FastQCFastQC Report
Thu 26 May 2016
SRR1545370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545370_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3160669
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139230.44050800637459975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124300.041.0539051
TCGATAG950.040.2631571
ACGTTAG1450.038.7931021
TAGGGCG19550.038.554995
AGGGCGA41450.038.2689976
GGGCGAT78550.037.380657
AAGGGCG15600.036.9230775
TAGGGTA17750.036.6338045
GGCGATA17800.036.53098
TACGGGT4300.036.1046524
GGCGATT23400.036.057698
CGGTCTA1250.036.031
ACACGAC8950.035.94972226
GTAGGGC20600.035.825244
GCGATAA4150.035.7831349
CGTAAGG5350.035.747662
CACGACC8950.035.69832627
ACGACCA8900.035.6460728
CGAGGGT5600.035.3571434
TAGGGTC13050.035.3448265
Produced by FastQC (version 0.11.5)