##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545378_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2284551
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206305746730976	31.0	31.0	33.0	30.0	34.0
2	31.569914613418568	31.0	31.0	34.0	30.0	34.0
3	31.74957311086511	31.0	31.0	34.0	30.0	34.0
4	35.53737867966178	37.0	35.0	37.0	33.0	37.0
5	35.440595110373984	37.0	35.0	37.0	33.0	37.0
6	35.49240222695838	37.0	35.0	37.0	33.0	37.0
7	35.84390762123498	37.0	35.0	37.0	35.0	37.0
8	35.886225783534705	37.0	35.0	37.0	35.0	37.0
9	37.76741469111436	39.0	37.0	39.0	35.0	39.0
10	37.153792145590096	39.0	37.0	39.0	33.0	39.0
11	36.74515867669402	39.0	35.0	39.0	32.0	39.0
12	35.87994183539785	37.0	35.0	39.0	31.0	39.0
13	35.48929658388016	37.0	35.0	39.0	30.0	39.0
14	36.42528488092409	38.0	35.0	41.0	31.0	41.0
15	36.71525476997449	38.0	35.0	41.0	31.0	41.0
16	36.86751488585722	38.0	35.0	41.0	32.0	41.0
17	36.79148856821318	38.0	35.0	41.0	32.0	41.0
18	36.72306505742266	38.0	35.0	40.0	31.0	41.0
19	36.67771303857957	38.0	35.0	40.0	31.0	41.0
20	36.54470615889074	38.0	35.0	40.0	31.0	41.0
21	36.40875909533208	38.0	35.0	40.0	31.0	41.0
22	36.27033364542967	38.0	35.0	40.0	31.0	41.0
23	36.20640423435502	38.0	35.0	40.0	31.0	41.0
24	36.08373461568597	37.0	35.0	40.0	30.0	41.0
25	35.95289271283504	37.0	34.0	40.0	30.0	41.0
26	35.7795553699611	37.0	34.0	40.0	30.0	41.0
27	35.649657635132684	37.0	34.0	40.0	30.0	41.0
28	35.58067340146926	37.0	34.0	40.0	29.0	41.0
29	35.7294650020945	37.0	34.0	40.0	30.0	41.0
30	35.72141002761593	37.0	34.0	40.0	30.0	41.0
31	35.59600245299842	37.0	34.0	40.0	30.0	41.0
32	35.45250729793294	37.0	34.0	40.0	29.0	41.0
33	35.294028892329386	36.0	34.0	40.0	29.0	41.0
34	35.132062273943546	36.0	34.0	40.0	27.0	41.0
35	34.99885841900662	36.0	34.0	40.0	27.0	41.0
36	34.826175471679115	36.0	34.0	40.0	26.0	41.0
37	34.7029066105331	36.0	33.0	40.0	26.0	41.0
38	34.66030743021276	36.0	33.0	40.0	26.0	41.0
39	34.61905380969827	36.0	33.0	40.0	26.0	41.0
40	34.45950911141839	36.0	33.0	40.0	25.0	41.0
41	34.39505487073828	35.0	33.0	40.0	24.0	41.0
42	34.314003057931295	35.0	33.0	40.0	24.0	41.0
43	34.186810449843314	35.0	33.0	40.0	24.0	41.0
44	34.051069991433764	35.0	33.0	40.0	24.0	41.0
45	34.00729640091204	35.0	33.0	40.0	23.0	41.0
46	34.009973513394975	35.0	33.0	40.0	24.0	41.0
47	33.958953860080165	35.0	33.0	39.0	23.0	41.0
48	33.88893441205734	35.0	33.0	39.0	23.0	41.0
49	33.84111101043487	35.0	33.0	39.0	24.0	41.0
50	33.73098565100976	35.0	33.0	39.0	24.0	41.0
51	33.48954258407888	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	5.0
13	12.0
14	27.0
15	81.0
16	213.0
17	580.0
18	1213.0
19	2354.0
20	4189.0
21	6606.0
22	9683.0
23	13908.0
24	19278.0
25	26618.0
26	35095.0
27	42481.0
28	48211.0
29	55592.0
30	66484.0
31	80711.0
32	100545.0
33	130681.0
34	229954.0
35	326671.0
36	168672.0
37	207158.0
38	282377.0
39	424893.0
40	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00887088972844	23.94479265291079	28.299390120859634	13.746946336501134
2	30.32009353260225	26.081974094690818	29.723652481384743	13.874279891322189
3	29.5405092729381	25.585421380393786	30.71329990006789	14.16076944660023
4	27.258704226782417	28.006203407146522	29.20070508384361	15.53438728222745
5	23.89953211812737	32.66541215319772	28.689138478414357	14.745917250260554
6	22.70433008499263	41.36300743559676	25.34861335991186	10.584049119498754
7	88.88525578986855	4.025254853141821	5.281694302293974	1.8077950546956494
8	90.06566279325784	3.042961177054047	5.038057806544918	1.853318223143191
9	85.15935954154668	4.8843295684797585	6.868658217741692	3.087652672231874
10	54.14350566041205	20.703499287168466	12.386635273189349	12.766359779230141
