##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545371_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4946234
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17622659987376	31.0	31.0	33.0	30.0	33.0
2	31.535360235686383	31.0	31.0	34.0	30.0	34.0
3	31.715451796255493	31.0	31.0	34.0	30.0	34.0
4	35.51186842353192	37.0	35.0	37.0	33.0	37.0
5	35.423069349327186	37.0	35.0	37.0	33.0	37.0
6	35.47690222500594	37.0	35.0	37.0	33.0	37.0
7	35.82989947503495	37.0	35.0	37.0	35.0	37.0
8	35.87212958384096	37.0	35.0	37.0	35.0	37.0
9	37.757667146358216	39.0	37.0	39.0	35.0	39.0
10	37.09140772555443	39.0	37.0	39.0	33.0	39.0
11	36.7599193649148	39.0	35.0	39.0	32.0	39.0
12	36.22492142506804	38.0	35.0	39.0	32.0	39.0
13	36.0023005381468	38.0	35.0	39.0	31.0	39.0
14	37.01369607665145	39.0	35.0	41.0	31.0	41.0
15	37.198306024340944	39.0	35.0	41.0	32.0	41.0
16	37.24001735461768	39.0	35.0	41.0	32.0	41.0
17	37.16078454840592	39.0	35.0	41.0	32.0	41.0
18	37.09913562520495	39.0	35.0	41.0	32.0	41.0
19	37.07842269492305	39.0	35.0	41.0	32.0	41.0
20	36.9696874430122	39.0	35.0	41.0	31.0	41.0
21	36.85312037400576	38.0	35.0	41.0	31.0	41.0
22	36.732048059190085	38.0	35.0	41.0	31.0	41.0
23	36.63028316088563	38.0	35.0	41.0	31.0	41.0
24	36.53614507522288	38.0	35.0	40.0	31.0	41.0
25	36.41618633489641	38.0	35.0	40.0	30.0	41.0
26	36.257027467766385	38.0	35.0	40.0	30.0	41.0
27	36.12799879666025	38.0	35.0	40.0	30.0	41.0
28	36.05051681744131	38.0	35.0	40.0	30.0	41.0
29	36.153977753579795	38.0	35.0	40.0	30.0	41.0
30	36.12855174259851	38.0	35.0	40.0	30.0	41.0
31	36.03459601789968	38.0	35.0	40.0	30.0	41.0
32	35.93111688609961	38.0	35.0	40.0	30.0	41.0
33	35.745373348693164	38.0	34.0	40.0	29.0	41.0
34	35.593492948372436	38.0	34.0	40.0	28.0	41.0
35	35.481827790597855	38.0	34.0	40.0	27.0	41.0
36	35.33468837099094	38.0	34.0	40.0	27.0	41.0
37	35.23585721985656	38.0	34.0	40.0	27.0	41.0
38	35.16098126372509	37.0	34.0	40.0	27.0	41.0
39	35.09260742617515	37.0	34.0	40.0	26.0	41.0
40	34.98390957645756	37.0	34.0	40.0	26.0	41.0
41	34.90903665293636	37.0	34.0	40.0	26.0	41.0
42	34.840443860925305	37.0	34.0	40.0	26.0	41.0
43	34.72098974694687	37.0	33.0	40.0	25.0	41.0
44	34.606953896641365	37.0	33.0	40.0	25.0	41.0
45	34.56448643553863	36.0	33.0	40.0	24.0	41.0
46	34.548889720947294	36.0	33.0	40.0	24.0	41.0
47	34.51260352826009	36.0	33.0	40.0	25.0	41.0
48	34.4334437068687	36.0	33.0	40.0	24.0	41.0
49	34.38040962073367	36.0	33.0	40.0	24.0	41.0
50	34.25909024926843	36.0	33.0	40.0	24.0	41.0
51	34.019628671025266	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	6.0
12	13.0
13	13.0
14	47.0
15	148.0
16	401.0
17	1152.0
18	2472.0
19	4686.0
20	8186.0
21	12988.0
22	19138.0
23	27347.0
24	38584.0
25	54066.0
26	70965.0
27	85233.0
28	97123.0
29	111372.0
30	133957.0
31	162564.0
32	200110.0
33	255942.0
34	412926.0
35	559671.0
36	387841.0
37	489921.0
38	693066.0
39	1115780.0
40	505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.77411299182368	23.816887757433232	28.050896904594484	13.358102346148607
2	31.26914335229591	25.790510517698923	29.43303935883341	13.507306771171764
3	29.81498651297128	25.808301831251818	30.753397433279538	13.62331422249736
4	26.602097676737497	28.114905198581386	30.1800925714392	15.102904553241922
5	24.078076370830818	32.36082239538202	28.378176204360734	15.182925029426428
6	22.23151998065599	41.3381776923615	25.769929202702503	10.660373124280008
7	89.41404713161569	3.6977223479519976	5.1907168160665265	1.6975137043657864
8	90.39077002826798	2.699852049053886	5.073152624805053	1.836225297873089
9	85.22659866071844	4.953728432581232	6.873957034786465	2.945715871913864
10	44.35513968809401	28.829388176944313	13.432259775821361	13.383212359140305
11	35.632564088152726	24.27974495343326	23.207575703050036	16.880115255363982
