##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545380_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	464720
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.186839387157857	31.0	31.0	33.0	30.0	34.0
2	31.55438758822517	31.0	31.0	34.0	30.0	34.0
3	31.693604751248063	31.0	31.0	34.0	30.0	34.0
4	35.51809691857463	37.0	35.0	37.0	33.0	37.0
5	35.440288776037185	37.0	35.0	37.0	33.0	37.0
6	35.49160139438802	37.0	35.0	37.0	33.0	37.0
7	35.851844121191256	37.0	35.0	37.0	35.0	37.0
8	35.90354406954725	37.0	35.0	37.0	35.0	37.0
9	37.80601437424686	39.0	37.0	39.0	35.0	39.0
10	37.12961783439491	39.0	37.0	39.0	33.0	39.0
11	36.77604148734722	39.0	35.0	39.0	32.0	39.0
12	36.253322430710966	38.0	35.0	39.0	32.0	39.0
13	36.04503572043381	38.0	35.0	39.0	31.0	39.0
14	37.059224909623	39.0	35.0	41.0	31.0	41.0
15	37.24725641246342	39.0	35.0	41.0	32.0	41.0
16	37.29005422620072	39.0	35.0	41.0	32.0	41.0
17	37.188915906352214	39.0	35.0	41.0	32.0	41.0
18	37.110242726803236	39.0	35.0	41.0	32.0	41.0
19	37.06395463935273	38.0	35.0	41.0	32.0	41.0
20	36.927197021862625	38.0	35.0	41.0	32.0	41.0
21	36.823145119641936	38.0	35.0	41.0	31.0	41.0
22	36.71982484076433	38.0	35.0	41.0	31.0	41.0
23	36.64178860389052	38.0	35.0	41.0	31.0	41.0
24	36.554663022895504	38.0	35.0	40.0	31.0	41.0
25	36.446361249784815	38.0	35.0	40.0	31.0	41.0
26	36.28919134102255	38.0	35.0	40.0	30.0	41.0
27	36.178313823377515	38.0	35.0	40.0	30.0	41.0
28	36.105308572904114	38.0	35.0	40.0	30.0	41.0
29	36.18375150628335	38.0	35.0	40.0	30.0	41.0
30	36.13383757961783	38.0	35.0	40.0	30.0	41.0
31	36.02369168531589	38.0	35.0	40.0	30.0	41.0
32	35.86792046823894	38.0	35.0	40.0	29.0	41.0
33	35.64513900843519	38.0	34.0	40.0	29.0	41.0
34	35.44357677741436	38.0	34.0	40.0	27.0	41.0
35	35.30477061456361	38.0	34.0	40.0	27.0	41.0
36	35.16556851437425	38.0	34.0	40.0	26.0	41.0
37	35.07080392494405	38.0	34.0	40.0	26.0	41.0
38	35.0022099328628	38.0	34.0	40.0	26.0	41.0
39	34.950451885006025	37.0	34.0	40.0	25.0	41.0
40	34.804755551730075	37.0	34.0	40.0	24.0	41.0
41	34.729579101394386	37.0	34.0	40.0	24.0	41.0
42	34.67652134618695	37.0	33.0	40.0	24.0	41.0
43	34.557841280771214	37.0	33.0	40.0	24.0	41.0
44	34.43671458082286	37.0	33.0	40.0	23.0	41.0
45	34.41225899466345	37.0	33.0	40.0	23.0	41.0
46	34.42062962644173	36.0	33.0	40.0	23.0	41.0
47	34.40313522120847	36.0	33.0	40.0	23.0	41.0
48	34.31714796006197	36.0	33.0	40.0	23.0	41.0
49	34.27305259080737	36.0	33.0	40.0	24.0	41.0
50	34.182531416767084	36.0	33.0	40.0	24.0	41.0
51	33.92483646066449	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	2.0
13	2.0
14	3.0
15	10.0
16	36.0
17	91.0
18	224.0
19	402.0
20	799.0
21	1130.0
22	1862.0
23	2685.0
24	3868.0
25	5552.0
26	7578.0
27	8872.0
28	9477.0
29	10792.0
30	12570.0
31	14840.0
32	18451.0
33	23803.0
34	38135.0
35	51888.0
36	35206.0
37	44679.0
38	65028.0
39	106685.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.35346014804614	24.04652263728697	28.210320192804268	15.389697021862627
2	31.482398003098638	25.367963504906182	29.782234463763125	13.367404028232055
3	28.58818213117576	25.334394904458602	32.18669306248924	13.890729901876398
4	25.107161301428814	27.90153210535376	32.17744017903254	14.813866414184885
5	22.87506455500086	32.026166293682216	30.842657944568774	14.256111206748148
6	21.415475985539683	41.030297813737306	27.051557927354107	10.502668273368911
7	88.26820451024273	3.325443277672577	6.898347391977966	1.5080048201067309
8	89.4364348424858	2.3183852642451366	6.634962988466173	1.610216904802892
9	84.72370459631607	4.267300740230676	8.304355310724738	2.704639352728525
10	43.851136168015145	28.22861077638148	15.12674298502324	12.793510070580133
