##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545379_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6904211
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2142861798401	31.0	31.0	33.0	30.0	34.0
2	31.572755235898786	31.0	31.0	34.0	30.0	34.0
3	31.74885501036976	31.0	31.0	34.0	30.0	34.0
4	35.52442516603273	37.0	35.0	37.0	33.0	37.0
5	35.42957363267142	37.0	35.0	37.0	33.0	37.0
6	35.483247687534465	37.0	35.0	37.0	33.0	37.0
7	35.88412810674529	37.0	35.0	37.0	35.0	37.0
8	35.93791933647451	37.0	35.0	37.0	35.0	37.0
9	37.825406262931416	39.0	37.0	39.0	35.0	39.0
10	37.14775794076977	39.0	37.0	39.0	33.0	39.0
11	36.77285239399549	39.0	35.0	39.0	32.0	39.0
12	36.019335011632755	37.0	35.0	39.0	32.0	39.0
13	35.69622770798865	37.0	35.0	39.0	31.0	39.0
14	36.661154909663104	38.0	35.0	41.0	31.0	41.0
15	36.91073867817771	38.0	35.0	41.0	32.0	41.0
16	37.005324142034475	38.0	35.0	41.0	32.0	41.0
17	36.930384224931714	38.0	35.0	41.0	32.0	41.0
18	36.89739812413033	38.0	35.0	41.0	32.0	41.0
19	36.86347361052552	38.0	35.0	41.0	32.0	41.0
20	36.75778579188846	38.0	35.0	41.0	31.0	41.0
21	36.62617567742353	38.0	35.0	40.0	31.0	41.0
22	36.51575639852258	38.0	35.0	40.0	31.0	41.0
23	36.43517181036327	38.0	35.0	40.0	31.0	41.0
24	36.3542711831953	38.0	35.0	40.0	31.0	41.0
25	36.229269933957696	38.0	35.0	40.0	30.0	41.0
26	36.06183907183601	38.0	35.0	40.0	30.0	41.0
27	35.939100354841415	38.0	34.0	40.0	30.0	41.0
28	35.88611906559635	37.0	35.0	40.0	30.0	41.0
29	36.02182305842043	37.0	35.0	40.0	30.0	41.0
30	36.0170203372985	37.0	35.0	40.0	30.0	41.0
31	35.916735308350226	37.0	35.0	40.0	30.0	41.0
32	35.7880970034085	37.0	34.0	40.0	30.0	41.0
33	35.63380855538743	37.0	34.0	40.0	29.0	41.0
34	35.49902878113082	37.0	34.0	40.0	29.0	41.0
35	35.38736040946605	37.0	34.0	40.0	29.0	41.0
36	35.25024032434698	37.0	34.0	40.0	27.0	41.0
37	35.14849777910901	37.0	34.0	40.0	27.0	41.0
38	35.09834244637077	37.0	34.0	40.0	27.0	41.0
39	35.05763728831578	37.0	34.0	40.0	27.0	41.0
40	34.91811185955933	36.0	34.0	40.0	26.0	41.0
41	34.87980422382804	36.0	34.0	40.0	26.0	41.0
42	34.833210485600745	36.0	34.0	40.0	26.0	41.0
43	34.7189361680864	36.0	33.0	40.0	26.0	41.0
44	34.609478186573384	36.0	33.0	40.0	26.0	41.0
45	34.572438327855274	36.0	33.0	40.0	26.0	41.0
46	34.578646133497365	36.0	33.0	40.0	26.0	41.0
47	34.54526824281587	35.0	33.0	40.0	26.0	41.0
48	34.48002255435125	35.0	33.0	40.0	26.0	41.0
49	34.45954823802459	36.0	33.0	40.0	26.0	41.0
50	34.32988345808087	35.0	33.0	40.0	26.0	41.0
51	34.095739686982334	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	8.0
12	13.0
13	29.0
14	71.0
15	146.0
16	532.0
17	1246.0
18	2767.0
19	5486.0
20	9538.0
21	15708.0
22	23936.0
23	34930.0
24	50617.0
25	71144.0
26	94242.0
27	113685.0
28	130795.0
29	154880.0
30	188554.0
31	233365.0
32	291978.0
33	379449.0
34	645392.0
35	945206.0
36	519899.0
37	639810.0
38	899662.0
39	1450247.0
40	866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.03401199644681	24.29349276839888	29.20368453397499	12.468810701179324
2	29.51162703457354	26.440457859703304	30.35165060859235	13.696264497130809