12	32.56592793628445	22.977865584200018	26.97913200224656	17.47707447726897
13	23.209819834645913	31.139125241547404	27.673781709478362	17.977273214328314
14	20.117264973715358	32.6019957810326	28.68020396932292	18.60053527592912
15	19.707680631365196	24.029635476202703	37.897600477454155	18.36508341497794
16	21.958342448011962	21.651523158831548	36.1576302293826	20.232504163773893
17	21.69114926629027	21.86974979347924	28.31841356474441	28.120687375486074
18	24.291875394492052	23.521531735053376	30.724830244586084	21.461762625868488
19	28.284387677574497	25.136133874782306	25.201395647678616	21.378082799964577
20	30.52457688010717	23.725687058072868	25.131160393948203	20.61857566787176
21	26.128727431819843	26.26780293855891	26.745277316034787	20.858192313586457
22	25.28873482330193	24.2511777647398	26.10026133013521	24.359826081823062
23	23.45485474403354	27.77484850089988	25.83094532122823	22.93935143383835
24	22.581341683389827	24.890290269324094	32.50719638415813	20.021171663127948
25	21.999161382174805	25.946750598536177	29.94294244873979	22.11114557054923
26	21.372199536051063	29.11829484816125	26.77196428636413	22.737541329423557
27	21.616547053778692	27.830587877565033	28.04869320780214	22.504171860854136
28	20.191907621030463	27.30952478188456	31.888099107320844	20.61046848976413
29	20.95400258054916	24.520817252075013	31.485954768820072	23.03922539855575
30	22.77185026021818	27.22841256600476	29.08182265537781	20.91791451839925
31	26.030491076645383	27.19123276415956	25.026090556977287	21.752185602217768
32	27.197540593510134	26.466802824128415	25.098569942303577	21.23708664005787
33	25.655498708714546	27.079289010588663	25.0376953455902	22.22751693510659
34	21.634176628117473	27.154113614519655	28.00011079136167	23.211598966001205
35	22.378884622118566	26.224234437756078	28.356017932026667	23.040863008098686
36	26.938292850681954	27.685083237064806	24.608722514947736	20.767901397305504
37	23.806233186703256	29.600722489069465	26.18375919942324	20.409285124804043
38	22.96818144875475	29.865833278409394	25.507345588583153	21.658639684252705
39	22.423625732223748	29.882957417704052	25.73111179131436	21.962305058757835
40	24.420902852554086	26.682845979385526	25.719183524273216	23.177067643787172
41	20.58667260788713	26.274292724525367	26.44167259373495	26.69736207385255
42	22.287360444329966	27.66529040073721	25.087672762752426	24.959676392180395
43	22.448250527573098	27.086486405616878	26.025153682579514	24.440109384230507
44	22.139955368063866	28.281051806283326	26.596780500073393	22.98221232557942
45	21.05557480701479	29.68430122796455	24.692786471485174	24.567337493535486
46	22.70606283487599	29.636507290192903	26.02096868041423	21.63646119451688
47	22.427992691004913	26.822083225338712	27.84259701421324	22.907327069443138
48	23.086938466720337	25.056659268445447	28.17549675167006	23.68090551316416
49	21.567762463320577	26.081560233502906	28.955706503169885	23.394970800006632
50	21.362394096195207	29.028610453933233	27.384470690226138	22.22452475964542
51	21.257122085206646	29.08083200269134	25.85708642170993	23.804959490392083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1247.0
1	3279.5
2	5312.0
3	16997.5
4	28683.0
5	21264.5
6	13846.0
7	13836.0
8	13826.0
9	14309.5
10	14793.0
11	14977.5
12	15162.0
13	15301.5
14	15441.0
15	15161.5
16	14882.0
17	14792.5
18	14703.0
19	14032.5
20	13362.0
21	14742.0
22	16122.0
23	18432.0
24	20742.0
25	25068.0
26	35476.0
27	41558.0
28	50959.5
29	60361.0
30	70728.0
31	81095.0
32	95114.0
33	109133.0
34	127249.0
35	145365.0
36	153357.0
37	161349.0
38	178104.0
39	194859.0
40	232465.0
41	270071.0
42	308975.0
43	347879.0
44	361627.5
45	375376.0
46	372415.0
47	369454.0
48	374171.0
49	378888.0
50	370090.5
51	361293.0
52	344645.0
