##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545365_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3035594
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14991431660492	31.0	31.0	33.0	30.0	34.0
2	31.504052913531915	31.0	31.0	34.0	30.0	34.0
3	31.66216200190144	31.0	31.0	34.0	30.0	34.0
4	35.43697872640412	37.0	35.0	37.0	33.0	37.0
5	35.348603930565154	37.0	35.0	37.0	33.0	37.0
6	35.40354671935707	37.0	35.0	37.0	33.0	37.0
7	35.868191859649215	37.0	35.0	37.0	35.0	37.0
8	35.93543668883257	37.0	35.0	37.0	35.0	37.0
9	37.828093941416405	39.0	38.0	39.0	35.0	39.0
10	37.048227463883514	39.0	37.0	39.0	33.0	39.0
11	36.68360689868276	39.0	35.0	39.0	32.0	39.0
12	35.84117902459947	37.0	35.0	39.0	31.0	39.0
13	35.46856298964882	37.0	35.0	39.0	30.0	39.0
14	36.411650569871995	38.0	35.0	41.0	31.0	41.0
15	36.72109610178436	38.0	35.0	41.0	31.0	41.0
16	36.85445484475196	38.0	35.0	41.0	32.0	41.0
17	36.79587619424732	38.0	35.0	41.0	32.0	41.0
18	36.749201638954354	38.0	35.0	40.0	31.0	41.0
19	36.71565729804447	38.0	35.0	40.0	31.0	41.0
20	36.58827399184476	38.0	35.0	40.0	31.0	41.0
21	36.4605283183456	38.0	35.0	40.0	31.0	41.0
22	36.36284068291082	38.0	35.0	40.0	31.0	41.0
23	36.29652252573961	38.0	35.0	40.0	31.0	41.0
24	36.20683859567518	38.0	35.0	40.0	31.0	41.0
25	36.106397627614236	37.0	35.0	40.0	30.0	41.0
26	35.94102011006742	37.0	34.0	40.0	30.0	41.0
27	35.83253722335727	37.0	34.0	40.0	30.0	41.0
28	35.81989455770436	37.0	34.0	40.0	30.0	41.0
29	35.98056360633207	37.0	35.0	40.0	30.0	41.0
30	35.975890056443646	37.0	35.0	40.0	30.0	41.0
31	35.85280574411466	37.0	34.0	40.0	30.0	41.0
32	35.703224476000415	37.0	34.0	40.0	30.0	41.0
33	35.51938763879491	37.0	34.0	40.0	29.0	41.0
34	35.40974221190317	37.0	34.0	40.0	29.0	41.0
35	35.29395367101134	37.0	34.0	40.0	28.0	41.0
36	35.11372963578133	37.0	34.0	40.0	27.0	41.0
37	35.02565033400382	37.0	34.0	40.0	27.0	41.0
38	35.002090529892996	37.0	34.0	40.0	27.0	41.0
39	34.95634725855961	36.0	34.0	40.0	27.0	41.0
40	34.84326329542093	36.0	34.0	40.0	26.0	41.0
41	34.81878472549359	36.0	34.0	40.0	26.0	41.0
42	34.7634789105526	36.0	34.0	40.0	26.0	41.0
43	34.6340462525621	36.0	33.0	40.0	26.0	41.0
44	34.54303177565907	36.0	33.0	40.0	26.0	41.0
45	34.51203388858985	36.0	33.0	40.0	26.0	41.0
46	34.5349160658507	36.0	33.0	40.0	26.0	41.0
47	34.51886154736108	35.0	33.0	40.0	26.0	41.0
48	34.47464581890727	35.0	33.0	40.0	26.0	41.0
49	34.44050258367884	36.0	33.0	40.0	26.0	41.0
50	34.29968698053824	35.0	33.0	40.0	25.0	41.0
51	34.061422245530856	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	11.0
14	20.0
15	66.0
16	223.0
17	526.0
18	1226.0
19	2447.0
20	4411.0
21	7108.0
22	11309.0
23	16285.0
24	23583.0
25	33462.0
26	44276.0
27	52800.0
28	60108.0
29	70103.0
30	84925.0
31	104135.0
32	131105.0
33	169135.0
34	288755.0
35	432883.0
36	216556.0
37	265841.0
38	382047.0
39	631927.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.487943381097736	24.315471700102187	29.585807588234793	12.610777330565288
2	29.500980697682234	25.821404311643786	31.01735607594428	13.660258914729702
3	28.55866100670907	25.812773381420573	31.93071932544339	13.697846286426973
4	24.960024298374552	28.414076454229388	31.72963841673162	14.896260830664442
5	22.765000853210278	33.029087552551495	30.136704710840778	14.06920688339745
6	20.794974558521332	41.35954939955738	27.156266615364245	10.689209426557044
7	90.06741349469	3.6120113559323155	4.969571029590913	1.35100411978677
8	91.68274808818308	2.314637596463822	4.316585155985946	1.686029159367162
9	87.38918972695295	4.1863964680388746	6.14469523921842	2.27971856578976
10	49.404169332262484	23.968653252048856	14.486851667251946	12.140325748436714