3	29.26608123650914	26.025826846833038	30.692413658852548	14.015678257805272
4	26.490224589022553	27.92907111326696	30.215748041304067	15.364956256406417
5	23.63644448293947	32.50947863557472	28.772599794531196	15.081477086954614
6	22.091097737308434	41.48337876695831	25.668465230856935	10.757058264876319
7	89.13499312231332	4.042663238420726	5.161299386707619	1.6610442525583298
8	90.21573935095553	2.9976343422876273	4.878471993396493	1.9081543133603533
9	85.43693117142567	4.903282938484933	6.600073491380839	3.059712398708556
10	49.11696644265362	25.722070197449064	12.604307718868963	12.556655641028353
11	41.16368981191334	22.752259454411227	20.361081085152236	15.7229696485232
12	36.440239152598316	22.204275043158443	25.180241449747122	16.17524435449612
13	22.36999998986126	35.933809091292254	25.493861644726675	16.20232927411981
14	18.454606905843406	37.324004147613685	27.106399268504394	17.11498967803852
15	17.805293030586697	23.97890215116543	42.0518868846853	16.163917933562573
16	19.179888911274585	19.863587598930565	40.75183971057663	20.204683779218218
17	19.310142752010332	20.71955796252461	27.549520140679363	32.4207791447857
18	23.41847605758283	23.40764208973335	31.508480838722917	21.6654010139609
19	29.113536651762235	25.22157854098028	24.154331320407213	21.510553486850274
20	31.261776327519534	24.084431950298157	23.614037867614417	21.039753854567884
21	24.63170664975332	27.68597309670866	26.15841259776099	21.523907655777034
22	24.677084753058676	24.242161776342	24.34549002051067	26.73526345008865
23	22.103771162266042	30.254144897947064	23.706517660019372	23.935566279767524
24	22.460625841243846	24.754052273315516	33.60999540715079	19.175326478289843
25	20.383183538278303	26.134427235784074	31.68924008840402	21.7931491375336
26	20.383560120048475	31.87180982736478	25.509851886044615	22.234778166542128
27	21.120183030327432	29.918480185498385	26.893934730557916	22.067402053616263
28	18.432996905801403	27.713564953330653	34.054883316862714	19.798554824005237
29	19.17206759758646	24.203620080556636	33.74947550125568	22.874836820601224
30	21.410006154215157	28.47605613443737	29.024359191803377	21.089578519544087
31	28.088017588106734	25.933767087940968	24.22113113286949	21.757084191082804
32	28.962585297581434	26.77061868474182	24.66572936429666	19.60106665338009
33	27.009371527028943	26.482446727077143	24.976134130315543	21.532047615578374
34	21.262458519880116	26.11691618347122	28.21363657628656	24.40698872036211
35	21.227103285226942	25.347921724871963	30.12392002503979	23.3010549648613
36	29.061061430480617	25.218145853306044	25.51952714075511	20.201265575458223
37	22.481091612061103	29.577963361780224	27.397120395074833	20.54382463108384
38	22.486696886870924	30.316787827023244	24.94460264902101	22.251912637084818
39	22.126452971961605	29.466900707408854	26.20729580831177	22.19935051231777
40	24.223115429119996	25.425483085612534	26.024436391066263	24.326965094201206
41	19.55912123774896	24.255733203982324	26.647389542411148	29.537756015857568
