FastQCFastQC Report
Thu 26 May 2016
SRR1545366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545366_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2237299
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60700.2713092885662578No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43100.1926430039078371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA302.1664273E-645.00000419
ATTACGC551.8189894E-1245.00000422
CGTCGGA302.1664273E-645.00000423
TACGTCA302.1664273E-645.00000427
AATCCGT207.03472E-445.035
TCGTTAG850.045.01
GTACCGA253.8919934E-545.03
CGTATAT207.03472E-445.044
ACGCATA207.03472E-445.033
CTATACG207.03472E-445.01
ACTTACG253.8919934E-545.041
CTAACGA406.8193913E-945.038
CGTAGGT207.03472E-445.040
CGTAAGT253.8919934E-545.017
CCGGTAA207.03472E-445.029
GCGTAAG1500.045.01
TACCGGT253.8919934E-545.040
TATCCGA207.03472E-445.023
ACGTTAG1400.043.3928571
TACGTAG1450.041.896551