FastQCFastQC Report
Thu 26 May 2016
SRR1545367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545367_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4865657
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC112470.23115069557924037No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109130.22428625774484312No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT49720.10218558357072847No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.035538E-445.031
TACGTAG2600.042.403851
TTATTCG603.6379788E-1241.25000418
CGGGTAT4450.040.955066
GTCGAAC556.184564E-1140.90909229
CTCGTTA451.9304935E-840.024
TTACGTA451.9304935E-840.012
CGACTTA451.9304935E-840.027
GCTACGA7200.039.062510
TATAGCG2650.039.0566021
CGTAAGG6800.038.7132342
ACTATCG356.252454E-638.571432
TAGACGC356.252454E-638.5714335
CGCATGG7550.038.4437072
CGTTAGG7650.038.2352942
AACGGGA10850.038.156684
TAAACGG2300.038.1521762
TAATACG1300.038.0769271
CGTTTTT95600.038.0099331
AGTACGG3750.037.82
Produced by FastQC (version 0.11.5)