42	22.63708336839648	26.255063757466278	24.63709466584958	26.470758208287666
43	22.475718079879076	26.18386083507587	26.17564845570334	25.164772629341712
44	21.90271994873853	28.5516187150132	26.985299261566603	22.56036207468167
45	19.939917826960965	31.744177575105976	23.587604724131403	24.728299873801653
46	22.42041849532119	29.729479588616282	26.139887671451522	21.710214244611006
47	23.04195222307082	25.675562348833196	27.0429742080594	24.239511220036583
48	23.370273591001204	23.16366055440658	29.88967168008046	23.57639417451176
49	21.98313464058384	23.547730508236207	30.342250548252363	24.12688430292759
50	20.374768384106453	29.99139220976879	26.620652236729146	23.013187169395604
51	20.383444248734577	31.018591407475814	24.427367587693947	24.170596756095662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1863.0
1	4675.0
2	7487.0
3	18684.0
4	29881.0
5	22716.5
6	15552.0
7	16005.0
8	16458.0
9	17252.0
10	18046.0
11	18788.0
12	19530.0
13	19803.5
14	20077.0
15	19302.5
16	18528.0
17	18465.0
18	18402.0
19	17661.5
20	16921.0
21	19073.0
22	21225.0
23	21206.5
24	21188.0
25	26870.0
26	37095.5
27	41639.0
28	49858.0
29	58077.0
30	72954.0
31	87831.0
32	101112.5
33	114394.0
34	137501.0
35	160608.0
36	174939.5
37	189271.0
38	225477.0
39	261683.0
40	342650.0
41	423617.0
42	511570.0
43	599523.0
44	633452.5
45	667382.0
46	650086.0
47	632790.0
48	603104.0
49	573418.0
50	540502.5
51	507587.0
52	475296.0
53	443005.0
54	410339.0
55	377673.0
56	351328.5
57	324984.0
58	302746.5
59	280509.0
60	259116.0
61	237723.0
62	218422.0
63	199121.0
64	174691.0
65	150261.0
66	128729.5
67	107198.0
68	91799.0
69	76400.0
70	63129.0
71	49858.0
72	41419.0
73	32980.0
74	27467.5
75	17768.0
76	13581.0
77	10317.5
78	7054.0
79	5164.5
80	3275.0
81	2503.5
82	1732.0
83	1285.5
84	839.0
85	560.5
86	282.0
87	192.0
88	102.0
89	100.5
90	99.0
91	62.5
92	26.0
93	17.5
94	9.0
95	11.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6904211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.34059025634209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52324342683143	11.739117224973441
2	10.308545077718811	3.162783323526328
3	3.7241794654543288	1.713933336618539
4	1.7598830626593307	1.0799057985333282
5	1.0434757665215297	0.8003767088314637
6	0.6720186523086801	0.6185497673812008
7	0.4743654242530912	0.5093931923669787
8	0.33864250578871574	0.41559807397485143
9	0.2676564951165548	0.3695407758928526
>10	2.254416573815844	7.663902348182415
>50	0.7572406694632322	8.56815522204039
>100	1.843902364816528	58.94292004615295
>500	0.027089018622368552	2.7003159287868885
>1k	0.005055345024480194	1.1758342901190213
>5k	9.538386838641875E-5	0.08276112826244038
>10k+	1.907677367728375E-4	0.4569128343569596
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	15989	0.23158330473967265	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15237	0.22069140123324735	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4483914237267661E-5	0.0	0.0	0.05288077088026423	0.0
2	1.4483914237267661E-5	0.0	0.0	0.18314909553024958	0.0
