##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545370_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3160669
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14794209706869	31.0	31.0	33.0	30.0	33.0
2	31.50558378621741	31.0	31.0	34.0	30.0	34.0
3	31.656894473923085	31.0	31.0	34.0	30.0	34.0
4	35.485039401468484	37.0	35.0	37.0	33.0	37.0
5	35.404918705501906	37.0	35.0	37.0	33.0	37.0
6	35.4677557188051	37.0	35.0	37.0	33.0	37.0
7	35.80758092669622	37.0	35.0	37.0	35.0	37.0
8	35.843429033536886	37.0	35.0	37.0	35.0	37.0
9	37.7458490591707	39.0	37.0	39.0	35.0	39.0
10	37.06449489016408	39.0	37.0	39.0	33.0	39.0
11	36.74561841179826	39.0	35.0	39.0	32.0	39.0
12	36.23090428007488	38.0	35.0	39.0	32.0	39.0
13	36.03201663951524	38.0	35.0	39.0	31.0	39.0
14	37.050701607792526	39.0	35.0	41.0	31.0	41.0
15	37.21722964347105	39.0	35.0	41.0	32.0	41.0
16	37.25103546116345	39.0	35.0	41.0	32.0	41.0
17	37.16254565093656	39.0	35.0	41.0	32.0	41.0
18	37.0758035719653	39.0	35.0	41.0	32.0	41.0
19	37.02736414347722	39.0	35.0	41.0	31.0	41.0
20	36.89442899588663	38.0	35.0	41.0	31.0	41.0
21	36.78947020393468	38.0	35.0	41.0	31.0	41.0
22	36.66503705386423	38.0	35.0	41.0	31.0	41.0
23	36.555727917096036	38.0	35.0	40.0	31.0	41.0
24	36.45960364720254	38.0	35.0	40.0	31.0	41.0
25	36.330205092656016	38.0	35.0	40.0	30.0	41.0
26	36.174476985726756	38.0	35.0	40.0	30.0	41.0
27	36.03587246877164	38.0	35.0	40.0	30.0	41.0
28	35.9354544876417	38.0	35.0	40.0	30.0	41.0
29	36.03327618298531	38.0	35.0	40.0	30.0	41.0
30	35.98966263155047	38.0	35.0	40.0	30.0	41.0
31	35.90348150976897	38.0	35.0	40.0	30.0	41.0
32	35.7544159163772	38.0	34.0	40.0	29.0	41.0
33	35.534900997225584	38.0	34.0	40.0	28.0	41.0
34	35.32402159163139	38.0	34.0	40.0	27.0	41.0
35	35.159060629252856	38.0	34.0	40.0	26.0	41.0
36	35.01658952582507	38.0	34.0	40.0	25.0	41.0
37	34.90877690767366	37.0	34.0	40.0	25.0	41.0
38	34.82162510531789	37.0	33.0	40.0	25.0	41.0
39	34.739590890409595	37.0	33.0	40.0	24.0	41.0
40	34.606527921778586	37.0	33.0	40.0	24.0	41.0
41	34.51718639313386	37.0	33.0	40.0	24.0	41.0
42	34.44294926169112	37.0	33.0	40.0	24.0	41.0
43	34.330971702509814	36.0	33.0	40.0	23.0	41.0
44	34.209312015905496	36.0	33.0	40.0	23.0	41.0
45	34.173398416601046	36.0	33.0	40.0	23.0	41.0
46	34.16832892023809	36.0	33.0	40.0	23.0	41.0
47	34.12377980737622	36.0	33.0	40.0	23.0	41.0
48	34.0291631297045	36.0	33.0	40.0	23.0	41.0
49	33.96744803078082	36.0	33.0	40.0	23.0	41.0
50	33.857748470339665	35.0	33.0	40.0	23.0	41.0
51	33.61111967118354	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	9.0
12	5.0
13	33.0
14	34.0
15	129.0
16	284.0
17	729.0
18	1697.0
19	3244.0
20	5637.0
21	8960.0
22	13354.0
23	19437.0
24	27694.0
25	39949.0
26	52819.0
27	61412.0
28	67248.0
29	75603.0
30	88498.0
31	105984.0
32	130295.0
33	165429.0
34	261915.0
35	343064.0
36	253628.0
37	318616.0
38	445956.0
39	668736.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.36721782635259	23.679290681814514	27.18490927079046	14.768582221042445
2	32.14705494311489	24.97189677248709	29.411906150248573	13.469142134149447
3	29.010219039070527	25.30521228258954	31.847371553300903	13.837197125039037
4	26.294401596624006	27.824773805798706	30.875551979660003	15.005272617917283
5	23.1905017576975	32.46100746392615	29.850705657568067	14.497785120808285
6	21.419674125952447	40.98682905422871	26.89870404018896	10.694792779629882
7	87.86823295954116	3.6126212520197467	6.753380376116576	1.765765412322518
8	88.75437446945568	2.8236110772750957	6.570476060606156	1.8515383926630724
9	84.32414150295396	4.235210963248603	8.352219102981046	3.0884284308163874
10	44.82626937524935	23.279913208248	17.079390470814882	14.814426945687764
11	36.51881927528634	25.20260742266906	21.379271287186352	16.899302014858247
12	32.07121656839106	22.849466362975686	26.942017655123013	18.13729941351024