11	45.544222912948754	21.16761674394662	17.98265829915813	15.305502043946491
12	40.13589541227138	20.93273470366825	23.225701680549044	15.705668203511324
13	23.46434813667981	35.99302444988096	23.83903883082496	16.703588582614266
14	18.187381240340006	37.486797186843276	26.598049244687466	17.727772328129245
15	17.149059049239874	22.72039451078133	43.304658114439114	16.82588832553968
16	19.499542798563045	17.628190397150252	41.942552387755846	20.92971441653086
17	19.67257461094106	18.83604261844012	27.702336257759185	33.78904651285964
18	24.127585683138612	22.411143371279522	30.54004922630311	22.921221719278755
19	30.79103946464754	23.337145898690814	23.56393006765881	22.307884569002837
20	32.12543733976611	23.015419660143284	22.725209461290206	22.133933538800406
21	26.3150614715977	25.61531784582616	25.57408436055925	22.49553632201689
22	25.11635765627469	24.930806972573606	23.965015445048063	25.98781992610364
23	24.076372118635128	28.73041573595862	23.40586837413566	23.787343771270592
24	24.081756108749598	23.880053454705106	32.59642704408875	19.441763392456547
25	21.58520426989811	24.238154455733316	31.00876277220338	23.167878502165195
26	21.09044621897257	30.15415282915549	25.184336003004532	23.571064948867416
27	21.976134478941376	28.732516805271587	27.150280295778035	22.14106842000901
28	18.72980730130341	27.565635435584497	34.03005667196749	19.674500591144607
29	19.651345056424656	24.503064278276128	33.5617370765634	22.283853588735816
30	22.212373459817705	26.626457452689827	29.494198203498197	21.66697088399427
31	28.76976701329933	25.158160181147192	23.517049958613313	22.555022846940165
32	30.546396206519354	25.666925360825825	23.956173445022678	19.830504987632143
33	28.15638609074606	25.96864766862285	23.92846559345797	21.94650064717312
34	21.61615126998697	25.67287839054589	28.666727072409415	24.044243267057727
35	23.227058621147002	24.037108385849123	29.17606129169364	23.559771701310236
36	29.563971213599523	24.660075437142794	25.19409722085434	20.58185612840335
37	23.99412400948808	28.750157033045003	27.221147612813194	20.03457134465372
38	23.300596047100722	29.551058391780266	23.83321711793696	23.31512844318205
39	23.087906551440522	29.168401143156796	25.22162998330963	22.52206232209305
40	24.89745249723031	25.347256419313908	24.769637447358363	24.98565363609742
41	20.066787740785827	23.57833114690808	26.177135025657122	30.177746086648977
42	23.516218285343594	26.645060670565023	22.827942996238647	27.010778047852728
43	24.033694148215556	26.391838046075573	24.858101219889598	24.716366585819273
44	22.399105995007336	28.9357952613008	25.404466785814805	23.260631957877063
45	20.55926963328899	30.909093296669678	22.889749451861658	25.641887618179677
46	23.647710206513228	29.433573599363726	25.479492469198544	21.439223724924503
47	24.275448436038417	25.18459863666865	27.21830241478522	23.32165051250771
48	25.087555497776147	22.362249737475768	28.562549052308313	23.987645712439775
49	21.79496102297563	23.210293838920645	29.977619234589202	25.01712590351452
50	20.775592227969522	28.653639161480747	26.782899571950903	23.78786903859883
51	20.448219365643403	30.468481552830294	24.365794416495845	24.71750466503046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	600.0
1	1859.5
2	3119.0
3	7142.0
4	11165.0
5	8478.5
6	5792.0
7	5936.0
8	6080.0
9	6376.0
10	6672.0
11	6862.5
12	7053.0
13	7040.5
14	7028.0
15	6824.0
16	6620.0
17	6279.0
18	5938.0
19	5715.0
20	5492.0
21	5959.5
22	6427.0
23	6891.0
24	7355.0
25	8419.0
26	11671.5
27	13860.0
28	17911.5
29	21963.0
30	25183.0
31	28403.0
32	31845.0
33	35287.0
34	39545.0
35	43803.0
36	46778.5
37	49754.0
38	60868.0
39	71982.0
40	100344.5
41	128707.0
42	154102.5
