##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545368_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	55875
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236706935123042	31.0	31.0	33.0	30.0	34.0
2	31.615212527964207	31.0	31.0	34.0	30.0	34.0
3	31.79856823266219	31.0	31.0	34.0	30.0	34.0
4	35.57950782997763	37.0	35.0	37.0	33.0	37.0
5	35.48771364653244	37.0	35.0	37.0	33.0	37.0
6	35.54652348993289	37.0	35.0	37.0	33.0	37.0
7	35.865557046979866	37.0	35.0	37.0	35.0	37.0
8	35.91169574944072	37.0	35.0	37.0	35.0	37.0
9	37.799284116331094	39.0	37.0	39.0	35.0	39.0
10	37.16978970917226	39.0	37.0	39.0	33.0	39.0
11	36.82480536912752	39.0	35.0	39.0	32.0	39.0
12	36.023624161073826	37.0	35.0	39.0	32.0	39.0
13	35.67336017897092	37.0	35.0	39.0	31.0	39.0
14	36.637351230425054	38.0	35.0	41.0	31.0	41.0
15	36.926335570469796	38.0	35.0	41.0	32.0	41.0
16	37.0255033557047	38.0	35.0	41.0	32.0	41.0
17	36.999570469798655	38.0	35.0	41.0	32.0	41.0
18	36.94663087248322	38.0	35.0	41.0	32.0	41.0
19	36.872984340044745	38.0	35.0	41.0	32.0	41.0
20	36.75772706935123	38.0	35.0	41.0	32.0	41.0
21	36.646854586129756	38.0	35.0	41.0	31.0	41.0
22	36.51133780760627	38.0	35.0	40.0	31.0	41.0
23	36.45385234899329	38.0	35.0	40.0	31.0	41.0
24	36.33313646532439	38.0	35.0	40.0	31.0	41.0
25	36.19606263982103	38.0	35.0	40.0	31.0	41.0
26	36.051919463087245	38.0	35.0	40.0	30.0	41.0
27	35.915901565995526	37.0	35.0	40.0	30.0	41.0
28	35.846425055928414	37.0	35.0	40.0	30.0	41.0
29	35.98478747203579	37.0	35.0	40.0	30.0	41.0
30	36.02920805369128	37.0	35.0	40.0	30.0	41.0
31	35.918156599552574	37.0	35.0	40.0	30.0	41.0
32	35.78505592841163	37.0	34.0	40.0	30.0	41.0
33	35.605780760626395	37.0	34.0	40.0	29.0	41.0
34	35.47371812080537	37.0	34.0	40.0	29.0	41.0
35	35.35847874720358	37.0	34.0	40.0	29.0	41.0
36	35.17929306487696	37.0	34.0	40.0	27.0	41.0
37	35.06686353467561	37.0	34.0	40.0	27.0	41.0
38	35.01791498881432	37.0	34.0	40.0	27.0	41.0
39	34.982156599552575	36.0	34.0	40.0	27.0	41.0
40	34.80103803131991	36.0	34.0	40.0	26.0	41.0
41	34.755328859060405	36.0	34.0	40.0	26.0	41.0
42	34.699847874720355	36.0	33.0	40.0	26.0	41.0
43	34.60656823266219	36.0	33.0	40.0	26.0	41.0
44	34.49308277404922	36.0	33.0	40.0	25.0	41.0
45	34.47069351230425	35.0	33.0	40.0	25.0	41.0
46	34.469261744966445	35.0	33.0	40.0	26.0	41.0
47	34.43665324384787	35.0	33.0	40.0	26.0	41.0
48	34.369324384787475	35.0	33.0	40.0	25.0	41.0
49	34.36144966442953	35.0	33.0	40.0	26.0	41.0
50	34.26774049217002	35.0	33.0	40.0	26.0	41.0
51	34.03151677852349	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	4.0
17	9.0
18	25.0
19	54.0
20	90.0
21	141.0
22	200.0
23	299.0
24	408.0
25	579.0
26	750.0
27	961.0
28	1111.0
29	1167.0
30	1509.0
31	1908.0
32	2343.0
33	3069.0
34	5292.0
35	7868.0
36	4075.0
37	5089.0
38	7140.0
39	11777.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.490827740492165	23.59552572706935	28.842953020134228	14.07069351230425
2	29.61073825503356	26.00447427293065	30.553914988814316	13.830872483221476
3	30.04384787472036	25.789709172259506	30.149440715883667	14.017002237136467
4	26.573601789709173	27.983892617449662	29.897091722595075	15.545413870246087
5	23.513199105145414	32.893064876957496	28.553020134228184	15.040715883668904
6	22.25682326621924	41.68769574944072	25.401342281879195	10.65413870246085
7	89.44250559284116	3.8156599552572708	5.1579418344519015	1.5838926174496646
8	90.97986577181209	2.7883668903803134	4.676510067114094	1.5552572706935124
9	86.07069351230425	4.751677852348993	6.444742729306488	2.732885906040268
