FastQCFastQC Report
Thu 26 May 2016
SRR1545365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545365_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3035594
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC70000.23059737237588426No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58240.1918570138167357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC351.2128112E-745.00000433
GTCGACG453.8562575E-1045.01
TACGACG850.045.01
CCGCGTA207.035118E-445.014
GTCGGTA253.8923237E-545.026
TACCGGA253.8923237E-545.021
CGAACGT302.1666838E-644.99999638
ACGCTAA302.1666838E-644.99999638
CGTTTTT68600.041.4249271
TATAGCG1700.041.029411
TACGGGA7950.040.47174
TCTAGCG1300.039.807691
GCGTATG2450.039.4897961
GACGTGC403.4607183E-739.3759
TATTACG1600.039.3751
CTTAACG750.039.0000041
AATACGG2250.039.02
CGTTAGG3650.038.8356172
GGCACCG11250.038.68
GCGATCG356.2516774E-638.571439
Produced by FastQC (version 0.11.5)