	0.0
25	0.0	0.0	0.0	4.362127455854731	0.0
26	2.4264641830835167E-5	0.0	0.0	4.485513159564527	0.0
27	2.4264641830835167E-5	0.0	0.0	4.604337110610127	0.0
28	4.8529283661670334E-5	0.0	0.0	4.736239703602547	0.0
29	4.8529283661670334E-5	0.0	0.0	4.877023155505053	0.0
30	4.8529283661670334E-5	0.0	0.0	5.054785921557751	0.0
31	4.8529283661670334E-5	0.0	0.0	5.211802418845085	0.0
32	4.8529283661670334E-5	0.0	0.0	5.363747605989776	0.0
33	4.8529283661670334E-5	0.0	0.0	5.516930289867838	0.0
34	7.27939254925055E-5	0.0	0.0	5.684744552769894	0.0
35	7.27939254925055E-5	0.0	0.0	5.886626372802442	0.0
36	7.27939254925055E-5	0.0	0.0	6.059948709400098	0.0
37	7.27939254925055E-5	0.0	0.0	6.227762972302154	0.0
38	7.27939254925055E-5	0.0	0.0	6.39875590328405	0.0
39	7.27939254925055E-5	0.0	0.0	6.572005445956212	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	150	0.0	40.5	1
AAATGCG	200	0.0	39.375	1
CATACGA	1440	0.0	39.21875	18
TCGATAG	190	0.0	39.078945	1
TATAACG	75	0.0	39.0	1
CGTTTTT	8315	0.0	38.74925	1
CGTAAGG	815	0.0	38.650303	2
CTATGCG	245	0.0	38.57143	1
GTATCGA	35	6.2522213E-6	38.57143	8
GGCGATA	1995	0.0	38.00752	8
AGGGCGA	5250	0.0	37.585716	6
CTATACG	120	0.0	37.499996	1
ATACTAT	3245	0.0	37.303547	45
GGGCGAT	9875	0.0	37.298733	7
ACATACG	1545	0.0	36.99029	17
ATAGGGC	2245	0.0	36.982185	4
TAGTAGG	2035	0.0	36.707615	2
GTATGCG	185	0.0	36.486485	1
ACGGGTA	395	0.0	36.455696	5
CATATGC	1260	0.0	36.249996	33
>>END_MODULE