##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545366_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2237299
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31725620938462	31.0	31.0	33.0	30.0	34.0
2	31.681941036937843	31.0	31.0	34.0	30.0	34.0
3	31.874511185138868	31.0	31.0	34.0	30.0	34.0
4	35.63695777810655	37.0	35.0	37.0	33.0	37.0
5	35.562472427690714	37.0	35.0	37.0	33.0	37.0
6	35.614507940154624	37.0	35.0	37.0	33.0	37.0
7	35.92057208267648	37.0	35.0	37.0	35.0	37.0
8	35.96842263819007	37.0	35.0	37.0	35.0	37.0
9	37.90493000712019	39.0	38.0	39.0	35.0	39.0
10	37.31852827896495	39.0	37.0	39.0	34.0	39.0
11	36.912464985681396	39.0	37.0	39.0	33.0	39.0
12	35.78739676726267	37.0	35.0	39.0	32.0	39.0
13	35.24034382530006	37.0	35.0	39.0	30.0	39.0
14	36.12606897871049	38.0	35.0	40.0	31.0	41.0
15	36.524953526551435	38.0	35.0	41.0	31.0	41.0
16	36.74337761738597	38.0	35.0	41.0	32.0	41.0
17	36.70494734946022	38.0	35.0	40.0	32.0	41.0
18	36.67724251429961	38.0	35.0	40.0	32.0	41.0
19	36.63131079037715	37.0	35.0	40.0	32.0	41.0
20	36.48554976335304	37.0	35.0	40.0	32.0	41.0
21	36.336519168872826	37.0	35.0	40.0	31.0	41.0
22	36.21001126805134	37.0	35.0	40.0	31.0	41.0
23	36.17014846920327	36.0	35.0	40.0	31.0	41.0
24	36.07124975249173	36.0	35.0	40.0	31.0	41.0
25	35.95562863971244	36.0	35.0	40.0	31.0	41.0
26	35.798104768294266	36.0	34.0	40.0	31.0	41.0
27	35.674657254126515	36.0	34.0	40.0	30.0	41.0
28	35.672307098872345	36.0	34.0	40.0	30.0	41.0
29	35.80955562935486	36.0	35.0	40.0	30.0	41.0
30	35.834356963463534	36.0	35.0	40.0	30.0	41.0
31	35.690821387753715	36.0	34.0	40.0	30.0	41.0
32	35.54506304253477	36.0	34.0	40.0	30.0	41.0
33	35.36669349961717	36.0	34.0	40.0	30.0	41.0
34	35.26553491509181	36.0	34.0	40.0	29.0	41.0
35	35.124547948217916	36.0	34.0	40.0	29.0	41.0
36	34.946453290329096	35.0	34.0	40.0	27.0	41.0
37	34.80933706223442	35.0	34.0	40.0	27.0	41.0
38	34.795956642362064	35.0	34.0	40.0	27.0	41.0
39	34.76164518019272	35.0	34.0	40.0	27.0	41.0
40	34.587158444177554	35.0	34.0	40.0	26.0	41.0
41	34.57432243075244	35.0	34.0	40.0	26.0	41.0
42	34.54598692441198	35.0	34.0	40.0	27.0	41.0
43	34.40398355338289	35.0	33.0	40.0	27.0	41.0
44	34.29021735583844	35.0	33.0	40.0	26.0	41.0
45	34.25473975539255	35.0	33.0	39.0	26.0	41.0
46	34.28474200363921	35.0	33.0	39.0	27.0	41.0
47	34.27386236707744	35.0	33.0	39.0	27.0	41.0
48	34.24290941890199	35.0	34.0	39.0	27.0	41.0
49	34.258004853173404	35.0	34.0	39.0	27.0	41.0
50	34.109959822089046	35.0	34.0	39.0	26.0	41.0
51	33.84866618185589	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	1.0
13	7.0
14	26.0
15	56.0
16	143.0
17	338.0
18	855.0
19	1666.0
20	3076.0
21	4537.0
22	7293.0
23	10799.0
24	15504.0
25	22359.0
26	30250.0
27	36963.0
28	42462.0
29	49082.0
30	60034.0
31	75795.0
32	96842.0
33	131706.0
34	252283.0
35	392857.0
36	150560.0
37	184210.0
38	258841.0
39	408518.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.730046810909045	24.0258454502505	29.314633403939304	12.929474334901148
