##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545372_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2297784
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31013620079172	31.0	31.0	33.0	30.0	34.0
2	31.685344662509618	31.0	31.0	34.0	30.0	34.0
3	31.862838282449527	31.0	31.0	34.0	30.0	34.0
4	35.6290978612437	37.0	35.0	37.0	33.0	37.0
5	35.56624904690781	37.0	35.0	37.0	33.0	37.0
6	35.63385461818866	37.0	35.0	37.0	33.0	37.0
7	35.9663114548626	37.0	35.0	37.0	35.0	37.0
8	36.019793853556294	37.0	35.0	37.0	35.0	37.0
9	37.93541473001814	39.0	38.0	39.0	35.0	39.0
10	37.28300745413842	39.0	37.0	39.0	34.0	39.0
11	36.91546681498348	39.0	37.0	39.0	33.0	39.0
12	36.15293561100608	37.0	35.0	39.0	32.0	39.0
13	35.8275695191541	37.0	35.0	39.0	31.0	39.0
14	36.7941642904642	38.0	35.0	41.0	31.0	41.0
15	37.06102662391243	38.0	35.0	41.0	32.0	41.0
16	37.16528490058248	38.0	35.0	41.0	32.0	41.0
17	37.10762108187715	38.0	35.0	41.0	32.0	41.0
18	37.07595970726578	38.0	35.0	41.0	32.0	41.0
19	37.055871222012165	38.0	35.0	41.0	32.0	41.0
20	36.93439374632254	38.0	35.0	41.0	32.0	41.0
21	36.80458041312848	38.0	35.0	41.0	32.0	41.0
22	36.680839452272274	38.0	35.0	41.0	31.0	41.0
23	36.61598522750615	38.0	35.0	40.0	31.0	41.0
24	36.53552988444519	38.0	35.0	40.0	31.0	41.0
25	36.4414174700494	38.0	35.0	40.0	31.0	41.0
26	36.32025638615292	38.0	35.0	40.0	31.0	41.0
27	36.222888226221436	38.0	35.0	40.0	31.0	41.0
28	36.15864502494578	38.0	35.0	40.0	30.0	41.0
29	36.30287050479941	38.0	35.0	40.0	31.0	41.0
30	36.28101161815036	38.0	35.0	40.0	31.0	41.0
31	36.16755447857588	38.0	35.0	40.0	31.0	41.0
32	36.02647550857696	38.0	35.0	40.0	30.0	41.0
33	35.873347103121965	38.0	35.0	40.0	30.0	41.0
34	35.74740793738663	38.0	35.0	40.0	30.0	41.0
35	35.619348032713255	37.0	34.0	40.0	29.0	41.0
36	35.468116672411334	37.0	34.0	40.0	29.0	41.0
37	35.331048523272855	37.0	34.0	40.0	28.0	41.0
38	35.31606234528572	37.0	34.0	40.0	29.0	41.0
39	35.28273240652733	37.0	34.0	40.0	28.0	41.0
40	35.14487654192039	37.0	34.0	40.0	27.0	41.0
41	35.12491905244357	37.0	34.0	40.0	27.0	41.0
42	35.09091368031112	37.0	34.0	40.0	27.0	41.0
43	34.975676564899054	36.0	34.0	40.0	27.0	41.0
44	34.879441670757565	36.0	34.0	40.0	27.0	41.0
45	34.82804127803136	36.0	34.0	40.0	27.0	41.0
46	34.855697489407184	36.0	34.0	40.0	27.0	41.0
47	34.828017777127876	36.0	34.0	40.0	27.0	41.0
48	34.79590596853316	36.0	34.0	40.0	27.0	41.0
49	34.774588908269884	36.0	34.0	40.0	27.0	41.0
50	34.64237282529602	35.0	34.0	40.0	27.0	41.0
51	34.411912956135126	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	2.0
13	13.0
14	14.0
15	36.0
16	115.0
17	329.0
18	714.0
19	1485.0
20	2464.0
21	4268.0
22	6492.0
23	9847.0
24	14627.0
25	21423.0
26	29034.0
27	34557.0
28	38953.0
29	45298.0
30	56093.0
31	70663.0
32	90869.0
33	121465.0
34	217784.0
35	329701.0
36	166703.0
37	211966.0
38	309709.0
39	512902.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.395741288128036	24.226385073618754	29.165056419576423	13.21281721867678
2	29.233470160815813	27.039443220076386	30.30646048540681	13.420626133700992
3	28.741169753118655	25.679045549973367	31.467709758619613	14.11207493828837
4	24.645832680530457	28.71631972369901	31.287927847003893	15.349919748766638
5	22.65269494434638	33.12709114520773	30.335096771498105	13.885117138947786
6	21.409105468573202	41.44671561817821	26.4083569212772	10.735821991971395
7	89.99457738412315	3.7974413608938002	4.813768395985001	1.3942128589980607
8	91.96099372264756	2.278978354797492	4.290351051273749	1.4696768712811996
9	87.74349547215927	4.031710552427905	5.985593075763431	2.2392008996494015
10	49.963094877499366	24.54064437736532	13.974202971210522	11.522057773924791