11	34.78718368049578	22.820838354277846	25.491478739886382	16.900499225339992
12	32.63427440179033	21.455069719400928	28.191384059218453	17.71927181959029
13	22.987390256498536	29.77169048028921	29.069977620933035	18.170941642279224
14	19.33207092442761	31.791616457221554	30.382380788431746	18.49393182991909
15	19.370158374935446	23.444009295920125	39.28343949044586	17.90239283869857
16	21.335642967808575	20.823291444310552	37.641590635221206	20.199474952659667
17	20.836632811155102	21.669392322258567	29.988810466517474	27.50516440006886
18	23.4857548631434	23.643914615252196	31.782363573764847	21.087966947839558
19	26.522206920296092	25.808443794112584	26.57750903770012	21.091840247891202
20	28.937639869168528	24.199517989326907	26.5581425374419	20.304699604062662
21	24.600834911344467	26.11981408159752	28.258090893441214	21.0212601136168
22	23.7941125839215	24.16616457221553	28.110259941470133	23.92946290239284
23	21.818944740919264	27.51570838354278	27.764029953520396	22.90131692201756
24	22.217033912893786	24.283654673782063	33.96819590290928	19.531115510414875
25	21.307453950766053	25.63328455844379	30.321053537614045	22.738207953176108
26	20.708168359442244	28.89524875193665	27.8434326045791	22.553150284042005
27	20.406050955414013	27.50688586675848	29.43299190910656	22.654071268720948
28	19.407169908762263	26.50778963677053	32.99728868996385	21.087751764503356
29	19.847435014632467	24.429979342399726	33.02052849027371	22.702057152694096
30	21.72275778963677	26.57428128765708	30.648347391977964	21.05461353072818
31	25.57755207436736	26.126484765019796	26.581167154415564	21.71479600619728
32	26.72275778963677	25.91646582888621	26.487132036495094	20.873644344981926
33	24.937596832501292	27.12794801170597	26.461525219487005	21.472929936305732
34	20.767989326906523	26.554054054054056	29.435574109140987	23.242382509898434
35	21.489068686520916	26.863918058185575	29.50034429333792	22.146668961955587
36	25.698485109313136	26.97624375968325	26.479385436391805	20.84588569461181
37	22.3853072818041	29.44611809261491	27.47159579962128	20.696978825959718
38	22.302461697366155	29.44267515923567	26.08172663108969	22.173136512308485
39	22.23769151316922	29.32905835772078	26.155749698743332	22.277500430366672
40	24.26213634016182	26.311972800826304	26.46496815286624	22.960922706145638
41	20.27995352039938	25.573463590979518	27.40661043208814	26.739972456532968
42	22.07651919435359	27.346359097951456	25.261017386813567	25.31610432088139
43	22.23317266310897	26.752237906696507	26.754174556722326	24.260414873472197
44	21.96118092614908	28.0194525735927	27.280943363745912	22.73842313651231
45	20.49190910655879	29.682604579101397	25.500731623343086	24.32475469099673
46	21.8839301084524	28.85759166810122	26.662291272163884	22.596186951282494
47	21.912979858839734	26.852943708039252	28.24130659321742	22.9927698399036
48	23.039894990531934	24.562747460836633	28.964968152866245	23.432389395765192
49	21.321656050955415	25.03959373386125	29.499913926665517	24.13883628851782
50	20.632423825098982	28.254002410053364	28.28886211051816	22.824711654329487
51	19.994405233258735	28.941297985883974	27.1303150284042	23.93398175245309
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	303.5
2	479.0
3	2701.0
4	4923.0
5	3462.5
6	2002.0
7	1984.0
8	1966.0
9	2052.0
10	2138.0
11	2123.0
12	2108.0
13	2056.5
14	2005.0
15	1946.5
16	1888.0
17	1829.0
18	1770.0
19	1684.5
20	1599.0
21	1621.5
22	1644.0
23	1858.5
24	2073.0
25	2330.5
26	3033.5
27	3479.0
28	4339.0
29	5199.0
30	5972.5
31	6746.0
32	8143.0
33	9540.0
34	11482.0
35	13424.0
36	14176.5
37	14929.0
38	16591.0
