FastQCFastQC Report
Thu 26 May 2016
SRR1545369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545369_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5762944
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC154150.2674848133176377No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95700.1660609577327144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAA600.045.00000433
GTCGAAC207.035647E-445.028
CGTAAGG9550.041.2303622
TAGTACG1750.041.142861
CGTTTTT107900.041.0588531
ACGTAAG2050.040.6097561
TCGTTAC950.040.26315737
ACTACGC451.9306754E-840.014
GTATCGC451.9306754E-840.01
CACGACC11700.039.8076927
AGGGCGA63550.039.4767956
CATCGCA800.039.37523
TCGTCAG1150.039.1304361
TCGAACG1150.039.1304361
CGTAATC750.039.016
GGCGATA25000.038.978
ACACGAC12300.038.96341326
TCGCTAC356.252654E-638.5714339
CGTCGTT356.252654E-638.5714324
GGGCGAT131900.038.3472337
Produced by FastQC (version 0.11.5)