FastQCFastQC Report
Thu 26 May 2016
SRR1545376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545376_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3200787
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.253406427856649No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49200.15371219640669623No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34420.10753605285200171No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC207.035175E-445.00000419
TAATGCG1050.040.7142871
CTATGCG850.039.7058831
GTACGAG3600.038.751
CGTTTTT70650.038.3121031
GGGCGAT77200.037.9760367
GCGATAA4250.037.5882389
CGTACGT301.1403773E-437.49999631
CGACTTA301.1403773E-437.49999622
AGGGCGA43950.037.4744036
TAGGGCG12900.037.151165
TATGGGC13050.036.5517234
CATACGA9250.036.48648518
ATACTAT20050.036.47131745
TGGGCGA21750.036.4137956
CGTTAGG5550.036.081082
TAGTTCG751.8189894E-1236.0000041
GTATACG1750.036.0000041
GTCGAAT250.002107669636.038
GTATCGA250.002107669636.026
Produced by FastQC (version 0.11.5)