##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545364_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4192072
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252383069756437	31.0	31.0	33.0	30.0	34.0
2	31.61970977597713	31.0	31.0	34.0	30.0	34.0
3	31.69676546586032	31.0	31.0	34.0	30.0	34.0
4	35.55192086395463	37.0	35.0	37.0	33.0	37.0
5	35.49615488474435	37.0	35.0	37.0	33.0	37.0
6	35.557285991271144	37.0	35.0	37.0	33.0	37.0
7	35.906304805833486	37.0	35.0	37.0	35.0	37.0
8	35.94931098511667	37.0	35.0	37.0	35.0	37.0
9	37.84085745664674	39.0	38.0	39.0	35.0	39.0
10	37.210206551795864	39.0	37.0	39.0	33.0	39.0
11	36.89266071765943	39.0	37.0	39.0	33.0	39.0
12	36.451317630040705	39.0	35.0	39.0	32.0	39.0
13	36.28269170949354	39.0	35.0	39.0	32.0	39.0
14	37.3009817102378	39.0	35.0	41.0	32.0	41.0
15	37.46057295771637	39.0	35.0	41.0	32.0	41.0
16	37.4930943457078	39.0	35.0	41.0	32.0	41.0
17	37.40329436135639	39.0	35.0	41.0	32.0	41.0
18	37.28936573608468	39.0	35.0	41.0	32.0	41.0
19	37.206081622643886	39.0	35.0	41.0	32.0	41.0
20	37.044276672728905	39.0	35.0	41.0	32.0	41.0
21	36.9460359936566	39.0	35.0	41.0	31.0	41.0
22	36.84517179094252	38.0	35.0	41.0	31.0	41.0
23	36.71859977595805	38.0	35.0	41.0	31.0	41.0
24	36.632796144722704	38.0	35.0	40.0	31.0	41.0
25	36.50073328893206	38.0	35.0	40.0	31.0	41.0
26	36.38664698507087	38.0	35.0	40.0	31.0	41.0
27	36.262493821671	38.0	35.0	40.0	30.0	41.0
28	36.16623474024301	38.0	35.0	40.0	30.0	41.0
29	36.233804906022606	38.0	35.0	40.0	30.0	41.0
30	36.17335914077812	38.0	35.0	40.0	30.0	41.0
31	36.0275956615249	38.0	35.0	40.0	30.0	41.0
32	35.83523231471215	38.0	35.0	40.0	29.0	41.0
33	35.54186545460097	38.0	34.0	40.0	27.0	41.0
34	35.26284496067816	38.0	34.0	40.0	26.0	41.0
35	35.038687074076975	38.0	34.0	40.0	25.0	41.0
36	34.87786206916294	38.0	34.0	40.0	24.0	41.0
37	34.75049211941016	38.0	34.0	40.0	24.0	41.0
38	34.63661287306134	37.0	33.0	40.0	24.0	41.0
39	34.55017304092105	37.0	33.0	40.0	23.0	41.0
40	34.42954128650462	37.0	33.0	40.0	23.0	41.0
41	34.32317670116353	37.0	33.0	40.0	23.0	41.0
42	34.235777200391595	37.0	33.0	40.0	23.0	41.0
43	34.1054731407285	37.0	33.0	40.0	23.0	41.0
44	33.96999693707551	36.0	33.0	40.0	22.0	41.0
45	33.928161300664684	36.0	33.0	40.0	22.0	41.0
46	33.92385793946287	36.0	33.0	40.0	23.0	41.0
47	33.8784195500459	36.0	33.0	40.0	23.0	41.0
48	33.79072878519262	36.0	33.0	40.0	22.0	41.0
49	33.73095905795511	36.0	33.0	40.0	22.0	41.0
50	33.620490774013426	35.0	33.0	40.0	22.0	41.0
51	33.38328253904036	35.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	6.0
11	2.0
12	5.0
13	21.0
14	61.0
15	121.0
16	342.0
17	900.0
18	1964.0
19	3999.0
20	7024.0
21	11104.0
22	17168.0
23	25414.0
24	38206.0
25	56860.0
26	78026.0
27	89052.0
28	93009.0
29	99397.0
30	113018.0
31	134115.0
32	163727.0
33	210031.0
34	327929.0
35	428288.0
36	340427.0
37	430370.0
38	602491.0
39	918639.0
40	352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.598921965080756	23.446472293414807	26.67246650343792	16.282139238066524