13	23.73962600955684	29.24225852185091	28.170238642515237	18.847876826077012
14	20.875675371258424	31.081394476928775	29.10810970715377	18.934820444659024
15	20.66708029217865	23.882348958400897	36.85203986877462	18.59853088064584
16	22.456068636102042	20.66154348968525	36.21217533376637	20.670212540446343
17	22.407471329645716	21.81364135251113	28.665418618653206	27.11346869918995
18	24.51395574797614	23.797904810658757	29.96792767607111	21.720211765293993
19	28.497384572696475	24.945509953747134	25.269017413718426	21.288088059837964
20	30.031426890952517	23.79806300501571	25.205739670936754	20.96477043309502
21	26.574120858590383	25.37228668993811	26.860389366934655	21.193203084536847
22	25.44698606529187	23.90003508750837	26.30171017591529	24.351268671284465
23	24.356963668134814	26.871146583207544	25.71490402822947	23.056985720428173
24	23.4321594573807	24.56008522246398	31.417778957556138	20.58997636259918
25	23.030219235231527	24.986767042040782	29.261526594527933	22.721487128199758
26	22.321476877205427	28.033305607135706	26.77271805431065	22.872499461348212
27	22.68174870573287	27.238632074412095	27.55869089740178	22.520928322453255
28	20.679704201863593	27.289127713151867	31.09053811076073	20.94062997422381
29	22.103073747994493	24.98214776681772	30.36224293021509	22.552535554972696
30	22.826021959275078	26.499990982921656	28.860060955449622	21.813926102353648
31	26.471136332213213	25.471696023848118	25.05561322618724	23.001554417751432
32	27.110684478507558	25.771189580433763	25.32599902109332	21.792126919965362
33	25.580628658046763	25.83288537964589	25.25813364195998	23.32835232034737
34	22.450278722637517	26.184140129826943	27.488389325171347	23.87719182236419
35	22.899329224287644	25.604104700618763	28.141004325350107	23.35556174974349
36	26.8353946585359	26.724753525282146	24.579353295140997	21.86049852104096
37	23.978942432757115	28.34744796117531	25.995888845051475	21.6777207610161
38	23.582950318429422	28.924129670016065	24.599380700731395	22.89353931082312
39	23.467626632209825	28.443788324560405	25.08630293143635	23.00228211179342
40	24.563217470731672	25.493210456393882	26.04603645620595	23.8975356166685
41	21.551671497395013	25.4793526307247	26.683021853917637	26.285954017962652
42	23.5374536213694	27.15501053732612	24.378414822937803	24.929121018366683
43	23.288329148037963	26.091121847937888	26.21233036423618	24.408218639787968
44	23.011489023368153	27.509808841102945	26.36729755630849	23.111404579220412
45	21.948929166578342	28.7558741519596	24.855623920125772	24.439572761336287
46	23.33183259620036	28.15783620493003	25.634920961353437	22.87541023751617
47	23.19350745047963	26.547892234207378	27.172443555462465	23.086156759850525
48	23.91775285548724	24.471559660312423	27.817908170706897	23.79277931349344
49	22.34197886586669	25.35365139468891	27.809840258502234	24.494529480942166
50	21.727077400385806	27.35515803774454	27.283179605330393	23.634584956539264
51	21.726096595372688	28.45334326372043	25.502449006840006	24.318111134066868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	888.0
1	1989.5
2	3091.0
3	14886.0
4	26681.0
5	19560.5
6	12440.0
7	12287.5
8	12135.0
9	12337.0
10	12539.0
11	12799.0
12	13059.0
13	12991.5
14	12924.0
15	12468.0
16	12012.0
17	11804.5
18	11597.0
19	11351.0
20	11105.0
21	10994.0
22	10883.0
23	12231.5
24	13580.0
25	13999.5
26	17723.0
27	21027.0
28	24058.5
29	27090.0
30	32922.0
31	38754.0
32	46863.0
33	54972.0
34	61473.5
35	67975.0
36	77361.0
37	86747.0
38	98163.0
39	109579.0
40	131259.0
41	152939.0
42	176835.0
43	200731.0
44	213011.5
45	225292.0
46	229405.5
47	233519.0
48	235214.5
49	236910.0
50	234656.5
51	232403.0
52	225691.5
53	218980.0
54	203371.0
55	187762.0
56	179721.0
57	171680.0
58	167246.5
59	162813.0
60	155900.0
61	148987.0
62	133913.5
63	118840.0
64	106694.0
65	94548.0
66	82431.5
67	70315.0
68	58383.0