2	29.21464676826834	26.31481085004731	31.1582403603631	13.312302021321246
3	28.433883893033517	25.858769882791705	31.579864828080645	14.12748139609413
4	25.34645570395374	28.683783437081946	30.825115462886277	15.144645396078039
5	23.426461997256514	32.506115633180904	29.478536395895226	14.588885973667356
6	21.68704317125248	41.196549947056695	26.12914053955238	10.987266342138446
7	88.75528036261582	4.360659884977377	5.100927502314175	1.7831322500926339
8	90.69543230475676	2.7176072576799077	4.6684864204560945	1.918474017107235
9	87.39386197374603	3.5636720885317517	6.622047388391092	2.4204185493311354
10	59.42817656468805	17.20333312623838	11.556702970859059	11.811787338214517
11	51.245854934901416	18.562471980723185	16.80025781086927	13.391415273506132
12	45.07251824633185	19.49086822995049	21.617495024134012	13.819118499583649
13	21.42208082156207	42.1954776719607	21.98888034187652	14.393561164600706
14	15.298625708946368	43.69505372326184	26.546161241747303	14.460159326044487
15	13.717165206796231	20.73898035086057	51.02272874568844	14.52112569665476
16	15.388198001250617	15.541150288808067	48.52829237397415	20.542359335967163
17	16.45502009342515	16.303006437673282	27.4492591289765	39.79271433992506
18	22.356868706417874	21.82859778688499	33.16351547111048	22.65101803558666
19	31.245711905292943	22.73254491241448	23.15256029703674	22.86918288525584
20	32.87884185350281	21.579234603868326	22.203514148086597	23.33840939454226
21	23.149655008114696	27.767902278595752	25.707963039361303	23.37447967392825
22	23.901633174644964	24.03715372867015	23.300104277523925	28.761108819160963
23	21.191490274657077	31.134908655481453	22.349896013004965	25.323705056856504
24	21.48501384928881	23.41792491750097	36.9708295583201	18.126231674890125
25	18.266221904179996	23.54043871650593	34.952994660078964	23.24034471923511
26	17.90279260840862	34.127579728949954	25.44183857410208	22.52778908853935
27	19.189522723605563	32.06701473517845	27.12417964697611	21.619282894239884
28	16.277484591911946	27.54759198479953	38.04833417437723	18.126589248911298
29	16.48027375867061	23.63841399830778	37.356920107683415	22.524392135338193
30	19.9369865181185	28.033758563339095	30.772954352547426	21.25630056599498
31	29.982224101472355	24.578520796728558	23.311680736459454	22.127574365339637
32	32.07742907854516	24.96058863835366	24.14952136482428	18.812460918276905
33	28.73120669164023	25.32915806067942	24.31686600673401	21.622769240946337
34	20.610074916227113	25.03317616465211	28.395444685757248	25.961304233363535
35	21.058159861511584	24.507497656772742	31.001533545583314	23.432808936132364
36	31.877008839676773	22.25777600579985	25.208521525285622	20.656693629237754
37	21.858544611158365	29.69285732483678	27.53096479281491	20.91763327118995
38	22.08931394507395	31.74609205117421	22.285890263214707	23.878703740537137