2	34.933536446893086	24.175515115198404	27.78318215908505	13.107766278823455
3	28.948262339005627	25.09348598974445	32.42637531034772	13.531876360902197
4	25.830138413653202	27.394734632420437	31.99014711579381	14.78497983813255
5	23.405537881982944	31.278351135190423	30.89259917291497	14.423511809911663
6	21.838603917108294	39.83500283392079	27.927502199389703	10.39889104958121
7	86.17468879351308	3.3254438377966795	8.787897726947438	1.7119696417427948
8	86.84951021833595	2.599287416819177	8.72000767162396	1.831194693220918
9	82.18961888059174	4.45667440826398	10.463918558650711	2.8897881524935642
10	41.71963172388261	28.11769454341433	17.328256766582253	12.83441696612081
11	33.29694718983834	25.502853958615212	25.355146571910026	15.845052279636418
12	28.822405722039125	23.35682688656111	30.12992143264715	17.690845958752615
13	23.59057764275041	28.097465883219563	31.36060163088802	16.951354843142006
14	20.74544521181888	29.60750674129643	32.168722292937716	17.478325753946976
15	20.102827432353262	23.691935634693298	38.20585619712639	17.999380735827057
16	23.083692264827512	21.799387033428815	36.099046008751756	19.017874692991914
17	22.661108874084224	22.084997585919325	30.65558034308571	24.59831319691074
18	24.886977132072158	22.917998545826503	31.95901692528182	20.23600739681952
19	27.34342349081791	24.43646006080048	28.114569597087076	20.10554685129454
20	29.153387632655164	24.18317242642779	27.410216236744024	19.25322370417302
21	26.550617451226984	24.927720707087094	28.94508968357414	19.576572158111787
22	25.669525714253	22.985697764733047	28.586698892576273	22.75807762843768
23	23.103205288458785	26.85974859210433	28.384889381670924	21.652156737765953
24	22.46347390979926	24.859496688033982	32.89454475018559	19.782484651981168
25	22.265361854471966	25.310204595722592	31.256452656347506	21.167980893457937
26	21.852129448158333	27.79983263646235	28.823240631363202	21.524797284016113
27	21.799506306189397	25.984167256669256	30.301531080573042	21.914795356568302
28	20.728055243325976	26.536805665551544	32.435392331047744	20.299746760074733
29	21.551275836865397	24.88366134932797	31.96388325391358	21.601179559893055
30	23.24315994572612	25.368886793929114	30.9605607918948	20.42739246844997
31	25.53980465984363	25.754829592621505	27.842031339156385	20.86333440837848
32	26.034643488947708	25.96367619640121	27.375531717966677	20.626148596684406
33	25.10999333980905	26.042253091072865	27.324626103750127	21.52312746536796
34	22.046066956865246	26.915854498682272	29.451092443068728	21.586986101383754
35	22.986174855775378	26.87709085149301	29.19234211626136	20.94439217647025
36	26.33950466499621	27.034435477253254	25.999791988305542	20.626267869444987
37	23.89147896314758	27.81564820451557	26.819625235444427	21.47324759689242
38	23.325839823361814	28.5201208376192	25.859551076412814	22.294488262606176
39	23.177989309343925	27.53430761685391	26.199216043999247	23.088487029802923
40	24.892272842642015	25.447201288527488	26.73162579268677	22.92890007614373
