FastQCFastQC Report
Thu 26 May 2016
SRR1545379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545379_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6904211
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC159890.23158330473967265No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152370.22069140123324735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG1200.041.2499969
TCGTTAG2700.040.8333361
ATTACGC451.9306754E-840.016
TACGGGA19300.039.987054
TGCGTAA403.4615732E-739.37534
CGTTTTT145250.038.3235781
ACCGTTG1300.038.0769210
GCGATAT8250.037.6363649
TAATGCG3600.037.51
GGGCGAT159500.037.4811947
ACGGGTA4800.037.0312465
ACGGGAT23250.036.8709685
CGACCCG11600.036.85344732
GGCGATA32000.036.6328168
AGGGCGA76900.036.4856956
CGTAAGG9200.036.1956562
TAGGGAT79150.036.0739145
CTATACG1250.036.0000041
GACCGAT40050.035.8988769
TCACGAC4150.035.78313425
Produced by FastQC (version 0.11.5)