11	43.503446113017745	22.804564773813627	19.39646738002513	14.295521733143495
12	37.58048671857963	22.50083509191282	24.527225972906784	15.39145221660077
13	21.106149241301704	39.6864995780068	24.736641329505858	14.470709851185633
14	17.375413181077576	40.5227115352053	27.056286183198413	15.045589100518713
15	16.095202454610202	24.547880908975312	44.37148050760411	14.985436128810376
16	17.417085420514073	20.045763695672083	42.73677573483147	19.800375148982376
17	18.05781669090135	21.12706771722437	27.326118051359966	33.48899754051431
18	22.960218000167348	24.02346954171078	32.313741560959734	20.702570897162136
19	29.005986966636517	25.769585787822745	24.189565534784954	21.034861710755788
20	30.890692233546385	24.02238243981244	24.296167405786147	20.79075792085503
21	22.971846696231445	28.504272969310122	27.28148098856435	21.242399345894082
22	24.17675090937721	25.491386529292125	24.50360621347914	25.828256347851525
23	20.924833821650722	31.04631251741834	23.949019532915138	24.0798341280158
24	21.02863558170164	25.987039109973203	34.90885144719616	18.07547386112899
25	18.687149862596907	27.035927729465797	33.10314225156592	21.17378015637137
26	19.15536135596526	33.8665183815754	26.009439997575434	20.968680264883908
27	19.45675212166054	32.01057190124898	27.78961876983549	20.74305720725499
28	16.814435659050584	28.3960569167023	35.80683055770963	18.98267686653749
29	17.51821883954178	24.605003172360995	35.311705056736834	22.565072931360387
30	20.00998816047205	29.284482707503045	30.006548965375472	20.698980166649427
31	27.645956606845317	27.035795959538728	24.19430925215954	21.123938181456413
32	28.969223156983443	27.171255444568676	24.927444183906015	18.932077214541863
33	26.5765118787295	27.55098343190822	24.451062955059207	21.421441734303073
34	20.347121518885594	27.448697026018632	28.155510914832483	24.048670540263288
35	21.29507437424109	26.816563743372797	29.371088492071074	22.517273390315044
36	29.421292834285477	25.211671916600174	25.49751383090097	19.86952141821337
37	21.781799542363043	30.689380727462236	28.05819882368986	19.47062090648486
38	21.872951389415054	31.83021181356927	24.49906015099516	21.797776646020512
39	21.483472427472186	31.221665347869315	26.243990467763478	21.050871756895027
40	24.183635888066718	26.78642137255509	24.780026577994292	24.2499161613839
41	19.30663323224384	24.63646324244942	26.68232313016826	29.374580395138477
42	22.991743955219306	26.54943315871622	23.85427695535042	26.604545930714053
43	22.574066228883048	27.67866849124092	25.79577506082829	23.951490219047738
44	21.13312913386968	30.592760428436737	26.646877019785915	21.627233417907664
45	19.17262321641168	34.32211290442661	22.950401140600487	23.554862738561216
46	22.426253313190102	32.074612085805946	24.98081100437015	20.518323596633806
47	22.448818913201173	26.909428599476744	26.99748385324256	23.644268634079523
48	23.873581249666458	23.58454391463417	29.102837863034388	23.439036972664987
49	21.602427729136373	23.656687949706054	30.563639274553843	24.17724504660373
50	20.267960735197132	31.38509299992028	26.235557192430868	22.11138907245172
51	19.79991395423762	31.68108119860561	24.179287480473345	24.339717366683423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	1092.5
2	1751.0
3	6333.5
4	10916.0
5	8549.5
6	6183.0
7	6613.5
8	7044.0
9	7667.5
10	8291.0
11	8475.0
12	8659.0
13	8662.5
14	8666.0
15	8513.5
16	8361.0
17	7977.5
18	7594.0
19	7894.0
20	8194.0
21	9072.0
22	9950.0
23	10000.0
24	10050.0
25	13178.0
26	17629.5
27	18953.0
28	24205.0
29	29457.0
30	34156.0
31	38855.0
32	48756.5
33	58658.0
34	67361.0
35	76064.0
36	84747.0
37	93430.0
38	110718.0
39	128006.0
40	172223.0
41	216440.0
42	250136.5
43	283833.0
