FastQCFastQC Report
Thu 26 May 2016
SRR1545372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545372_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2297784
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56400.24545388078252786No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42390.18448209231154886No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCG1250.045.0000041
AATCGGT253.8920265E-545.00000418
CGTCTAC453.8562575E-1045.041
TCTACGA207.0347614E-445.039
TCGAACG207.0347614E-445.01
TAATGCG900.042.51
CGTTTTT48150.041.6822431
CGTTGAT2550.041.4705925
CGAGACA5250.040.28571322
GCGTAAG1900.040.2631571
TACGGGA7000.039.2142874
AATGCGG4250.039.1764682
TATTCCG750.039.01
GTTACGG2450.038.571432
TGCGTAG1050.038.571431
TATCTCG356.251017E-638.5714261
TGTTACG1000.038.2500041
TATCACG659.094947E-1238.0769271
CGTTAGG3250.038.0769232
ACTACGG1600.037.968752
Produced by FastQC (version 0.11.5)