FastQCFastQC Report
Thu 26 May 2016
SRR1545375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545375_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3701604
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC114040.30808265822059844No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80220.21671686112290778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG800.045.0000041
GACCGAA207.035318E-445.0000048
TACGATC502.1827873E-1145.011
CGGTAAT253.8924907E-545.06
ATACCGG453.8562575E-1045.02
CTAGCGA253.8924907E-545.016
TTCGATC302.1668147E-644.99999617
CTCGGTA750.042.021
TACACGG2200.041.931822
TCGGAAT556.184564E-1140.90909230
TGCGATA501.0822987E-940.51
CGTTTAG1000.040.530
TATCACG950.040.2631571
TATCGTT451.9303116E-840.032
ATCTACG403.460991E-739.37500424
ACCGTAC403.460991E-739.37500414
ATACGCC800.039.37500419
CGTATGG3900.039.230772
ATTGGGC8000.039.093754
TAGCATA11000.039.06818430
Produced by FastQC (version 0.11.5)