44	290618.0
45	297403.0
46	282873.5
47	268344.0
48	264511.0
49	260678.0
50	257957.5
51	255237.0
52	241834.5
53	228432.0
54	202690.5
55	176949.0
56	155824.0
57	134699.0
58	119574.5
59	104450.0
60	92923.5
61	81397.0
62	72153.5
63	62910.0
64	50952.0
65	38994.0
66	33446.0
67	27898.0
68	21839.5
69	15781.0
70	13235.0
71	10689.0
72	8911.5
73	7134.0
74	5542.0
75	2934.0
76	1918.0
77	1527.0
78	1136.0
79	1021.5
80	907.0
81	625.5
82	344.0
83	225.0
84	106.0
85	93.5
86	81.0
87	53.5
88	26.0
89	28.0
90	30.0
91	18.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3035594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.12472186867161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38288969137946	14.990509646103995
2	8.809769728061942	3.3696879155245476
3	2.7734975427636677	1.5912710732639785
4	1.3069878945493238	0.9998311987590608
5	0.8304244781850594	0.7940818589113007
6	0.5568454071809678	0.638970812170992
7	0.43605288378368195	0.5837573085676572
8	0.3551869435428756	0.543428120531285
9	0.27995679297967463	0.4818686220883399
>10	3.442982803504735	15.649307607820267
>50	1.4189379239203668	19.838181026733483
>100	1.4010785042137581	39.414650355138875
>500	0.004520146912851997	0.5225694099460967
>1k	5.215554130213843E-4	0.15550404850276095
>5k	3.4770360868092287E-4	0.4263809959374399
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7000	0.23059737237588426	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5824	0.1918570138167357	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05112673170391034	0.0
2	0.0	0.0	0.0	0.17805411395595064	0.0
3	0.0	0.0	0.0	0.266998814729506	0.0
4	0.0	0.0	0.0	0.4261768866324021	0.0
5	0.0	0.0	0.0	0.7264805504293393	0.0
6	0.0	0.0	0.0	1.1172442691611593	0.0
7	0.0	0.0	0.0	1.3419449373005745	0.0
8	0.0	0.0	0.0	1.904701353343036	0.0
9	0.0	0.0	0.0	2.153417090691311	0.0
10	0.0	0.0	0.0	2.526358926786652	0.0
11	0.0	0.0	0.0	2.920680433549414	0.0
12	0.0	0.0	0.0	3.2337987227540967	0.0
13	0.0	0.0	0.0	3.375089027056978	0.0
14	0.0	0.0	0.0	3.4332325073774688	0.0
15	0.0	0.0	0.0	3.5196406370548896	0.0
16	0.0	0.0	0.0	3.7103776064915137	0.0
17	0.0	0.0	0.0	3.9471681654397788	0.0
18	0.0	0.0	0.0	4.240586850547207	0.0
19	0.0	0.0	0.0	4.403454480408118	0.0
20	0.0	0.0	0.0	4.563456114355214	0.0
21	0.0	0.0	0.0	4.797347734907896	0.0
22	0.0	0.0	0.0	5.033413559257266	0.0
23	0.0	0.0	0.0	5.314610583628773	0.0
24	0.0	0.0	0.0	5.526727223732818	0.0
25	0.0	0.0	0.0	5.709162687763911	0.0
26	0.0	0.0	0.0	5.888699213399421	0.0
27	0.0	0.0	0.0	6.060658968228294	0.0
28	0.0	0.0	0.0	6.252054787300278	0.0
29	0.0	0.0	0.0	6.457813528423102	0.0
30	0.0	0.0	0.0	6.716576722710613	0.0
31	0.0	0.0	0.0	6.938312567490909	0.0
32	0.0	0.0	0.0	7.150429207594955	0.0
33	0.0	0.0	0.0	7.357670360397339	0.0
34	0.0	0.0	0.0	7.584182865033993	0.0
35	0.0	0.0	0.0	7.859582012614335	0.0
36	3.294248176798347E-5	0.0	0.0	8.094330137692985	0.0
37	3.294248176798347E-5	0.0	0.0	8.334645542190424	0.0
38	3.294248176798347E-5	0.0	0.0	8.57716809296632	0.0
39	3.294248176798347E-5	0.0	0.0	8.821436595275916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	35	1.2128112E-7	45.000004	33
GTCGACG	45	3.8562575E-10	45.0	1
TACGACG	85	0.0	45.0	1
CCGCGTA	20	7.035118E-4	45.0	14
GTCGGTA	25	3.8923237E-5	45.0	26
TACCGGA	25	3.8923237E-5	45.0	21
CGAACGT	30	2.1666838E-6	44.999996	38
ACGCTAA	30	2.1666838E-6	44.999996	38
CGTTTTT	6860	0.0	41.424927	1
TATAGCG	170	0.0	41.02941	1
TACGGGA	795	0.0	40.4717	4
TCTAGCG	130	0.0	39.80769	1
GCGTATG	245	0.0	39.489796	1
GACGTGC	40	3.4607183E-7	39.375	9
TATTACG	160	0.0	39.375	1
CTTAACG	75	0.0	39.000004	1
AATACGG	225	0.0	39.0	2
CGTTAGG	365	0.0	38.835617	2
GGCACCG	1125	0.0	38.6	8
GCGATCG	35	6.2516774E-6	38.57143	9
>>END_MODULE