41	21.736577997706146	25.170249938455257	27.672163073534996	25.4210089903036
42	23.44888160317857	26.785942607855972	26.08194706579467	23.683228723170785
43	23.109502890217534	25.848244018709604	27.467443307271438	23.574809783801424
44	22.65552690888897	27.93313187368919	27.409906127566515	22.001435089855327
45	21.880587928833283	28.115476070067498	26.48205946844425	23.52187653265497
46	23.284905411929948	27.62860466137032	27.111342553276756	21.975147373422978
47	22.952349100874223	26.756577654200598	28.473652170096315	21.817421074828864
48	23.44420611096374	25.9255804766712	28.189043508794697	22.44116990357036
49	22.745267733951135	25.274542040308468	28.90374974475629	23.076440480984107
50	22.011716401817527	28.001451310950763	27.584211339881566	22.40262094735014
51	21.88189992919969	27.78461343221204	26.98949350106582	23.34399313752245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1279.0
1	2965.0
2	4651.0
3	29894.5
4	55138.0
5	39641.5
6	24145.0
7	23792.5
8	23440.0
9	24253.0
10	25066.0
11	25119.0
12	25172.0
13	24848.5
14	24525.0
15	23748.5
16	22972.0
17	22436.5
18	21901.0
19	21017.0
20	20133.0
21	20888.0
22	21643.0
23	22090.5
24	22538.0
25	24915.0
26	31203.0
27	35114.0
28	40602.5
29	46091.0
30	54488.0
31	62885.0
32	73873.5
33	84862.0
34	98157.5
35	111453.0
36	120006.5
37	128560.0
38	140193.0
39	151826.0
40	181810.5
41	211795.0
42	234302.0
43	256809.0
44	269820.0
45	282831.0
46	289654.5
47	296478.0
48	301498.0
49	306518.0
50	308379.5
51	310241.0
52	290045.0
53	269849.0
54	252340.0
55	234831.0
56	225195.0
57	215559.0
58	201966.5
59	188374.0
60	175871.5
61	163369.0
62	152508.5
63	141648.0
64	129243.5
65	116839.0
66	101270.5
67	85702.0
68	72759.5
69	59817.0
70	49921.0
71	40025.0
72	34533.5
73	29042.0
74	24491.5
75	15454.0
76	10967.0
77	8196.5
78	5426.0
79	4085.0
80	2744.0
81	2095.0
82	1446.0
83	1057.0
84	668.0
85	471.0
86	274.0
87	179.0
88	84.0
89	63.5
90	43.0
91	36.5
92	30.0
93	25.0
94	20.0
95	16.0
96	12.0
97	7.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4192072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.26267379820719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.90323734287512	16.776937972440738
2	6.828682440890475	2.9039209442439886
3	2.299075194009271	1.466534576633071
4	1.281832000081027	1.090207027273055
5	0.8507541666041533	0.9044654163484862
6	0.6138578946178298	0.7831356103027894
7	0.5148708694372803	0.7663271941530949
8	0.3980628819777872	0.6771104968534349
9	0.356895751852663	0.6829702156448165
>10	5.379565122972265	29.049348692242415
>50	2.165025803567878	32.26091408100765
>100	0.403944073047606	11.041092717060655
>500	0.0026086090684556912	0.37106756468326185
>1k	0.0014744312126000487	0.5010509385456644
>5k	0.0	0.0
>10k+	1.1341778558461913E-4	0.7249165525669091
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30060	0.7170678366211267	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.385455211647128E-5	0.0	0.0	0.032227499909352704	0.0
2	2.385455211647128E-5	0.0	0.0	0.10476919289554187	0.0