10	51.038926174496645	24.477852348993288	12.540492170022372	11.942729306487696
11	43.225055928411635	21.75391498881432	19.89082774049217	15.130201342281879
12	38.98523489932886	22.14765100671141	23.801342281879194	15.065771812080536
13	22.480536912751678	37.007606263982105	24.28993288590604	16.22192393736018
14	18.503803131991052	38.86353467561521	26.421476510067116	16.211185682326622
15	17.281431767337807	23.93557046979866	42.93691275167785	15.846085011185682
16	18.890380313199103	19.001342281879193	42.41073825503356	19.697539149888144
17	19.59910514541387	20.13959731543624	27.44876957494407	32.81252796420582
18	24.10559284116331	24.44384787472036	29.861297539149888	21.589261744966443
19	29.247427293064877	24.565548098434004	24.17897091722595	22.00805369127517
20	32.67293064876958	23.02281879194631	23.35212527964206	20.952125279642058
21	25.43534675615213	25.904250559284115	25.972259507829975	22.68814317673378
22	25.331543624161075	24.86979865771812	24.47427293064877	25.324384787472038
23	23.108724832214765	28.975391498881432	24.014317673378077	23.90156599552573
24	22.79194630872483	24.848322147651007	33.106040268456375	19.253691275167785
25	21.04876957494407	25.734228187919463	30.577181208053688	22.639821029082775
26	20.32393736017897	31.328859060402685	25.41208053691275	22.93512304250559
27	21.45145413870246	29.78255033557047	27.454138702460853	21.311856823266222
28	17.839821029082774	28.157494407158833	34.44653243847875	19.55615212527964
29	18.75436241610738	24.75704697986577	33.95436241610738	22.534228187919464
30	21.100671140939596	27.513199105145414	30.208501118568233	21.177628635346757
31	27.52572706935123	26.75078299776286	23.869351230425057	21.85413870246085
32	28.982550335570465	26.54317673378076	24.740939597315435	19.733333333333334
33	26.32304250559284	27.78523489932886	24.75883668903803	21.13288590604027
34	21.535570469798657	26.732885906040266	28.55659955257271	23.174944071588367
35	22.462639821029082	26.14407158836689	28.182550335570472	23.210738255033558
36	28.808948545861295	25.327964205816556	25.057718120805365	20.80536912751678
37	23.733333333333334	29.188366890380312	27.706487695749445	19.371812080536913
38	23.08545861297539	29.882774049217005	23.71185682326622	23.319910514541387
39	22.62371364653244	29.285011185682325	26.37136465324385	21.71991051454139
40	24.304250559284117	25.58926174496644	25.596420581655483	24.510067114093957
41	19.23758389261745	25.075615212527964	26.521700223713644	29.16510067114094
42	23.625950782997762	26.791946308724835	23.889038031319913	25.693064876957493
43	23.844295302013425	27.518568232662194	24.769574944071586	23.867561521252796
44	22.224608501118567	29.771812080536915	25.807606263982102	22.195973154362417
45	19.912304250559284	32.31498881431767	22.638031319910514	25.134675615212526
46	22.93512304250559	30.41968680089486	25.195525727069352	21.449664429530202
47	24.051901565995525	26.55749440715884	27.037136465324384	22.353467561521253
48	25.5248322147651	22.87606263982103	27.835346756152124	23.763758389261742
49	21.406711409395974	24.374049217002238	29.18657718120805	25.032662192393733
50	20.561968680089485	29.78434004474273	26.627293064876955	23.02639821029083
51	20.465324384787472	31.260850111856826	23.754809843400448	24.519015659955258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	28.0
2	46.0
3	148.5
4	251.0
5	193.0
6	135.0
7	151.5
8	168.0
9	175.5
10	183.0
11	171.0
12	159.0
13	152.0
14	145.0
15	150.0
16	155.0
17	143.0
18	131.0
19	147.0
20	163.0
21	176.0
22	189.0
23	188.0
24	187.0
25	214.5
26	303.5
27	365.0
28	451.5
29	538.0
30	625.5