53	327997.0
54	304448.0
55	280899.0
56	261815.5
57	242732.0
58	233821.0
59	224910.0
60	209713.5
61	194517.0
62	175237.0
63	155957.0
64	141181.0
65	126405.0
66	108137.0
67	89869.0
68	78723.5
69	67578.0
70	56546.5
71	45515.0
72	37769.0
73	30023.0
74	25038.5
75	16618.0
76	13182.0
77	9796.0
78	6410.0
79	4945.5
80	3481.0
81	2487.5
82	1494.0
83	993.5
84	493.0
85	359.0
86	225.0
87	174.0
88	123.0
89	114.0
90	105.0
91	71.0
92	37.0
93	32.0
94	27.0
95	16.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4946234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.879041460746652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95733134893376	13.49603110929311
2	7.647548179676606	2.58166565595638
3	2.45687570418194	1.2440912062436429
4	1.1560021654708355	0.7804883391878057
5	0.6877340151518553	0.5804145477856966
6	0.47142139845076075	0.4774284797960135
7	0.35690787893936227	0.421698402039925
8	0.2853902902634315	0.3853691633480785
9	0.23687797439620747	0.3598445835874142
>10	3.16660109548716	13.558415584149449
>50	1.8325383084576234	22.828680568023785
>100	1.7389615572690815	42.030614234470434
>500	0.003994432283459392	0.42498452581240814
>1k	0.0015735642328778003	0.3960134158848756
>5k	1.2104340252906157E-4	0.1421995240447162
>10k+	1.2104340252906157E-4	0.29206066037632444
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14295	0.2890077582257532	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6960	0.14071311628200364	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029375884764044725	0.0
2	0.0	0.0	0.0	0.09277765669800499	0.0
3	0.0	0.0	0.0	0.14877985958610127	0.0
4	0.0	0.0	0.0	0.25200991299643327	0.0
5	0.0	0.0	0.0	0.45030218950417633	0.0
6	0.0	0.0	0.0	0.7629845251963413	0.0
7	0.0	0.0	0.0	0.9345696139729742	0.0
8	0.0	0.0	0.0	1.3497946114154729	0.0
9	0.0	0.0	0.0	1.5624412431761214	0.0
10	0.0	0.0	0.0	1.8501550876889368	0.0
11	0.0	0.0	0.0	2.120016966443561	0.0
12	0.0	0.0	0.0	2.3456633875388833	0.0
13	0.0	0.0	0.0	2.4473367010133367	0.0
14	0.0	0.0	0.0	2.492118245922049	0.0
15	0.0	0.0	0.0	2.5506274066289625	0.0
16	0.0	0.0	0.0	2.6735289919563043	0.0
17	0.0	0.0	0.0	2.8151518913177176	0.0
18	0.0	0.0	0.0	2.9837448046331816	0.0
19	0.0	0.0	0.0	3.0842050740017557	0.0
20	0.0	0.0	0.0	3.1909933901226673	0.0
21	0.0	0.0	0.0	3.3333036811440784	0.0
22	0.0	0.0	0.0	3.4916868065683913	0.0
23	0.0	0.0	0.0	3.666931245064427	0.0
24	0.0	0.0	0.0	3.8061887084193753	0.0
25	0.0	0.0	0.0	3.9229442036102617	0.0
26	0.0	0.0	0.0	4.035433827028807	0.0
27	0.0	0.0	0.0	4.1495408425885225	0.0
28	0.0	0.0	0.0	4.278568300650556	0.0
29	0.0	0.0	0.0	4.411780760877872	0.0
30	0.0	0.0	0.0	4.582051718539803	0.0
31	2.0217401764655696E-5	0.0	0.0	4.7286076639317915	0.0
32	4.043480352931139E-5	0.0	0.0	4.877225784303775	0.0
33	4.043480352931139E-5	0.0	0.0	5.027319775004579	0.0
34	4.043480352931139E-5	0.0	0.0	5.17848528799891	0.0
35	4.043480352931139E-5	0.0	0.0	5.367720168516088	0.0
36	4.043480352931139E-5	0.0	0.0	5.524708293218639	0.0
37	4.043480352931139E-5	0.0	0.0	5.684587506373536	0.0
38	4.043480352931139E-5	0.0	0.0	5.850693679271947	0.0
39	4.043480352931139E-5	0.0	0.0	6.038816602692068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	2195	0.0	39.772213	27
AACACGT	2075	0.0	39.578312	41
TAACGCG	40	3.4613186E-7	39.375004	1
CGATCGC	40	3.4613186E-7	39.375004	10
CGTTTTT	12290	0.0	39.1965	1
ACACGAC	2275	0.0	38.769234	26
GCTACGA	1025	0.0	38.195126	10
ACGACCA	2280	0.0	38.19079	28
GACCGAT	3050	0.0	38.06557	9
CGTAAGG	735	0.0	37.959183	2
CATACGA	1715	0.0	37.915455	18
AGACACG	2315	0.0	37.807774	24
TACGAAT	1040	0.0	37.64423	12
GACACGA	2350	0.0	37.43617	25
GCGATAT	735	0.0	37.346935	9
GCGAGAC	2365	0.0	37.29387	21
TACGGGA	1400	0.0	37.285713	4
TAAGGGA	5285	0.0	37.20908	4
CGAGACA	2370	0.0	37.12025	22
GGGCGAT	12615	0.0	36.92033	7
>>END_MODULE