11	43.043645529779994	23.078104817511132	19.409222102686762	14.46902755002211
12	37.47828342437757	22.667752930649705	23.71367369604802	16.140289948924703
13	22.12753679197	38.78314932996313	24.337187481503918	14.75212639656295
14	18.03394052704693	40.33960546335077	26.123865428604255	15.502588580998038
15	17.29792704623237	24.519928766150343	43.20319055228864	14.978953635328649
16	18.17372738255641	20.17657012147356	42.155311378267065	19.494391117702968
17	18.56540910720938	20.700118026759696	27.704866950070155	33.02960591596077
18	23.445763396385384	23.687300459921385	31.606452129530016	21.26048401416321
19	29.198131765213788	24.965096806314257	24.630295972119225	21.20647545635273
20	31.654280820129305	24.34014685453463	23.672285993809687	20.333286331526377
21	23.759108776107762	28.03048502383166	26.559807188143008	21.65059901191757
22	24.60113744372839	24.95103978441838	24.53494323226204	25.912879539591188
23	21.140150684311493	31.30307287369048	24.197226545227924	23.359549896770105
24	21.36545471637021	25.050744543438373	35.24465310925657	18.33914763093485
25	18.916965215181236	26.568467706277005	33.18149138474287	21.333075693798893
26	19.145446221228802	33.03691730815429	26.48952207866362	21.32811439195329
27	20.099713463058322	31.23344056708551	27.943139999234045	20.72370597062213
28	17.84306096656605	27.435346403317283	36.119234880215025	18.602357749901643
29	18.07297813893734	24.57271875859524	35.46643200579341	21.887871096674015
30	20.48730428969825	28.903848229424522	30.10605000295937	20.502797477917856
31	28.190291167490066	26.19393293712551	24.478192902378986	21.137582993005434
32	28.912421707175262	26.290243121198507	25.499959961423702	19.297375210202524
33	27.130139299429363	27.03826817490243	24.548478011858382	21.283114513809828
34	20.329456554663103	26.70742767814555	28.84257179961215	24.1205439675792
35	21.47455983678187	25.495172740344614	30.410952465505897	22.61931495736762
36	29.764590579445237	24.583555286310638	25.692145127653426	19.9597090065907
37	22.31254112658109	29.958864714873112	27.972907810307667	19.75568634823813
38	22.453111345539877	31.30968794281795	24.11075192446287	22.1264487871793
39	22.160829738565504	30.666241909596376	25.9613610330649	21.211567318773216
40	24.353115871639805	26.14714873112529	24.763163117159838	24.736572280075066
41	19.467321558510285	24.43193093867831	25.834064472552686	30.26668303025872
42	23.060783781243146	26.647369813698763	23.99442245224094	26.29742395281715
43	22.796833819018673	27.44909878387177	25.16289607726401	24.591171319845557
44	21.207084739035523	30.702233108072825	26.472723284695164	21.61795886819649
45	19.24985116094463	33.76078865550461	23.412035247873604	23.577324935677158
46	22.535625628866768	31.136259979179943	25.37788582390686	20.95022856804643
47	22.866988367923184	26.880507480250536	27.360535193908564	22.891968957917715
48	24.144088391250005	23.49829226768051	29.126541049985548	23.231078291083932
49	21.601638796335948	23.738958927383948	30.777479519397822	23.881922756882282
50	20.710736953516953	30.897986930016053	26.073643127465417	22.317632989001577
51	20.280888020806135	31.92628201780498	24.16563088610592	23.627199075282967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	458.0
1	1052.5
2	1647.0
3	4993.0
4	8339.0
5	6616.0
6	4893.0
7	5295.5
8	5698.0
9	6181.5
10	6665.0
11	6829.5
12	6994.0
13	6809.5
14	6625.0
15	6493.0
16	6361.0
17	6030.5
18	5700.0
19	5891.0
20	6082.0
21	6623.0
22	7164.0
23	7237.0
24	7310.0
25	8982.0
26	13214.5
27	15775.0
28	18394.0
29	21013.0
30	26233.5
31	31454.0
32	37593.5
33	43733.0
34	51542.5
35	59352.0
36	63066.0
37	66780.0
38	81950.0
39	97120.0