31	713.0
32	863.5
33	1014.0
34	1225.5
35	1437.0
36	1474.0
37	1511.0
38	1706.5
39	1902.0
40	2645.5
41	3389.0
42	4067.5
43	4746.0
44	4692.5
45	4639.0
46	4758.5
47	4878.0
48	4603.5
49	4329.0
50	4140.5
51	3952.0
52	3790.0
53	3628.0
54	3386.5
55	3145.0
56	2984.5
57	2824.0
58	2684.5
59	2545.0
60	2395.5
61	2246.0
62	2038.5
63	1831.0
64	1582.5
65	1334.0
66	1140.0
67	946.0
68	784.5
69	623.0
70	551.0
71	479.0
72	366.5
73	254.0
74	232.5
75	160.0
76	109.0
77	84.0
78	59.0
79	46.0
80	33.0
81	26.0
82	19.0
83	11.0
84	3.0
85	4.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	55875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.374049217002245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.39248865043334	35.73691275167785
2	16.65449696815772	18.77762863534676
3	9.63840121908632	16.300671140939595
4	5.25413505190641	11.847874720357941
5	2.8064382996285597	7.910514541387024
6	1.2286104320772089	4.155704697986577
7	0.5365249690466364	2.117225950782998
8	0.2254039810787644	1.0165548098434003
9	0.12381345439537762	0.6281879194630873
>10	0.13333756627194515	1.0684563758389263
>50	0.0	0.0
>100	0.006349407917711674	0.44026845637583895
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	125	0.22371364653243847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.21655480984340048	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042953020134228186	0.0
2	0.0	0.0	0.0	0.14675615212527965	0.0
3	0.0	0.0	0.0	0.2076062639821029	0.0
4	0.0	0.0	0.0	0.2917225950782998	0.0
5	0.0	0.0	0.0	0.4868008948545861	0.0
6	0.0	0.0	0.0	0.7158836689038032	0.0
7	0.0	0.0	0.0	0.8519015659955257	0.0
8	0.0	0.0	0.0	1.1758389261744966	0.0
9	0.0	0.0	0.0	1.3404921700223713	0.0
10	0.0	0.0	0.0	1.5427293064876957	0.0
11	0.0	0.0	0.0	1.7539149888143177	0.0
12	0.0	0.0	0.0	1.934675615212528	0.0
13	0.0	0.0	0.0	2.0384787472035795	0.0
14	0.0	0.0	0.0	2.0814317673378078	0.0
15	0.0	0.0	0.0	2.1476510067114094	0.0
16	0.0	0.0	0.0	2.2532438478747205	0.0
17	0.0	0.0	0.0	2.396420581655481	0.0
18	0.0	0.0	0.0	2.589709172259508	0.0
19	0.0	0.0	0.0	2.6630872483221477	0.0
20	0.0	0.0	0.0	2.759731543624161	0.0
21	0.0	0.0	0.0	2.883221476510067	0.0
22	0.0	0.0	0.0	3.0156599552572705	0.0
23	0.0	0.0	0.0	3.2143176733780763	0.0
24	0.0	0.0	0.0	3.3181208053691273	0.0
25	0.0	0.0	0.0	3.4237136465324385	0.0
26	0.0	0.0	0.0	3.520357941834452	0.0
27	0.0	0.0	0.0	3.6241610738255035	0.0
28	0.0	0.0	0.0	3.7476510067114095	0.0
29	0.0	0.0	0.0	3.846085011185682	0.0
30	0.0	0.0	0.0	4.0	0.0
31	0.0	0.0	0.0	4.127069351230425	0.0
32	0.0	0.0	0.0	4.221923937360179	0.0
33	0.0	0.0	0.0	4.350782997762863	0.0
34	0.0	0.0	0.0	4.4724832214765105	0.0
35	0.0	0.0	0.0	4.624608501118568	0.0
36	0.0	0.0	0.0	4.755257270693512	0.0
37	0.0	0.0	0.0	4.90738255033557	0.0
38	0.0	0.0	0.0	5.064876957494407	0.0
39	0.0	0.0	0.0	5.204474272930649	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGCC	60	0.0	45.000004	8
CTTTAGG	20	6.975729E-4	45.000004	2
CACGCCA	40	6.6174835E-9	45.000004	27
AGGGTAC	20	6.975729E-4	45.000004	6
ATGCGGG	20	6.975729E-4	45.000004	3
GAGCTAG	20	6.975729E-4	45.000004	45
AAGGGTT	20	6.975729E-4	45.000004	5
AAGGGTC	20	6.975729E-4	45.000004	5
AAGGGCC	20	6.975729E-4	45.000004	5
TAGTTGG	20	6.975729E-4	45.000004	2
AAGGGAC	30	2.1284995E-6	45.000004	5
ACTTCCC	40	6.6174835E-9	45.000004	44
CATAGTT	20	6.975729E-4	45.000004	36
GGTAGGG	20	6.975729E-4	45.000004	3
CATAGGG	20	6.975729E-4	45.000004	3
GGGACCT	20	6.975729E-4	45.000004	7
TAATAGG	30	2.1284995E-6	45.000004	2
GTAGGGT	20	6.975729E-4	45.000004	4
CAAGGGA	30	2.1284995E-6	45.000004	4
TAGGGAA	40	6.6174835E-9	45.000004	5
>>END_MODULE