FastQCFastQC Report
Thu 26 May 2016
SRR1545378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545378_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2284551
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52910.23159911947686876No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51440.22516459470591815No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.03475E-445.00000421
ATCGTAC207.03475E-445.00000411
GCGATCG207.03475E-445.0000049
AATCGTA600.045.00000412
CGAACTA253.8920196E-545.025
CGTACTC253.8920196E-545.031
CAATACG700.045.01
TACGTAG1100.045.01
TATTACG750.042.0000041
ACGTATG750.039.0000041
CGTTAGT356.2510007E-638.57142623
TATCGTG700.038.5714261
CACGACC3100.038.4677427
TATGGGC7650.037.9411744
TATGGGA13050.037.9310344
TGTTGCG1200.037.5000041
CACTCGA301.1402749E-437.50000428
CGAATCG301.1402749E-437.50000436
ATTGCGT601.5643309E-1037.50000414
TGTACGC301.1402749E-437.50000421