FastQCFastQC Report
Thu 26 May 2016
SRR1545364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545364_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4192072
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300600.7170678366211267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT242250.042.399381
CGATCGA750.039.010
GCGATAA5000.038.79
AGGGCGA50950.038.066736
GGGCGAT102450.038.0600287
AACACGT23650.038.05496641
CACGACC25550.037.77886627
GCGATAT5550.037.70279
GCGAGAC25750.037.66019421
CGTTAGG4250.037.5882382
AGACACG25950.037.45664624
GGCGATA21900.037.397268
TCAAGCG26100.037.24138317
CACGTGA24250.037.0206243
GTCAAGC26450.036.74858516
GACCGAT25550.036.7221159
ACACGAC26250.036.60000226
TAGGGAC32800.036.56255
ACGACCA26150.036.48183428
CGAGACA26400.036.47727222
Produced by FastQC (version 0.11.5)