3	2.385455211647128E-5	0.0	0.0	0.19341270856034914	0.0
4	2.385455211647128E-5	0.0	0.0	0.29109709947729906	0.0
5	2.385455211647128E-5	0.0	0.0	0.5112984700644455	0.0
6	2.385455211647128E-5	0.0	0.0	0.9562574306929843	0.0
7	2.385455211647128E-5	0.0	0.0	1.2250743784934992	0.0
8	2.385455211647128E-5	0.0	0.0	1.7239684814573795	0.0
9	2.385455211647128E-5	0.0	0.0	2.0457902440606937	0.0
10	2.385455211647128E-5	0.0	0.0	2.407425254146398	0.0
11	2.385455211647128E-5	0.0	0.0	2.7076825016364223	0.0
12	2.385455211647128E-5	0.0	0.0	2.9419819125244033	0.0
13	2.385455211647128E-5	0.0	0.0	3.080052060174539	0.0
14	2.385455211647128E-5	0.0	0.0	3.1676459755462214	0.0
15	2.385455211647128E-5	0.0	0.0	3.2416427962115155	0.0
16	2.385455211647128E-5	0.0	0.0	3.3640882122253624	0.0
17	2.385455211647128E-5	0.0	0.0	3.494453339541878	0.0
18	2.385455211647128E-5	0.0	0.0	3.6601947676471207	0.0
19	2.385455211647128E-5	0.0	0.0	3.7625785053310152	2.385455211647128E-5
20	2.385455211647128E-5	0.0	0.0	3.8692560623958747	2.385455211647128E-5
21	2.385455211647128E-5	0.0	0.0	3.9955182067483572	2.385455211647128E-5
22	2.385455211647128E-5	0.0	0.0	4.138573955790836	2.385455211647128E-5
23	2.385455211647128E-5	0.0	0.0	4.299138946086804	2.385455211647128E-5
24	2.385455211647128E-5	0.0	0.0	4.430386691831629	2.385455211647128E-5
25	2.385455211647128E-5	0.0	0.0	4.547369415410804	2.385455211647128E-5
26	2.385455211647128E-5	0.0	0.0	4.662610756685477	2.385455211647128E-5
27	2.385455211647128E-5	0.0	0.0	4.785819518367051	2.385455211647128E-5
28	2.385455211647128E-5	0.0	0.0	4.910364134967147	2.385455211647128E-5
29	2.385455211647128E-5	0.0	0.0	5.04595340919717	2.385455211647128E-5
30	2.385455211647128E-5	0.0	0.0	5.222381676650592	2.385455211647128E-5
31	2.385455211647128E-5	0.0	0.0	5.370351463429063	2.385455211647128E-5
32	2.385455211647128E-5	0.0	0.0	5.5409830747181825	2.385455211647128E-5
33	2.385455211647128E-5	0.0	0.0	5.689119843361469	2.385455211647128E-5
34	2.385455211647128E-5	0.0	0.0	5.846536032778063	2.385455211647128E-5
35	2.385455211647128E-5	0.0	0.0	6.020316444946556	2.385455211647128E-5
36	2.385455211647128E-5	0.0	0.0	6.182789799411842	2.385455211647128E-5
37	2.385455211647128E-5	0.0	0.0	6.3425914440400835	2.385455211647128E-5
38	2.385455211647128E-5	0.0	0.0	6.5309946966559735	2.385455211647128E-5
39	2.385455211647128E-5	0.0	0.0	6.7934186244892745	2.385455211647128E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24225	0.0	42.39938	1
CGATCGA	75	0.0	39.0	10
GCGATAA	500	0.0	38.7	9
AGGGCGA	5095	0.0	38.06673	6
GGGCGAT	10245	0.0	38.060028	7
AACACGT	2365	0.0	38.054966	41
CACGACC	2555	0.0	37.778866	27
GCGATAT	555	0.0	37.7027	9
GCGAGAC	2575	0.0	37.660194	21
CGTTAGG	425	0.0	37.588238	2
AGACACG	2595	0.0	37.456646	24
GGCGATA	2190	0.0	37.39726	8
TCAAGCG	2610	0.0	37.241383	17
CACGTGA	2425	0.0	37.02062	43
GTCAAGC	2645	0.0	36.748585	16
GACCGAT	2555	0.0	36.722115	9
ACACGAC	2625	0.0	36.600002	26
TAGGGAC	3280	0.0	36.5625	5
ACGACCA	2615	0.0	36.481834	28
CGAGACA	2640	0.0	36.477272	22
>>END_MODULE