39	18253.0
40	22109.0
41	25965.0
42	29883.5
43	33802.0
44	35412.5
45	37023.0
46	36818.5
47	36614.0
48	35381.0
49	34148.0
50	33154.0
51	32160.0
52	31077.0
53	29994.0
54	28334.5
55	26675.0
56	25377.5
57	24080.0
58	22730.5
59	21381.0
60	20159.5
61	18938.0
62	16584.0
63	14230.0
64	12339.5
65	10449.0
66	8689.0
67	6929.0
68	5788.0
69	4647.0
70	4044.5
71	3442.0
72	2804.0
73	2166.0
74	1767.0
75	1116.0
76	864.0
77	657.0
78	450.0
79	355.0
80	260.0
81	203.0
82	146.0
83	103.0
84	60.0
85	47.0
86	34.0
87	22.5
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	464720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.45417912202019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.66586049305576	22.969174987513526
2	7.469005235606932	5.146768885018157
3	4.45926572572667	4.609210202006161
4	3.3625596150569357	4.634169251425717
5	2.7870229206842585	4.801229346321565
6	2.341170783732775	4.839787052278165
7	2.0754130589172326	5.005465729887993
8	1.7780817743734068	4.900987835828867
9	1.5213966205984204	4.717662451356076
>10	7.515725323872595	36.915729220442564
>50	0.017588629602562703	0.41727552158814996
>100	0.005653488086538012	0.3512646117933441
>500	6.281653429486679E-4	0.10843103543343524
>1k	6.281653429486679E-4	0.5828438691062696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2693	0.579488724393183	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	501	0.10780685143742469	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	476	0.10242726803236357	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03722671716302289	0.0
2	0.0	0.0	0.0	0.11404716818729557	0.0
3	0.0	0.0	0.0	0.18505766913410227	0.0
4	0.0	0.0	0.0	0.29673782062317094	0.0
5	0.0	0.0	0.0	0.5308572904114306	0.0
6	0.0	0.0	0.0	0.9780082630401101	0.0
7	0.0	0.0	0.0	1.2411774832156999	0.0
8	0.0	0.0	0.0	1.7683766569116888	0.0
9	0.0	0.0	0.0	2.088569461180926	0.0
10	0.0	0.0	0.0	2.4324324324324325	0.0
11	0.0	0.0	0.0	2.737777586503701	0.0
12	0.0	0.0	0.0	2.9647960061972802	0.0
13	0.0	0.0	0.0	3.083792391117232	0.0
14	0.0	0.0	0.0	3.1517903253572044	0.0
15	0.0	0.0	0.0	3.2101050094680668	0.0
16	0.0	0.0	0.0	3.321354794284731	0.0
17	0.0	0.0	0.0	3.4627302461697367	0.0
18	0.0	0.0	0.0	3.63380099845068	0.0
19	0.0	0.0	0.0	3.7454811499397485	0.0
20	0.0	0.0	0.0	3.8556550180754003	0.0
21	0.0	0.0	0.0	3.997676019969014	0.0
22	0.0	0.0	0.0	4.16724048889654	0.0
23	0.0	0.0	0.0	4.359399208125323	0.0
24	0.0	0.0	0.0	4.499268376656912	0.0
25	0.0	0.0	0.0	4.621922878292305	0.0
26	0.0	0.0	0.0	4.7435014632466865	0.0
27	0.0	0.0	0.0	4.872181098295748	0.0
28	0.0	0.0	0.0	5.001075916681012	0.0
29	0.0	0.0	0.0	5.14783095197108	0.0
30	0.0	0.0	0.0	5.333534171113789	0.0
31	0.0	0.0	0.0	5.492339473231193	0.0
32	0.0	0.0	0.0	5.6451196419349285	0.0
33	0.0	0.0	0.0	5.791229127216388	0.0
34	0.0	0.0	0.0	5.959932862799105	0.0
35	0.0	0.0	0.0	6.166724048889654	0.0
36	0.0	0.0	0.0	6.335642967808573	0.0
37	0.0	0.0	0.0	6.509295920123946	0.0
38	0.0	0.0	0.0	6.702960922706145	0.0
39	0.0	0.0	0.0	6.929764159063522	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	20	7.028944E-4	45.0	6
ACCGCGA	20	7.028944E-4	45.0	12
GCGTAAG	25	3.8872065E-5	45.0	1
TCACGTA	20	7.028944E-4	45.0	31
GCGATGT	85	0.0	42.35294	9
TAGGGCG	235	0.0	42.12766	5
CGTTTTT	1935	0.0	42.09302	1
AGGGCGA	545	0.0	41.697247	6
CGGGTAT	50	1.0786607E-9	40.5	6
TAGGGTA	185	0.0	40.135136	5
CGTCATA	125	0.0	39.600002	38
GGGCGAT	1050	0.0	39.428574	7
ACGTAGG	40	3.4522964E-7	39.375	2
CGTTAGG	75	0.0	39.0	2
TACGGGA	110	0.0	38.863636	4
ATTACGG	35	6.240258E-6	38.57143	2
TGCGGGT	105	0.0	38.57143	4
TCTAGCG	35	6.240258E-6	38.57143	1
CGATAAT	35	6.240258E-6	38.57143	10
ATACTAT	345	0.0	38.47826	45
>>END_MODULE