FastQCFastQC Report
Thu 26 May 2016
SRR1545373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545373_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5396459
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133860.24805154639366295No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC107810.19977915147692216No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT71750.13295755605666607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG3150.040.01
GCTACGA9450.039.5238110
CGTTTTT123400.038.8006481
ACTACGG2500.038.72
TACGGGA14750.038.593224
TAATACG1400.038.5714261
CGCGATA356.2525814E-638.57142632
TTGCGAG4800.038.4374961
CACGACC7900.038.16455527
CGAATAT9850.037.91878514
TACGAAT9850.037.69035712
CGTAAGG9850.037.6903572
ACTATCG301.1404812E-437.49999624
GGTATGC14150.037.208488
ACGGGAC12650.037.1739165
TAGGGTA27550.037.159715
GCGATCG552.75395E-936.818189
AGGGCGA62500.036.7926
CGAGGGA27400.036.7883224
GGGCGAT128300.036.704997
Produced by FastQC (version 0.11.5)