FastQCFastQC Report
Thu 26 May 2016
SRR1545374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545374_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences222502
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.4853888953807157No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA4320.19415555815228627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA302.157587E-645.00000438
AGGCCGA302.157587E-645.00000429
AGATTTG302.157587E-645.0000041
TATAAGG302.157587E-645.0000042
AGCCGTT351.2060264E-745.045
TTGTCAG207.0210156E-445.01
TAAACGG207.0210156E-445.02
CGACCCG207.0210156E-445.032
TAACGGG406.7720975E-945.03
CGACAAT207.0210156E-445.020
ACGGGCT253.8806367E-545.05
GGTATGC406.7720975E-945.08
GTTCCAC207.0210156E-445.036
CATACGA351.2060264E-745.018
GAGTTAC207.0210156E-445.044
TAGATAG253.8806367E-545.01
TATACGG207.0210156E-445.02
TAGTAAG207.0210156E-445.01
TAAGTAG603.6379788E-1241.2500041
CGTTTTT7550.040.8278161