FastQCFastQC Report
Thu 26 May 2016
SRR1545381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545381_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences845132
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30640.36254691574807246No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9540.11288177468135156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG600.045.0000041
CTATGCG351.2114106E-745.01
TCCAACG207.0322264E-445.043
CGTTTTT34700.042.471181
TAGGGCG3550.041.8309825
CGTTAGG1850.041.3513532
TGGGCGA6400.039.7265626
TAGGGTA5400.039.5833365
GTGTACG403.456771E-739.3751
CGTATGG1500.039.02
ATTTGCG1100.038.8636361
GGGCGAT18750.038.47
AGGGCGA9150.038.1147546
ATAGGGC5100.037.9411774
GACCGAT5050.037.8712849
ACGGGTA1550.037.7419365
TTGGGCG3350.037.6119425
AAACCGT301.1396658E-437.50000417
TAGGCGA301.1396658E-437.5000045
TACAGCG301.1396658E-437.5000041
Produced by FastQC (version 0.11.5)