39	21.586609568055053	31.375421881474047	24.320084172924584	22.71788437754632
40	25.5338691878019	24.207314265996633	23.624245127718734	26.634571418482732
41	18.440896813523807	23.245797723058025	25.232344894446385	33.08096056897178
42	22.980343709088505	26.349674317111837	22.762044769161385	27.907937204638273
43	23.225550094109014	26.978691717110674	24.050071090185085	25.745687098595226
44	21.49064564012231	30.052263912869936	26.505263713075454	21.9518267339323
45	18.297688418043364	34.76553647947816	21.678953059023403	25.257822043455075
46	22.37608831005601	30.9121847370423	25.69410704604078	21.017619906860908
47	23.33751545948932	25.408003132348426	26.57280050632481	24.68168090183744
48	24.993574841807018	20.70282067796928	30.065896422427222	24.23770805779648
49	20.702686587711344	21.43960194859963	31.953127409434323	25.90458405425471
50	19.159799383095418	31.819573512525594	25.53891992085099	23.481707183527995
51	18.932337608875702	33.44599894783844	22.991562594002858	24.630100849282996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	405.0
1	1176.0
2	1947.0
3	5277.0
4	8607.0
5	6641.0
6	4675.0
7	5163.5
8	5652.0
9	6061.0
10	6470.0
11	6578.5
12	6687.0
13	6467.5
14	6248.0
15	6119.5
16	5991.0
17	5622.5
18	5254.0
19	4957.5
20	4661.0
21	5031.5
22	5402.0
23	4968.0
24	4534.0
25	6058.5
26	9194.5
27	10806.0
28	12416.5
29	14027.0
30	15813.0
31	17599.0
32	23427.0
33	29255.0
34	33689.5
35	38124.0
36	42011.5
37	45899.0
38	59947.0
39	73995.0
40	112461.0
41	150927.0
42	189281.0
43	227635.0
44	232750.5
45	237866.0
46	221101.5
47	204337.0
48	193145.5
49	181954.0
50	173366.5
51	164779.0
52	154874.0
53	144969.0
54	130481.5
55	115994.0
56	108518.0
57	101042.0
58	97051.5
59	93061.0
60	90263.5
61	87466.0
62	78258.5
63	69051.0
64	62054.5
65	55058.0
66	46470.0
67	37882.0
68	31277.0
69	24672.0
70	19762.0
71	14852.0
72	12406.5
73	9961.0
74	8395.0
75	4903.0
76	2977.0
77	2123.5
78	1270.0
79	906.0
80	542.0
81	370.5
82	199.0
83	131.5
84	64.0
85	45.0
86	26.0
87	21.5
88	17.0
89	21.5
90	26.0
91	21.0
92	16.0
93	9.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2237299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.153799314879855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89021557407139	12.905305096060385
2	8.239645990790311	2.6620317552176216
3	2.564414426831887	1.2427510803367485
4	1.207715375319315	0.7803676720961207
5	0.7039916174112317	0.5686069653509358
6	0.4657125333413229	0.4513816081212014
7	0.35933983814248627	0.4063292541836593
8	0.2769912279261975	0.3579568566321551
9	0.20247839800399883	0.29437158662594703
>10	2.4719183786131813	9.906815566765456
>50	1.510390051694436	18.15289027404097
>100	2.09910667101607	50.97146963212086
>500	0.006965445550077284	0.7373556414936724
>1k	8.358534660092741E-4	0.2891723125555054
>5k	2.7861782200309135E-4	0.27319469839874205
