FastQCFastQC Report
Thu 26 May 2016
SRR1545377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545377_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5189507
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435160.8385382272343018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT433300.043.587581
TACGGGA12400.038.286294
CGTTAGG8800.038.0965922
GTTTTTT513400.037.821392
GGGCGAT110750.037.320547
AGGGCGA59150.037.2020266
TGGGCGA33250.037.150386
TAATGCG2150.036.6279071
CGAGGGT12100.036.446284
TAGGGCG25150.036.3220675
GGCGATA23750.036.2842068
TACGGGT9800.036.275514
CGTAAGG8950.035.6983222
TCGATAG2650.035.6603781
ATAGGGC32050.035.522624
TATGGGC29850.035.2763824
TAGGGTA32700.035.229365
GCGATCG3400.035.073539
ACGGGTA8250.034.9090925
GGCGATT31350.034.7368438