3	1.4483914237267661E-5	0.0	0.0	0.2847247860762077	0.0
4	1.4483914237267661E-5	0.0	0.0	0.49398837897625086	0.0
5	1.4483914237267661E-5	0.0	0.0	0.8624736410865774	0.0
6	1.4483914237267661E-5	0.0	0.0	1.3190355856737286	0.0
7	1.4483914237267661E-5	0.0	0.0	1.5590050767567794	0.0
8	1.4483914237267661E-5	0.0	0.0	2.150716425091875	0.0
9	1.4483914237267661E-5	0.0	0.0	2.4092832620555775	0.0
10	1.4483914237267661E-5	0.0	0.0	2.7913689196346985	0.0
11	1.4483914237267661E-5	0.0	0.0	3.215211701959862	0.0
12	1.4483914237267661E-5	0.0	0.0	3.5651430699322484	0.0
13	1.4483914237267661E-5	0.0	0.0	3.711807185498821	0.0
14	1.4483914237267661E-5	0.0	0.0	3.77250927006721	0.0
15	1.4483914237267661E-5	0.0	0.0	3.86368551019081	0.0
16	1.4483914237267661E-5	0.0	0.0	4.06050742076104	0.0
17	1.4483914237267661E-5	0.0	0.0	4.294263312636303	0.0
18	1.4483914237267661E-5	0.0	0.0	4.579393648311154	1.4483914237267661E-5
19	2.8967828474535322E-5	0.0	0.0	4.739991289373978	1.4483914237267661E-5
20	2.8967828474535322E-5	0.0	0.0	4.90457200685205	1.4483914237267661E-5
21	2.8967828474535322E-5	0.0	0.0	5.1294058075571565	1.4483914237267661E-5
22	2.8967828474535322E-5	0.0	0.0	5.368665586842581	1.4483914237267661E-5
23	2.8967828474535322E-5	0.0	0.0	5.6425419211550745	1.4483914237267661E-5
24	2.8967828474535322E-5	0.0	0.0	5.854832652130707	1.4483914237267661E-5
25	2.8967828474535322E-5	0.0	0.0	6.030884629684695	1.4483914237267661E-5
26	2.8967828474535322E-5	0.0	0.0	6.197159965128528	1.4483914237267661E-5
27	2.8967828474535322E-5	0.0	0.0	6.359915709412705	1.4483914237267661E-5
28	2.8967828474535322E-5	0.0	0.0	6.534736554256526	1.4483914237267661E-5
29	2.8967828474535322E-5	0.0	0.0	6.727372613612185	1.4483914237267661E-5
30	2.8967828474535322E-5	0.0	0.0	6.964257030962698	1.4483914237267661E-5
31	2.8967828474535322E-5	0.0	0.0	7.181631615835611	1.4483914237267661E-5
32	2.8967828474535322E-5	0.0	0.0	7.386926616234643	1.4483914237267661E-5
33	2.8967828474535322E-5	0.0	0.0	7.59314858714486	1.4483914237267661E-5
34	2.8967828474535322E-5	0.0	0.0	7.816084995084884	1.4483914237267661E-5
35	2.8967828474535322E-5	0.0	0.0	8.091569043877715	1.4483914237267661E-5
36	2.8967828474535322E-5	0.0	0.0	8.321689473279424	1.4483914237267661E-5
37	2.8967828474535322E-5	0.0	0.0	8.550520834314016	1.4483914237267661E-5
38	2.8967828474535322E-5	0.0	0.0	8.77710718864183	1.4483914237267661E-5
39	2.8967828474535322E-5	0.0	0.0	9.012992215909971	1.4483914237267661E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	120	0.0	41.249996	9
TCGTTAG	270	0.0	40.833336	1
ATTACGC	45	1.9306754E-8	40.0	16
TACGGGA	1930	0.0	39.98705	4
TGCGTAA	40	3.4615732E-7	39.375	34
CGTTTTT	14525	0.0	38.323578	1
ACCGTTG	130	0.0	38.07692	10
GCGATAT	825	0.0	37.636364	9
TAATGCG	360	0.0	37.5	1
GGGCGAT	15950	0.0	37.481194	7
ACGGGTA	480	0.0	37.031246	5
ACGGGAT	2325	0.0	36.870968	5
CGACCCG	1160	0.0	36.853447	32
GGCGATA	3200	0.0	36.632816	8
AGGGCGA	7690	0.0	36.485695	6
CGTAAGG	920	0.0	36.195656	2
TAGGGAT	7915	0.0	36.073914	5
CTATACG	125	0.0	36.000004	1
GACCGAT	4005	0.0	35.898876	9
TCACGAC	415	0.0	35.783134	25
>>END_MODULE