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6070	0.2713092885662578	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4310	0.1926430039078371	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.469675264682995E-5	0.0	0.0	0.04255130851978211	0.0
2	4.469675264682995E-5	0.0	0.0	0.1597014972071234	0.0
3	4.469675264682995E-5	0.0	0.0	0.24364199867787006	0.0
4	4.469675264682995E-5	0.0	0.0	0.44660995244712487	0.0
5	4.469675264682995E-5	0.0	0.0	0.7454971373964767	0.0
6	4.469675264682995E-5	0.0	0.0	1.1280566433006942	0.0
7	4.469675264682995E-5	0.0	0.0	1.3282980951584924	0.0
8	4.469675264682995E-5	0.0	0.0	1.8947400414517683	0.0
9	4.469675264682995E-5	0.0	0.0	2.1415555095675636	0.0
10	4.469675264682995E-5	0.0	0.0	2.5085158487980372	0.0
11	4.469675264682995E-5	0.0	0.0	2.872436808848527	0.0
12	4.469675264682995E-5	0.0	0.0	3.1727095931299303	0.0
13	4.469675264682995E-5	0.0	0.0	3.2907090201175615	0.0
14	4.469675264682995E-5	0.0	0.0	3.3324557870897005	0.0
15	4.469675264682995E-5	0.0	0.0	3.398338800491128	0.0
16	4.469675264682995E-5	0.0	0.0	3.551916842585636	0.0
17	4.469675264682995E-5	0.0	0.0	3.721853896148883	0.0
18	4.469675264682995E-5	0.0	0.0	3.9732284330346546	0.0
19	4.469675264682995E-5	0.0	0.0	4.0853278886729045	0.0
20	4.469675264682995E-5	0.0	0.0	4.209316680515211	0.0
21	4.469675264682995E-5	0.0	0.0	4.378270405520228	0.0
22	4.469675264682995E-5	0.0	0.0	4.545659744182606	0.0
23	4.469675264682995E-5	0.0	0.0	4.7478678531568645	0.0
24	4.469675264682995E-5	0.0	0.0	4.8910315518846605	0.0
25	4.469675264682995E-5	0.0	0.0	5.00581281268172	0.0
26	4.469675264682995E-5	0.0	0.0	5.1186721131149655	0.0
27	4.469675264682995E-5	0.0	0.0	5.226391286993826	0.0
28	4.469675264682995E-5	0.0	0.0	5.344658894497338	0.0
29	4.469675264682995E-5	0.0	0.0	5.4672173902549455	0.0
30	4.469675264682995E-5	0.0	0.0	5.619588620027989	0.0
31	4.469675264682995E-5	0.0	0.0	5.775759073776013	0.0
32	4.469675264682995E-5	0.0	0.0	5.911905382338257	0.0
33	4.469675264682995E-5	0.0	0.0	6.048230477911089	0.0
34	4.469675264682995E-5	0.0	0.0	6.191885840918	0.0
35	4.469675264682995E-5	0.0	0.0	6.392306079786385	0.0
36	4.469675264682995E-5	0.0	0.0	6.546152302396774	0.0
37	4.469675264682995E-5	0.0	0.0	6.702501543155385	0.0
38	4.469675264682995E-5	0.0	0.0	6.85160990998521	0.0
39	4.469675264682995E-5	0.0	0.0	7.016004566220251	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	30	2.1664273E-6	45.000004	19
ATTACGC	55	1.8189894E-12	45.000004	22
CGTCGGA	30	2.1664273E-6	45.000004	23
TACGTCA	30	2.1664273E-6	45.000004	27
AATCCGT	20	7.03472E-4	45.0	35
TCGTTAG	85	0.0	45.0	1
GTACCGA	25	3.8919934E-5	45.0	3
CGTATAT	20	7.03472E-4	45.0	44
ACGCATA	20	7.03472E-4	45.0	33
CTATACG	20	7.03472E-4	45.0	1
ACTTACG	25	3.8919934E-5	45.0	41
CTAACGA	40	6.8193913E-9	45.0	38
CGTAGGT	20	7.03472E-4	45.0	40
CGTAAGT	25	3.8919934E-5	45.0	17
CCGGTAA	20	7.03472E-4	45.0	29
GCGTAAG	150	0.0	45.0	1
TACCGGT	25	3.8919934E-5	45.0	40
TATCCGA	20	7.03472E-4	45.0	23
ACGTTAG	140	0.0	43.392857	1
TACGTAG	145	0.0	41.89655	1
>>END_MODULE