40	129050.5
41	160981.0
42	184761.0
43	208541.0
44	207845.5
45	207150.0
46	201075.5
47	195001.0
48	191656.0
49	188311.0
50	192221.0
51	196131.0
52	183278.0
53	170425.0
54	152260.5
55	134096.0
56	118610.0
57	103124.0
58	94332.0
59	85540.0
60	76047.5
61	66555.0
62	59431.5
63	52308.0
64	44081.0
65	35854.0
66	31439.0
67	27024.0
68	21995.5
69	16967.0
70	13749.0
71	10531.0
72	9149.5
73	7768.0
74	6519.5
75	4230.0
76	3189.0
77	2374.5
78	1560.0
79	1219.5
80	879.0
81	697.0
82	515.0
83	311.5
84	108.0
85	98.0
86	88.0
87	58.5
88	29.0
89	21.5
90	14.0
91	10.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2297784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.429310543125645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72576774830124	14.508616215794287
2	7.649792800170782	2.8196081420982804
3	2.3348631571374923	1.2908975459576884
4	1.186604674949544	0.8747322418627924
5	0.772858367617211	0.7121623431335371
6	0.5563932345596538	0.6152366222276405
7	0.44094048869659386	0.5688360438058978
8	0.3682566476327052	0.5429372895034815
9	0.31301649528463593	0.5191810377049241
>10	4.379832140322102	20.11172897044038
>50	2.1500136920412283	29.22657109052544
>100	1.1190454058147534	27.492433055485655
>500	0.0019019254342298863	0.20829160421245566
>1k	4.7548135855747157E-4	0.26165701732368224
>5k	2.3774067927873578E-4	0.24711077992390618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5640	0.24545388078252786	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4239	0.18448209231154886	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03829776863273485	0.0
2	0.0	0.0	0.0	0.129211448943852	0.0
3	0.0	0.0	0.0	0.19766871037486552	0.0
4	0.0	0.0	0.0	0.3185242825261208	0.0
5	0.0	0.0	0.0	0.5227210216452025	0.0
6	0.0	0.0	0.0	0.7968547087106534	0.0
7	0.0	0.0	0.0	0.9360322815373421	0.0
8	0.0	0.0	0.0	1.3459924866741173	0.0
9	0.0	0.0	0.0	1.5141545071251257	0.0
10	0.0	0.0	0.0	1.7863297855673117	0.0
11	0.0	0.0	0.0	2.0818318867221635	0.0
12	0.0	0.0	0.0	2.332725791458205	0.0
13	0.0	0.0	0.0	2.446095890649426	0.0
14	0.0	0.0	0.0	2.4936199399073193	0.0
15	0.0	0.0	0.0	2.555549172594117	0.0
16	0.0	0.0	0.0	2.7086532067418	0.0
17	0.0	0.0	0.0	2.8881304770161162	0.0
18	0.0	0.0	0.0	3.118178209962294	0.0
19	0.0	0.0	0.0	3.241427392653095	0.0
20	0.0	0.0	0.0	3.3720314877290467	0.0
21	0.0	0.0	0.0	3.5451112898340313	0.0
22	0.0	0.0	0.0	3.7235005553176452	0.0
23	0.0	0.0	0.0	3.9405357509670185	0.0
24	0.0	0.0	0.0	4.101212298457993	0.0
25	0.0	0.0	0.0	4.237996260745136	0.0
26	0.0	0.0	0.0	4.370906925977376	0.0
27	0.0	0.0	0.0	4.505775999832882	0.0
28	0.0	0.0	0.0	4.6459980572586455	0.0
29	0.0	0.0	0.0	4.799667853897494	0.0
30	0.0	0.0	0.0	4.992592863384896	0.0
31	0.0	0.0	0.0	5.167456993346633	0.0
32	0.0	0.0	0.0	5.337577422420907	0.0
33	0.0	0.0	0.0	5.505434801530518	0.0
34	0.0	0.0	0.0	5.692266984189985	0.0
35	0.0	0.0	0.0	5.90442791837701	0.0
36	0.0	0.0	0.0	6.089736894329493	0.0
37	0.0	0.0	0.0	6.275437552006629	0.0
38	0.0	0.0	0.0	6.466839354787047	0.0
39	0.0	0.0	0.0	6.652670573039067	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTAGCG	125	0.0	45.000004	1
AATCGGT	25	3.8920265E-5	45.000004	18
CGTCTAC	45	3.8562575E-10	45.0	41
TCTACGA	20	7.0347614E-4	45.0	39
TCGAACG	20	7.0347614E-4	45.0	1
TAATGCG	90	0.0	42.5	1
CGTTTTT	4815	0.0	41.682243	1
CGTTGAT	255	0.0	41.47059	25
CGAGACA	525	0.0	40.285713	22
GCGTAAG	190	0.0	40.263157	1
TACGGGA	700	0.0	39.214287	4
AATGCGG	425	0.0	39.176468	2
TATTCCG	75	0.0	39.0	1
GTTACGG	245	0.0	38.57143	2
TGCGTAG	105	0.0	38.57143	1
TATCTCG	35	6.251017E-6	38.571426	1
TGTTACG	100	0.0	38.250004	1
TATCACG	65	9.094947E-12	38.076927	1
CGTTAGG	325	0.0	38.076923	2
ACTACGG	160	0.0	37.96875	2
>>END_MODULE