##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545375_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3701604
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26909199363303	31.0	31.0	33.0	30.0	34.0
2	31.632425294547986	31.0	31.0	34.0	30.0	34.0
3	31.82507313045912	31.0	31.0	34.0	30.0	34.0
4	35.59103972223933	37.0	35.0	37.0	33.0	37.0
5	35.51147961802505	37.0	35.0	37.0	33.0	37.0
6	35.55612972106147	37.0	35.0	37.0	33.0	37.0
7	35.89180014934067	37.0	35.0	37.0	35.0	37.0
8	35.93349288578681	37.0	35.0	37.0	35.0	37.0
9	37.84003853464606	39.0	38.0	39.0	35.0	39.0
10	37.24802815212	39.0	37.0	39.0	34.0	39.0
11	36.85303425217825	39.0	35.0	39.0	33.0	39.0
12	35.84323903907603	37.0	35.0	39.0	32.0	39.0
13	35.343159073742086	37.0	35.0	39.0	30.0	39.0
14	36.25082045513243	38.0	35.0	41.0	31.0	41.0
15	36.6040270650237	38.0	35.0	41.0	31.0	41.0
16	36.780055619131595	38.0	35.0	41.0	32.0	41.0
17	36.74326211015549	38.0	35.0	41.0	32.0	41.0
18	36.6988327222469	38.0	35.0	40.0	32.0	41.0
19	36.65025486248664	38.0	35.0	40.0	32.0	41.0
20	36.50934621855823	38.0	35.0	40.0	31.0	41.0
21	36.37135955115674	37.0	35.0	40.0	31.0	41.0
22	36.22845339479858	37.0	35.0	40.0	31.0	41.0
23	36.177125916224426	37.0	35.0	40.0	31.0	41.0
24	36.088597807869235	37.0	35.0	40.0	31.0	41.0
25	35.9507454065859	36.0	35.0	40.0	31.0	41.0
26	35.78002806350977	36.0	34.0	40.0	30.0	41.0
27	35.66666531590089	36.0	34.0	40.0	30.0	41.0
28	35.63985909892036	36.0	34.0	40.0	30.0	41.0
29	35.78642312900029	36.0	35.0	40.0	30.0	41.0
30	35.80064047910041	36.0	35.0	40.0	30.0	41.0
31	35.66114230479543	36.0	34.0	40.0	30.0	41.0
32	35.52693859202659	36.0	34.0	40.0	30.0	41.0
33	35.36471783583549	36.0	34.0	40.0	29.0	41.0
34	35.249052843037774	36.0	34.0	40.0	29.0	41.0
35	35.0970001113031	36.0	34.0	40.0	28.0	41.0
36	34.912239937065124	36.0	34.0	40.0	27.0	41.0
37	34.80070585616398	36.0	34.0	40.0	27.0	41.0
38	34.77798219366523	35.0	33.0	40.0	27.0	41.0
39	34.73935704629669	35.0	34.0	40.0	27.0	41.0
40	34.562547209263876	35.0	33.0	40.0	26.0	41.0
41	34.543575703938075	35.0	34.0	40.0	26.0	41.0
42	34.510490857476924	35.0	33.0	40.0	26.0	41.0
43	34.376492461106054	35.0	33.0	40.0	26.0	41.0
44	34.26439078842577	35.0	33.0	40.0	26.0	41.0
45	34.23639427664332	35.0	33.0	39.0	26.0	41.0
46	34.25749350821968	35.0	33.0	39.0	26.0	41.0
47	34.225902068400615	35.0	33.0	39.0	26.0	41.0
48	34.17421879812103	35.0	33.0	39.0	26.0	41.0
49	34.14686254931646	35.0	33.0	39.0	26.0	41.0
50	34.008198878107976	35.0	33.0	39.0	26.0	41.0
51	33.76826343390595	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	7.0
12	11.0
13	14.0
14	35.0
15	105.0
16	304.0
17	745.0
18	1546.0
19	3148.0
20	5519.0
21	8797.0
22	13041.0
23	19165.0
24	27231.0
25	38669.0
26	51174.0
27	62329.0
28	71873.0
29	84628.0
30	102660.0
31	127994.0
32	163106.0
33	216799.0
34	396648.0
35	602497.0
36	265147.0
37	319323.0
38	439360.0
39	679283.0
40	438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.597921333562425	24.416036939661833	29.15773810488642	12.828303621889322
2	29.640123578859328	26.02363732046972	30.708984537514006	13.627254563156946
3	29.98691918422392	25.821967990092944	29.94685547130379	14.244257354379345
4	26.098496759783057	28.073559462330383	30.394121035097214	15.43382274278934
5	24.229090956244914	33.468761110048504	28.027201180893474	14.274946752813106
6	22.47050197698079	41.41496497194189	25.442808036732185	10.671725014345132
7	88.42334296159179	4.59149601091851	5.219791204029389	1.7653698234603161
8	89.9525989273839	3.0591062685257526	4.9162471188165995	2.0720476852737355
9	85.69366145054954	4.548136429504615	6.853839578733976	2.9043625412118637
10	56.48732279303783	19.133840356775064	12.192471155747617	12.186365694439491
11	48.69775373054492	20.030829878074478	16.318871494627736	14.952544896752867
12	42.55798297170632	20.643510218813248	22.099932893956243	14.698573915524188