69	46451.0
70	39114.5
71	31778.0
72	26791.5
73	21805.0
74	17757.0
75	10883.5
76	8058.0
77	6646.5
78	5235.0
79	3787.5
80	2340.0
81	1798.0
82	1256.0
83	849.0
84	442.0
85	286.5
86	131.0
87	109.0
88	87.0
89	90.0
90	93.0
91	65.0
92	37.0
93	27.0
94	17.0
95	12.0
96	7.0
97	6.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3160669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.667683275181744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33434313145298	16.189893934102788
2	6.7231080714806755	2.779021364923611
3	2.2023780813478195	1.3655415791249756
4	1.1954177063883238	0.9882605814871234
5	0.8023481641311743	0.8291338866343322
6	0.6270253763672102	0.7775497130555478
7	0.5279796395060934	0.7638481175539601
8	0.42931687353598097	0.7098388133546327
9	0.37289284331532635	0.6936148063100823
>10	6.109503575633224	32.122259403181424
>50	2.383648167110377	34.03569401290325
>100	0.2883192642222068	7.672144137080508
>500	0.0029442918609299397	0.3785936036900766
>1k	6.198509180905136E-4	0.24869125561741348
>5k	0.0	0.0
>10k+	1.549627295226284E-4	0.4459147909801993
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13923	0.44050800637459975	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02701959616777334	0.0
2	0.0	0.0	0.0	0.08602609131168117	0.0
3	0.0	0.0	0.0	0.14607666921148654	0.0
4	0.0	0.0	0.0	0.25434488711092496	0.0
5	0.0	0.0	0.0	0.46816037997018983	0.0
6	0.0	0.0	0.0	0.868455380807038	0.0
7	0.0	0.0	0.0	1.104924305582141	0.0
8	0.0	0.0	0.0	1.6309838201975595	0.0
9	0.0	0.0	0.0	1.9320909592241389	0.0
10	0.0	0.0	0.0	2.2820168768067775	0.0
11	0.0	0.0	0.0	2.5634129989568666	0.0
12	0.0	0.0	0.0	2.796718036592886	0.0
13	0.0	0.0	0.0	2.91694574787806	0.0
14	0.0	0.0	0.0	2.977407631105946	0.0
15	0.0	0.0	0.0	3.044513677325908	0.0
16	0.0	0.0	0.0	3.161893890185907	0.0
17	0.0	0.0	0.0	3.2968653155392103	0.0
18	0.0	0.0	0.0	3.462494807270233	3.163887139083529E-5
19	0.0	0.0	0.0	3.5616826690805015	3.163887139083529E-5
20	0.0	0.0	0.0	3.6714695528067	3.163887139083529E-5
21	0.0	0.0	0.0	3.807516699787292	3.163887139083529E-5
22	0.0	0.0	0.0	3.957706422279587	3.163887139083529E-5
23	0.0	0.0	0.0	4.124728024351807	3.163887139083529E-5
24	0.0	0.0	0.0	4.264382002670954	3.163887139083529E-5
25	0.0	0.0	0.0	4.383723825557184	3.163887139083529E-5
26	0.0	0.0	0.0	4.504584314270175	3.163887139083529E-5
27	0.0	0.0	0.0	4.619528334033079	3.163887139083529E-5
28	0.0	0.0	0.0	4.745324486683041	3.163887139083529E-5
29	0.0011073604986792352	0.0	0.0	4.881150161563896	3.163887139083529E-5
30	0.0011073604986792352	0.0	0.0	5.052348094659706	3.163887139083529E-5
31	0.0011073604986792352	0.0	0.0	5.202221428438094	3.163887139083529E-5
32	0.0011073604986792352	0.0	0.0	5.3601310355497525	3.163887139083529E-5
33	0.0011073604986792352	0.0	0.0	5.513516284052522	3.163887139083529E-5
34	0.0011073604986792352	0.0	0.0	5.671141141321663	3.163887139083529E-5
35	0.0011073604986792352	0.0	0.0	5.849932403551273	3.163887139083529E-5
36	0.0011073604986792352	0.0	0.0	6.014960756725871	3.163887139083529E-5
37	0.0011073604986792352	0.0	0.0	6.185146245937173	3.163887139083529E-5
38	0.0011073604986792352	0.0	0.0	6.368588422261237	3.163887139083529E-5
39	0.0011073604986792352	0.0	0.0	6.560288344018308	3.163887139083529E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12430	0.0	41.053905	1
TCGATAG	95	0.0	40.263157	1
ACGTTAG	145	0.0	38.793102	1
TAGGGCG	1955	0.0	38.55499	5
AGGGCGA	4145	0.0	38.268997	6
GGGCGAT	7855	0.0	37.38065	7
AAGGGCG	1560	0.0	36.923077	5
TAGGGTA	1775	0.0	36.633804	5
GGCGATA	1780	0.0	36.5309	8
TACGGGT	430	0.0	36.104652	4
GGCGATT	2340	0.0	36.05769	8
CGGTCTA	125	0.0	36.0	31
ACACGAC	895	0.0	35.949722	26
GTAGGGC	2060	0.0	35.82524	4
GCGATAA	415	0.0	35.783134	9
CGTAAGG	535	0.0	35.74766	2
CACGACC	895	0.0	35.698326	27
ACGACCA	890	0.0	35.64607	28
CGAGGGT	560	0.0	35.357143	4
TAGGGTC	1305	0.0	35.344826	5
>>END_MODULE