FastQCFastQC Report
Thu 26 May 2016
SRR1545380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545380_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences464720
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26930.579488724393183No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5010.10780685143742469No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4760.10242726803236357No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA207.028944E-445.06
ACCGCGA207.028944E-445.012
GCGTAAG253.8872065E-545.01
TCACGTA207.028944E-445.031
GCGATGT850.042.352949
TAGGGCG2350.042.127665
CGTTTTT19350.042.093021
AGGGCGA5450.041.6972476
CGGGTAT501.0786607E-940.56
TAGGGTA1850.040.1351365
CGTCATA1250.039.60000238
GGGCGAT10500.039.4285747
ACGTAGG403.4522964E-739.3752
CGTTAGG750.039.02
TACGGGA1100.038.8636364
ATTACGG356.240258E-638.571432
TGCGGGT1050.038.571434
TCTAGCG356.240258E-638.571431
CGATAAT356.240258E-638.5714310
ATACTAT3450.038.4782645
Produced by FastQC (version 0.11.5)