13	23.03036737587273	39.07762688823548	22.316784831656765	15.57522090423503
14	17.188548531933723	41.85061395006057	25.58077525310649	15.380062264899216
15	16.08583738292913	21.81230082958631	46.923198699806896	15.178663087677666
16	17.163748472283906	16.25865435632769	45.86838570522401	20.709211466164398
17	18.34820796606012	17.033777789304313	27.360841408211144	37.257172836424424
18	22.544929171245762	22.385754932186156	32.39274109278032	22.676574803787762
19	31.73716043099154	22.310328171246844	23.90277296004651	22.04973843771511
20	33.871640510438176	21.865845184952253	22.155071152938024	22.107443151671546
21	24.67168287045292	26.78611758578173	25.390479370564762	23.15172017320059
22	24.730711334869966	24.458126801246163	22.76172707831524	28.049434785568632
23	22.25527095821163	29.806375830585875	22.552736597431817	25.38561661377068
24	22.826401743676524	23.049034958898897	34.83722191784967	19.28734137957491
25	19.83442853422462	23.439028053784252	33.80377803784522	22.92276537414591
26	18.83421349231306	31.909491128710687	26.073939837972944	23.182355541003304
27	20.8899439270111	30.579959390577706	26.990569493657347	21.539527188753848
28	17.097723041146487	27.632048160743288	36.03994376491921	19.230285033191016
29	17.505113999228442	23.8915616041046	35.2952125619056	23.308111834761363
30	20.523778340416747	28.09220003004103	29.441858178238405	21.942163451303813
31	29.185888063661054	25.708125450480384	22.426358951416738	22.679627534441828
32	31.15814117339402	25.397395291338565	24.002783658111458	19.441679877155956
33	28.88523461720919	26.42046529018231	23.622975337178154	21.07132475543035
34	21.100150097093042	25.93697218827298	27.44388648812785	25.51899122650613
35	21.63583678859219	24.54127994242496	29.810374097283233	24.01250917169962
36	31.266040343591587	23.71912284512336	24.772612089245634	20.242224722039417
37	22.54682024333235	30.388285726944318	27.079530927673517	19.985363102049813
38	22.58628961931098	31.717925526339393	22.136619692436035	23.559165161913594
39	22.06994589372607	30.349329641960622	25.27809565799043	22.30262880632288
40	24.724849011401545	24.759995936896544	24.25859168079568	26.256563370906232
41	18.61128310861994	22.778233436099594	25.81072961883551	32.79975383644496
42	22.881161788240988	26.035172860197903	23.025207450607898	28.05845790095321
43	22.574943186791455	26.105547757134474	25.361329845115794	25.958179210958278
44	21.662230751857845	28.832878935726242	26.22203779766825	23.28285251474766
45	19.076243704080717	33.31342304579312	22.232740185065715	25.377593065060445
46	22.70167203190833	30.719682602460985	25.456018526022774	21.12262683960791
47	23.145830834416646	25.29932969599125	26.903823315514032	24.65101615407807
48	24.35957493021944	22.028315292505628	28.913006361566502	24.699103415708436
49	21.73582047134161	21.892914531105976	31.19520618629113	25.17605881126128
50	20.1293817491012	30.38091054580663	26.081693233527954	23.408014471564222
51	19.81803023770236	32.31790866878251	22.48530636988722	25.378754723627917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1306.0
1	2792.0
2	4278.0
3	9897.0
4	15516.0
5	11881.0
6	8246.0
7	8554.0
8	8862.0
9	9416.5
10	9971.0
11	10054.0
12	10137.0
13	10004.0
14	9871.0
15	10122.5
16	10374.0
17	9635.0
18	8896.0
19	8859.0
20	8822.0
21	8640.5
22	8459.0
23	9661.0
24	10863.0
25	13107.0
26	16503.5
27	17656.0
28	23739.0
29	29822.0
30	32527.5
31	35233.0
32	41821.5
33	48410.0
34	54844.0
35	61278.0
36	66198.0
37	71118.0
38	94371.0
39	117624.0
40	170954.5
41	224285.0
42	286678.0
43	349071.0
44	361690.5
45	374310.0
46	351799.0
47	329288.0
48	302897.5
49	276507.0
50	266352.5
51	256198.0
52	246613.0
53	237028.0
54	219890.0
55	202752.0
56	192001.5
57	181251.0
58	175091.0
59	168931.0
60	165165.0
61	161399.0
62	146510.5
63	131622.0
64	114672.5
65	97723.0
66	82938.0
67	68153.0
68	57572.5