43	179498.0
44	181983.5
45	184469.0
46	181321.0
47	178173.0
48	172586.0
49	166999.0
50	164925.0
51	162851.0
52	154033.5
53	145216.0
54	137354.0
55	129492.0
56	124381.0
57	119270.0
58	119253.0
59	119236.0
60	115544.5
61	111853.0
62	101374.0
63	90895.0
64	78434.5
65	65974.0
66	56436.5
67	46899.0
68	40136.0
69	33373.0
70	29542.0
71	25711.0
72	21779.5
73	17848.0
74	14297.0
75	8634.5
76	6523.0
77	4777.5
78	3032.0
79	2509.0
80	1986.0
81	1551.5
82	1117.0
83	822.5
84	528.0
85	335.0
86	142.0
87	113.5
88	85.0
89	59.0
90	33.0
91	22.0
92	11.0
93	8.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2284551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.6841044467055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71390480344452	14.273531228455576
2	7.0364440774233	2.488664239971119
3	2.121426775214592	1.125465980067974
4	0.9974440389928186	0.7055561826117119
5	0.5954924770447612	0.5265375580643467
6	0.44024146069942366	0.4671165583667281
7	0.31864466946563347	0.39444619313513485
8	0.27115752855784425	0.38361424452219584
9	0.22014833139080517	0.35038134774746404
>10	3.587550703401891	16.893584813775593
>50	2.4349701357266698	32.19572519052157
>100	1.2555966923121091	28.89799222433302
>500	0.0054829549701707015	0.5856012628999949
>1k	9.969009036674002E-4	0.25187861951331914
>5k	4.984504518337001E-4	0.45990435601425805
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5291	0.23159911947686876	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5144	0.22516459470591815	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04145234665367505	0.0
2	0.0	0.0	0.0	0.14134068357414653	0.0
3	0.0	0.0	0.0	0.20367240652539603	0.0
4	0.0	0.0	0.0	0.31581698110482104	0.0
5	0.0	0.0	0.0	0.5109975658236564	0.0
6	0.0	0.0	0.0	0.7387884971707789	0.0
7	0.0	0.0	0.0	0.8712871807195375	0.0
8	0.0	0.0	0.0	1.2279874688724393	0.0
9	0.0	0.0	0.0	1.3810153504999452	0.0
10	0.0	0.0	0.0	1.6326621730046735	0.0
11	0.0	0.0	0.0	1.9164378470868018	0.0
12	0.0	0.0	0.0	2.1514949764745896	0.0
13	0.0	0.0	0.0	2.2488445213085635	0.0
14	0.0	0.0	0.0	2.286532452109846	0.0
15	0.0	0.0	0.0	2.3473759176310796	0.0
16	0.0	0.0	0.0	2.4873596606072703	0.0
17	0.0	0.0	0.0	2.6428387897665666	0.0
18	0.0	0.0	0.0	2.8370126121062738	0.0
19	0.0	0.0	0.0	2.9358066420929103	0.0
20	0.0	0.0	0.0	3.0460690087461386	0.0
21	0.0	0.0	0.0	3.1919620091650396	0.0
22	0.0	0.0	0.0	3.343545405639883	0.0
23	0.0	0.0	0.0	3.5117622675090203	0.0
24	0.0	0.0	0.0	3.642116109467462	0.0
25	0.0	0.0	0.0	3.750890218690675	0.0
26	0.0	0.0	0.0	3.85287962492411	0.0
27	0.0	0.0	0.0	3.9561384272007936	0.0
28	0.0	0.0	0.0	4.063511823548697	0.0
29	0.0	0.0	0.0	4.178589140710801	0.0
30	0.0	0.0	0.0	4.316778220315502	0.0
31	0.0	0.0	0.0	4.450327438520742	0.0
32	0.0	0.0	0.0	4.582213310186553	0.0
33	0.0	0.0	0.0	4.712348290758228	0.0
34	0.0	0.0	0.0	4.853470112945607	0.0
35	0.0	0.0	0.0	5.018491598567946	0.0
36	0.0	0.0	0.0	5.157512351442362	0.0
37	0.0	0.0	0.0	5.299290757790042	0.0
38	0.0	0.0	0.0	5.441725748298024	0.0
39	0.0	0.0	0.0	5.59077035268637	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.03475E-4	45.000004	21
ATCGTAC	20	7.03475E-4	45.000004	11
GCGATCG	20	7.03475E-4	45.000004	9
AATCGTA	60	0.0	45.000004	12
CGAACTA	25	3.8920196E-5	45.0	25
CGTACTC	25	3.8920196E-5	45.0	31
CAATACG	70	0.0	45.0	1
TACGTAG	110	0.0	45.0	1
TATTACG	75	0.0	42.000004	1
ACGTATG	75	0.0	39.000004	1
CGTTAGT	35	6.2510007E-6	38.571426	23
TATCGTG	70	0.0	38.571426	1
CACGACC	310	0.0	38.46774	27
TATGGGC	765	0.0	37.941174	4
TATGGGA	1305	0.0	37.931034	4
TGTTGCG	120	0.0	37.500004	1
CACTCGA	30	1.1402749E-4	37.500004	28
CGAATCG	30	1.1402749E-4	37.500004	36
ATTGCGT	60	1.5643309E-10	37.500004	14
TGTACGC	30	1.1402749E-4	37.500004	21
>>END_MODULE