FastQCFastQC Report
Thu 26 May 2016
SRR1545371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545371_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4946234
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142950.2890077582257532No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT69600.14071311628200364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC21950.039.77221327
AACACGT20750.039.57831241
TAACGCG403.4613186E-739.3750041
CGATCGC403.4613186E-739.37500410
CGTTTTT122900.039.19651
ACACGAC22750.038.76923426
GCTACGA10250.038.19512610
ACGACCA22800.038.1907928
GACCGAT30500.038.065579
CGTAAGG7350.037.9591832
CATACGA17150.037.91545518
AGACACG23150.037.80777424
TACGAAT10400.037.6442312
GACACGA23500.037.4361725
GCGATAT7350.037.3469359
GCGAGAC23650.037.2938721
TACGGGA14000.037.2857134
TAAGGGA52850.037.209084
CGAGACA23700.037.1202522
GGGCGAT126150.036.920337
Produced by FastQC (version 0.11.5)