69	46992.0
70	39122.0
71	31252.0
72	25739.0
73	20226.0
74	17782.0
75	11682.5
76	8027.0
77	6579.0
78	5131.0
79	3629.0
80	2127.0
81	1640.5
82	1154.0
83	742.5
84	331.0
85	285.5
86	240.0
87	160.0
88	80.0
89	66.5
90	53.0
91	39.5
92	26.0
93	19.0
94	12.0
95	8.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3701604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.818698335618077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82190813711597	11.680380789217216
2	9.182877455035989	2.7215658171825314
3	3.0511745127746246	1.3564330402240088
4	1.403465504164651	0.8319012772264841
5	0.8091012341994772	0.5994913556289162
6	0.5310091796479905	0.472131890798856
7	0.3431781690173493	0.35598176334264975
8	0.2754929082299856	0.3265957040529818
9	0.21398046603467025	0.2853820778294474
>10	2.0603420140087967	7.168234695002986
>50	1.0274740357059056	11.272178377087467
>100	2.254759301371814	59.66645484391708
>500	0.022105473891363376	2.1119264247722445
>1k	0.002763184236420422	0.6210536681487131
>5k	1.8421228242802815E-4	0.2189834524147939
>10k+	1.8421228242802815E-4	0.31130482315361624
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11404	0.30808265822059844	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8022	0.21671686112290778	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05343629410385336	0.0
2	0.0	0.0	0.0	0.20985497098014808	0.0
3	0.0	0.0	0.0	0.31451230331499536	0.0
4	0.0	0.0	0.0	0.5223681409464653	0.0
5	0.0	0.0	0.0	0.8554399660255392	0.0
6	0.0	0.0	0.0	1.2219027210906408	0.0
7	0.0	0.0	0.0	1.4221132244291934	0.0
8	0.0	0.0	0.0	1.912008956117402	0.0
9	0.0	0.0	0.0	2.094416366526511	0.0
10	0.0	0.0	0.0	2.3907473624947455	0.0
11	0.0	0.0	0.0	2.7404876372513107	0.0
12	0.0	0.0	0.0	3.0237432205065695	0.0
13	0.0	0.0	0.0	3.1399631078851225	0.0
14	0.0	0.0	0.0	3.187104833472192	0.0
15	0.0	0.0	0.0	3.2669080755261772	0.0
16	0.0	0.0	0.0	3.444587805718818	0.0
17	2.7015315522676117E-5	0.0	0.0	3.641529455879127	0.0
18	2.7015315522676117E-5	0.0	0.0	3.9123309786784324	0.0
19	2.7015315522676117E-5	0.0	0.0	4.0351155877289955	0.0
20	2.7015315522676117E-5	0.0	0.0	4.167085404057268	0.0
21	2.7015315522676117E-5	0.0	0.0	4.350411335194148	0.0
22	2.7015315522676117E-5	0.0	0.0	4.528982570799037	0.0
23	2.7015315522676117E-5	0.0	0.0	4.740755629181296	0.0
24	2.7015315522676117E-5	0.0	0.0	4.901226603385991	0.0
25	2.7015315522676117E-5	0.0	0.0	5.025659146683438	0.0
26	2.7015315522676117E-5	0.0	0.0	5.151712608912245	0.0
27	2.7015315522676117E-5	0.0	0.0	5.269256246751408	0.0
28	2.7015315522676117E-5	0.0	0.0	5.3958500152906685	0.0
29	2.7015315522676117E-5	0.0	0.0	5.5264150352117625	0.0
30	2.7015315522676117E-5	0.0	0.0	5.69931305455689	0.0
31	2.7015315522676117E-5	0.0	0.0	5.866132627909415	0.0
32	2.7015315522676117E-5	0.0	0.0	6.013933419133975	0.0
33	2.7015315522676117E-5	0.0	0.0	6.16611069147321	0.0
34	2.7015315522676117E-5	0.0	0.0	6.325852252158794	0.0
35	2.7015315522676117E-5	0.0	0.0	6.532249262752039	0.0
36	2.7015315522676117E-5	0.0	0.0	6.700203479356516	0.0
37	2.7015315522676117E-5	0.0	0.0	6.875262723943458	0.0
38	2.7015315522676117E-5	0.0	0.0	7.037732831496832	0.0
39	2.7015315522676117E-5	0.0	0.0	7.20730796703267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	80	0.0	45.000004	1
GACCGAA	20	7.035318E-4	45.000004	8
TACGATC	50	2.1827873E-11	45.0	11
CGGTAAT	25	3.8924907E-5	45.0	6
ATACCGG	45	3.8562575E-10	45.0	2
CTAGCGA	25	3.8924907E-5	45.0	16
TTCGATC	30	2.1668147E-6	44.999996	17
CTCGGTA	75	0.0	42.0	21
TACACGG	220	0.0	41.93182	2
TCGGAAT	55	6.184564E-11	40.909092	30
TGCGATA	50	1.0822987E-9	40.5	1
CGTTTAG	100	0.0	40.5	30
TATCACG	95	0.0	40.263157	1
TATCGTT	45	1.9303116E-8	40.0	32
ATCTACG	40	3.460991E-7	39.375004	24
ACCGTAC	40	3.460991E-7	39.375004	14
ATACGCC	80	0.0	39.375004	19
CGTATGG	390	0.0	39.23077	2
ATTGGGC	800	0.0	39.09375	4
TAGCATA	1100	0.0	39.068184	30
>>END_MODULE